Accession	MF_01252
Dates	24-AUG-2004 (Created) 19-AUG-2008 (Last updated, Version 18)

Data class

Protein

Predictors

HAMAP; MF_01252; [distribution of match scores in UniProtKB];[seed alignment for MF_01252]

Identifier

HMP

Protein name

RecName: Full=Flavohemoprotein; AltName: Full=Hemoglobin-like protein; AltName: Full=Flavohemoglobin; AltName: Full=Nitric oxide dioxygenase; Short=NO oxygenase; Short=NOD; EC=1.14.12.17;

Gene name

hmp

FUNCTION: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a central role in the inducible response to nitrosative stress (By similarity).

CATALYTIC ACTIVITY: 2 NO + 2 O(2) + NAD(P)H = 2 NO(3)(-) + NAD(P)(+).

COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group per subunit (By similarity).

COFACTOR: Binds 1 FAD per subunit (By similarity).

DOMAIN: Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.

SIMILARITY: Belongs to the globin family. Two-domain flavohemoproteins subfamily.

SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.

Pfam	PF00970; FAD_binding_6; 1; PF00042; Globin; 1; PF00175; NAD_binding_1; 1;
PRINTS	PR00371; FPNCR; 1; PR00410; PHEHYDRXLASE; 1; PR00188; PLANTGLOBIN; 1; PR00406; CYTB5RDTASE; 1; PR00409; PHDIOXRDTASE; 1;
PROSITE	PS01033; GLOBIN; 1; trigger=PRU00238; PS51384; FAD_FR; 1; trigger=PRU00716;

Detoxification.

FAD, Flavoprotein, Heme, Iron, Metal-binding, NAD, NADP, Oxidoreductase, Oxygen transport, Transport.

GO:0005344; Molecular function: oxygen transporter activity.
GO:0008941; Molecular function: nitric oxide dioxygenase activity.
GO:0015671; Biological process: oxygen transport.

From: HMP_ECOLI (P24232)
Key		From		To		Description		Condition		FTGroup
DOMAIN		151		251		FAD-binding
NP_BIND		204		207		FAD (By similarity)		R-[QN]-Y-S
NP_BIND		268		273		NADP (By similarity)		G-[VI]-G-[QILAV]-T-P
NP_BIND		389		392		FAD (By similarity)		[CLFVT]-F-G-[PST]
REGION		147		Cter		Reductase
REGION		259		Cter		NAD or NADP-binding
ACT_SITE		95		95		Charge relay system (By similarity)		Y
ACT_SITE		135		135		Charge relay system (By similarity)		E
METAL		85		85		Iron (heme proximal ligand) (By similarity)		H
BINDING		188		188		FAD (By similarity)		Y
SITE		29		29		Involved in heme-bound ligand stabilization and O-O bond activation (By similarity)		Y
SITE		84		84		Influences the redox potential of the prosthetic heme and FAD groups (By similarity)		K
SITE		388		388		Influences the redox potential of the prosthetic heme and FAD groups (By similarity)		E

Size range:	392-411 amino acids
Related UniRules:	None
Template:	P24232 (HMP_ECOLI); P39662 (HMP_RALEH); P26353 (HMP_SALTY): [Recover all]
Scope:	Bacteria
Fusion:	Nter: None; Cter: None
Duplicate:	None
Plasmid encoded:	in RALEU, RHIME