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UniProtKB/Swiss-Prot entry Q8W4J9

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name RPP8_ARATH
Primary accession number Q8W4J9
Secondary accession numbers Q8GWG5 Q9M5A1 Q9ZSY3 Q9ZSY4
Integrated into Swiss-Prot on April 11, 2003
Sequence was last modified on April 11, 2003 (Sequence version 2)
Annotations were last modified on    November 25, 2008 (Entry version 57)
Name and origin of the protein
Protein name Disease resistance protein RPP8
Synonym Resistance to Peronospora parasitica protein 8
Gene name
Name: RPP8
Synonyms: HRT
OrderedLocusNames: At5g43470
ORFNames: MWF20.19
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, MUTANTS RPP8-1; RPP8-2 AND RPP8-3, AND VARIANTS.
STRAIN=cv. Columbia, and cv. Landsberg erecta;
DOI=10.1105/tpc.10.11.1861; PubMed=9811794 [NCBI, ExPASy, EBI, Israel, Japan]
McDowell J.M., Dhandaydham M., Long T.A., Aarts M.G.M., Goff S., Holub E.B., Dangl J.L.;
"Intragenic recombination and diversifying selection contribute to the evolution of downy mildew resistance at the RPP8 locus of Arabidopsis.";
Plant Cell 10:1861-1874(1998).
[2]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND VARIANTS.
STRAIN=cv. Di-17;
DOI=10.1105/tpc.12.5.663; PubMed=10810142 [NCBI, ExPASy, EBI, Israel, Japan]
Cooley M.B., Pathirana S., Wu H.-J., Kachroo P., Klessig D.F.;
"Members of the Arabidopsis HRT/RPP8 family of resistance genes confer resistance to both viral and oomycete pathogens.";
Plant Cell 12:663-676(2000).
[3]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.1.31; PubMed=10718197 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:31-63(2000).
[4]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
STRAIN=cv. Columbia;
DOI=10.1126/science.1071006; PubMed=11910074 [NCBI, ExPASy, EBI, Israel, Japan]
Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T., Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y., Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K., Shinagawa A., Shinozaki K.;
"Functional annotation of a full-length Arabidopsis cDNA collection.";
Science 296:141-145(2002).
[5]
 NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[6]
 INTERACTION WITH TIP.
DOI=10.1105/tpc.12.10.1917; PubMed=11041886 [NCBI, ExPASy, EBI, Israel, Japan]
Ren T., Qu F., Morris T.J.;
"HRT gene function requires interaction between a NAC protein and viral capsid protein to confer resistance to turnip crinkle virus.";
Plant Cell 12:1917-1926(2000).
Comments
  • FUNCTION: Disease resistance protein. Resistance proteins guard the plant against pathogens that contain an appropriate avirulence protein via an indirect interaction with this avirulence protein. That triggers a defense system including the hypersensitive response, which restricts the pathogen growth. The interaction with TIP (TCV-interacting protein) may be essential for the recognition of the avirulence proteins, and the triggering of the defense response.
  • SUBUNIT: Interacts with the NAC protein TIP.
  • ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.
    Name1
    Isoform IDQ8W4J9-1
    This is the isoform sequence displayed in this entry.
    Name2
    Isoform IDQ8W4J9-2
    Note: Has been shown to exist only in cv. Columbia so far.
    Features which should be applied to build the isoform sequence: VSP_007171, VSP_007172.
  • DOMAIN: The LRR repeats probably act as specificity determinant of pathogen recognition.
  • POLYMORPHISM: The strong polymorphisms present in cv. Di-17 and cv. Columbia are probably due to an unequal crossing-over between the highly related RPP8 and RPH8A genes present in cv. Landsberg erecta. Such variations probably modify the specificity of pathogen recognition.
  • MISCELLANEOUS: In cv. Columbia and cv. Landsberg erecta, RPP8 specifically recognizes the Emco5 avirulence protein from Peronospora parasitica, while it is not the case in cv. Di-17, where it confers resistance to Turnip Crinkle Virus uppon recognition of the viral capsid protein.
  • SIMILARITY: Belongs to the disease resistance NB-LRR family. RPP8/HRT subfamily.
  • SIMILARITY: Contains 2 LRR (leucine-rich) repeats.
  • SIMILARITY: Contains 1 NB-ARC domain.
  • WEB RESOURCE: Name=NIB-LRRS; Note=Functional and comparative genomics of disease resistance gene homologs; URL="http://niblrrs.ucdavis.edu";.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF089710; AAC83165.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF089711; AAC78631.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF234174; AAF36987.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB025638; BAA97426.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AK118862; BAC43449.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY062514; AAL32592.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T48898; T48898.
T48899; T48899.
RefSeq NP_199160.1; -.
NP_851124.1; -.
UniGene At.47555
3D structure databases
ModBase Q8W4J9.
Organism-specific databases
TAIR At5g43470; -.
Gene expression databases
ArrayExpress Q8W4J9; -.
Ontologies
GO
GO:0005524; Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0005515; Molecular function: protein binding (inferred from electronic annotation from InterPro).
GO:0006915; Biological process: apoptosis (inferred from electronic annotation from InterPro).
GO:0009626; Biological process: plant-type hypersensitive response (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
InterPro IPR000767; Disease_R.
IPR001611; LRR.
IPR002182; NB-ARC.
Graphical view of domain structure.
Pfam PF00560; LRR_1; 1.
PF00931; NB-ARC; 1.
Pfam graphical view of domain structure.
PRINTS PR00364; DISEASERSIST.
ProtoNet Q8W4J9.
Genome annotation databases
GeneID 834367; -.
GenomeReviews BA000015_GR; AT5G43470.
KEGG ath:AT5G43470; -.
NMPDR fig|3702.1.peg.26087; -.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; ATP-binding; Coiled coil; Complete proteome; Hypersensitive response; Leucine-rich repeat; Nucleotide-binding; Plant defense; Repeat.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   908  908     Disease resistance protein RPP8. PRO_0000212719
DOMAIN   146   459  314     NB-ARC. 
REPEAT   600   623  24     LRR 1. 
REPEAT   842   867  26     LRR 2. 
NP_BIND   192   199  8     ATP (Potential). 
COILED   15    57  43     Potential. 
VAR_SEQ   294   308        WKMLLTSRNEGVGIH -> ELLWYIHEALFLLNS (in isoform 2). VSP_007171
VAR_SEQ   309   908        Missing (in isoform 2). VSP_007172
VARIANT   27    31  5     IDGQL -> VDEQI (in strain: cv. Landsberg erecta). 
VARIANT   29    29  1     G -> E (in strain: cv. Di-17). 
VARIANT   85    87  3     SGK -> RGE (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   88    91  4     GKGV -> EKGI (in strain: cv. Landsberg erecta). 
VARIANT   100   100  1     C -> R (in strain: cv. Landsberg erecta). 
VARIANT   121   121  1     E -> D (in strain: cv. Landsberg erecta). 
VARIANT   129   129  1     F -> L (in strain: cv. Di-17). 
VARIANT   133   133  1     Q -> QQ (in strain: cv. Di-17). 
VARIANT   138   138  1     G -> V (in strain: cv. Landsberg erecta). 
VARIANT   173   173  1     K -> T (in strain: cv. Di-17). 
VARIANT   177   177  1     G -> C (in strain: cv. Di-17). 
VARIANT   253   253  1     Y -> S (in strain: cv. Di-17). 
VARIANT   257   257  1     R -> P (in strain: cv. Di-17). 
VARIANT   265   265  1     A -> T (in strain: cv. Di-17). 
VARIANT   267   267  1     R -> K (in strain: cv. Landsberg erecta). 
VARIANT   270   270  1     V -> L (in strain: cv. Di-17). 
VARIANT   283   283  1     V -> R (in strain: cv. Di-17). 
VARIANT   369   369  1     A -> V (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   387   387  1     F -> S (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   399   399  1     W -> C (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   426   426  1     C -> R (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   429   429  1     N -> F (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   436   446  11     DSEISTYSLFY -> YSKISAYDLFN (in strain: cv. Landsberg erecta). 
VARIANT   438   443  6     EISTYS -> KITTQE (in strain: cv. Di-17). 
VARIANT   450   450  1     A -> V (in strain: cv. Landsberg erecta). 
VARIANT   460   460  1     E -> Q (in strain: cv. Landsberg erecta). 
VARIANT   480   486  7     DNYLSWQ -> NKYLRVH (in strain: cv. Di-17). 
VARIANT   480   481  2     DN -> NR (in strain: cv. Landsberg erecta). 
VARIANT   485   486  2     WQ -> SH (in strain: cv. Landsberg erecta). 
VARIANT   489   489  1     Y -> N (in strain: cv. Landsberg erecta). 
VARIANT   514   514  1     I -> K (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   518   518  1     C -> S (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   519   519  1     T -> I (in strain: cv. Di-17). 
VARIANT   528   528  1     S -> R (in strain: cv. Di-17). 
VARIANT   537   537  1     S -> R (in strain: cv. Di-17). 
VARIANT   542   542  1     H -> Q (in strain: cv. Di-17). 
VARIANT   543   543  1     I -> L (in strain: cv. Landsberg erecta). 
VARIANT   547   550  4     KNKT -> RNNA (in strain: cv. Di-17). 
VARIANT   549   549  1     K -> N (in strain: cv. Landsberg erecta). 
VARIANT   558   565  8     PRFEEDYW -> WDEDFG (in strain: cv. Landsberg erecta). 
VARIANT   558   560  3     PRF -> SRFK (in strain: cv. Di-17). 
VARIANT   564   564  1     Y -> F (in strain: cv. Di-17). 
VARIANT   584   584  1     S -> Y (in strain: cv. Landsberg erecta). 
VARIANT   595   595  1     C -> S (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   609   612  4     YEAK -> FLAG (in strain: cv. Landsberg erecta). 
VARIANT   630   635  6     NLRVDT -> DLSVHE (in strain: cv. Di-17). 
VARIANT   632   636  5     RVDTE -> SVNNK (in strain: cv. Landsberg erecta). 
VARIANT   650   650  1     Q -> E (in strain: cv. Di-17). 
VARIANT   653   654  2     YL -> HI (in strain: cv. Di-17). 
VARIANT   659   659  1     Missing (in strain: cv. Di-17). 
VARIANT   660   660  1     M -> K (in strain: cv. Landsberg erecta). 
VARIANT   676   678  3     YLY -> FLF (in strain: cv. Landsberg erecta). 
VARIANT   678   679  2     YG -> FR (in strain: cv. Di-17). 
VARIANT   692   692  1     R -> H (in strain: cv. Landsberg erecta). 
VARIANT   697   697  1     R -> Q (in strain: cv. Di-17). 
VARIANT   700   700  1     A -> G (in strain: cv. Di-17). 
VARIANT   724   724  1     T -> S (in strain: cv. Di-17). 
VARIANT   726   727  2     NF -> YV (in strain: cv. Landsberg erecta). 
VARIANT   730   731  2     SL -> TP (in strain: cv. Di-17). 
VARIANT   731   734  4     LETY -> PEIF (in strain: cv. Landsberg erecta). 
VARIANT   752   752  1     Q -> E (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   768   768  1     F -> L (in strain: cv. Landsberg erecta). 
VARIANT   773   781  9     VHLFLIYCG -> AQIYICNCR (in strain: cv. Landsberg erecta). 
VARIANT   773   781  9     VHLFLIYCG -> THIHLLFCR (in strain: cv. Di-17). 
VARIANT   800   807  8     RLARKAFL -> QLTDEAFV (in strain: cv. Di-17). 
VARIANT   800   803  4     RLAR -> KLTF (in strain: cv. Landsberg erecta). 
VARIANT   807   809  3     LGS -> AGR (in strain: cv. Landsberg erecta). 
VARIANT   819   819  1     P -> T (in strain: cv. Landsberg erecta). 
VARIANT   823   824  2     VI -> AL (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   825   825  1     E -> D (in strain: cv. Di-17). 
VARIANT   828   829  2     KE -> EQ (in strain: cv. Landsberg erecta). 
VARIANT   851   851  1     D -> H (in strain: cv. Di-17 and cv. Landsberg erecta). 
VARIANT   854   854  1     K -> E (in strain: cv. Di-17 and cv. Landsberg erecta). 
MUTAGEN   418   418        D->N: In rpp8-1; loss of function. 
MUTAGEN   553   553        R->K: In rpp8-2; loss of function. 
MUTAGEN   830   830        S->L: In rpp8-1; loss of function. 
CONFLICT   83    83        K -> R (in Ref. 5; AAL32592). 
CONFLICT   686   686        S -> G (in Ref. 5; AAL32592). 
CONFLICT   835   835        W -> R (in Ref. 5; AAL32592). 
Sequence information
Length: 908 AA [This is the length of the unprocessed precursor] Molecular weight: 104682 Da [This is the MW of the unprocessed precursor] CRC64: 1BFA35BB6B0CB5CD [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAEAFVSFGL EKLWDLLSRE SERLQGIDGQ LDGLKRQLRS LQSLLKDADA KKHGSDRVRN 

        70         80         90        100        110        120 
FLEDVKDLVF DAEDIIESYV LNKLSGKGKG VKKHVRRLAC FLTDRHKVAS DIEGITKRIS 

       130        140        150        160        170        180 
EVIGEMQSFG IQQIIDGGRS LSLQERQRVQ REIRQTYPDS SESDLVGVEQ SVKELVGHLV 

       190        200        210        220        230        240 
ENDVHQVVSI AGMGGIGKTT LARQVFHHDL VRRHFDGFAW VCVSQQFTQK HVWQRILQEL 

       250        260        270        280        290        300 
QPHDGDILQM DEYALQRKLF QLLEAGRYLV VLDDVWKKED WDVIKAVFPR KRGWKMLLTS 

       310        320        330        340        350        360 
RNEGVGIHAD PTCLTFRASI LNPEESWKLC ERIVFPRRDE TEVRLDEEME AMGKEMVTHC 

       370        380        390        400        410        420 
GGLPLAVKAL GGLLANKHTV PEWKRVFDNI GSQIVGGSWL DDNSLNSVYR ILSLSYEDLP 

       430        440        450        460        470        480 
THLKHCFLNL AHFPEDSEIS TYSLFYYWAA EGIYDGSTIE DSGEYYLEEL VRRNLVIADD 

       490        500        510        520        530        540 
NYLSWQSKYC QMHDMMREVC LSKAKEENFL QIIIDPTCTS TINAQSPSRS RRLSIHSGKA 

       550        560        570        580        590        600 
FHILGHKNKT KVRSLIVPRF EEDYWIRSAS VFHNLTLLRV LDLSWVKFEG GKLPCSIGGL 

       610        620        630        640        650        660 
IHLRYLSLYE AKVSHLPSTM RNLKLLLYLN LRVDTEEPIH VPNVLKEMIQ LRYLSLPLKM 

       670        680        690        700        710        720 
DDKTKLELGD LVNLEYLYGF STQHSSVTDL LRMTKLRYLA VSLSERCNFE TLSSSLRELR 

       730        740        750        760        770        780 
NLETLNFLFS LETYMVDYMG EFVLDHFIHL KQLGLAVRMS KIPDQHQFPP HLVHLFLIYC 

       790        800        810        820        830        840 
GMEEDPMPIL EKLLHLKSVR LARKAFLGSR MVCSKGGFPQ LCVIEISKES ELEEWIVEEG 

       850        860        870        880        890        900 
SMPCLRTLTI DDCKKLKELP DGLKYITSLK ELKIEGMKRE WKEKLVPGGE DYYKVQHIPD 


VQFINCDQ
Q8W4J9 in FASTA format

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