NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 82-99; 353-359; 362-380; 518-538; 616-626 AND 1005-1009, AND SUBCELLULAR LOCATION.
STRAIN=MT-811;
DOI=10.1074/jbc.M005617200; PubMed=10995755 [NCBI, ExPASy, EBI, Israel, Japan]
Kitazume T., Takaya N., Nakayama N., Shoun H.;
"Fusarium oxysporum fatty-acid subterminal hydroxylase (CYP505) is a membrane-bound eukaryotic counterpart of Bacillus megaterium cytochrome P450BM3.";
J. Biol. Chem. 275:39734-39740(2000).

[2]

FUNCTION, AND SUBCELLULAR LOCATION.
STRAIN=MT-811;
PubMed=8830036 [NCBI, ExPASy, EBI, Israel, Japan]
Nakayama N., Takemae A., Shoun H.;
"Cytochrome P450foxy, a catalytically self-sufficient fatty acid hydroxylase of the fungus Fusarium oxysporum.";
J. Biochem. 119:435-440(1996).

Comments

FUNCTION: Functions as a fatty acid monooxygenase. Shows highest activity toward fatty acids with a chain length of 12-14 carbons. The reductase domain is required for electron transfer from NADP to cytochrome P450.
CATALYTIC ACTIVITY: RH + reduced flavoprotein + O₂ = ROH + oxidized flavoprotein + H₂O.
CATALYTIC ACTIVITY: NADPH + n oxidized hemoprotein = NADP⁺ + n reduced hemoprotein.
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Membrane; Peripheral membrane protein.
SIMILARITY: In the N-terminal section; belongs to the cytochrome P450 family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB030037; BAA82526.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P14779; 1JPZ. [HSSP ENTRY / PDB]

ModBase

Q9Y8G7.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR015702; NADPH_Cyt_Red.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19384:SF17; NADPH_Cyt_Red; 1.

Pfam

PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00369; FLAVODOXIN.
PR00371; FPNCR.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.
PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Electron transport; FAD; Flavoprotein; FMN; Heme; Iron; Membrane; Metal-binding; Monooxygenase; Multifunctional enzyme; NADP; Oxidoreductase; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1066`	`1066`	`Bifunctional P-450:NADPH-P450 reductase.`	`PRO_0000052210`
`DOMAIN`	`500 641`	`142`	`Flavodoxin-like.`
`DOMAIN`	`676 904`	`229`	`FAD-binding FR-type.`
`REGION`	`1 480`	`480`	`Cytochrome P450.`
`REGION`	`481 1066`	`586`	`NADPH-P-450 reductase.`
`METAL`	`407 407`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 1066 AA [This is the length of the unprocessed precursor]

Molecular weight: 117926 Da [This is the MW of the unprocessed precursor]

CRC64: 6B8123698C223DBA [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAESVPIPEP PGYPLIGNLG EFTSNPLSDL NRLADTYGPI FRLRLGAKAP IFVSSNSLIN 

        70         80         90        100        110        120 
EVCDEKRFKK TLKSVLSQVR EGVHDGLFTA FEDEPNWGKA HRILVPAFGP LSIRGMFPEM 

       130        140        150        160        170        180 
HDIATQLCMK FARHGPRTPI DTSDNFTRLA LDTLALCAMD FRFYSYYKEE LHPFIEAMGD 

       190        200        210        220        230        240 
FLTESGNRNR RPPFAPNFLY RAANEKFYGD IALMKSVADE VVAARKASPS DRKDLLAAML 

       250        260        270        280        290        300 
NGVDPQTGEK LSDENITNQL ITFLIAGHET TSGTLSFAMY QLLKNPEAYS KVQKEVDEVV 

       310        320        330        340        350        360 
GRGPVLVEHL TKLPYISAVL RETLRLNSPI TAFGLEAIDD TFLGGKYLVK KGEIVTALLS 

       370        380        390        400        410        420 
RGHVDPVVYG NDADKFIPER MLDDEFARLN KEYPNCWKPF GNGKRACIGR PFAWQESLLA 

       430        440        450        460        470        480 
MVVLFQNFNF TMTDPNYALE IKQTLTIKPD HFYINATLRH GMTPTELEHV LAGNGATSSS 

       490        500        510        520        530        540 
THNIKAAANL DAKAGSGKPM AIFYGSNSGT CEALANRLAS DAPSHGFSAT TVGPLDQAKQ 

       550        560        570        580        590        600 
NLPEDRPVVI VTASYEGQPP SNAAHFIKWM EDLDGNDMEK VSYAVFACGH HDWVETFHRI 

       610        620        630        640        650        660 
PKLVDSTLEK RGGTRLVPMG SADAATSDMF SDFEAWEDIV LWPGLKEKYK ISDEESGGQK 

       670        680        690        700        710        720 
GLLVEVSTPR KTSLRQDVEE ALVVAEKTLT KSGPAKKHIE IQLPSAMTYK AGDYLAILPL 

       730        740        750        760        770        780 
NPKSTVARVF RRFSLAWDSF LKIQSEGPTT LPTNVAISAF DVFSAYVELS QPATKRNILA 

       790        800        810        820        830        840 
LAEATEDKDT IQELERLAGD AYQAEISPKR VSVLDLLEKF PAVALPISSY LAMLPPMRVR 

       850        860        870        880        890        900 
QYSISSSPFA DPSKLTLTYS LLDAPSLSGQ GRHVGVATNF LSHLTAGDKL HVSVRASSEA 

       910        920        930        940        950        960 
FHLPSDAEKT PIICVAAGTG LAPLRGFIQE RAAMLAAGRT LAPALLFFGC RNPEIDDLYA 

       970        980        990       1000       1010       1020 
EEFERWEKMG AVDVRRAYSR ATDKSEGCKY VQDRVYHDRA DVFKVWDQGA KVFICGSREI 

      1030       1040       1050       1060 
GKAVEDVCVR LAIEKAQQNG RDVTEEMARA WFERSRNERF ATDVFD

Q9Y8G7 in FASTA format

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View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools