[1]	NUCLEOTIDE SEQUENCE [MRNA]. Tsoi S.C.-M., Li J.Y., Mannen H., Li S.S.-L.; "Molecular evolution of vertebrate lactate dehydrogenase isozymes by gene duplication."; Submitted (JUN-1998) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: (S)-lactate + NAD⁺ = pyruvate + NADH.
PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-lactate from pyruvate: step 1/1 .
SUBUNIT: Homotetramer (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF070998; AAD40735.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P00338; 1I10. [HSSP ENTRY / PDB]

SMR

Q9W7M6; 2-333.

ModBase

Q9W7M6.

Family and domain databases

InterPro

IPR001557; L-lactate/malate_DHase.
IPR011304; L-lactate_DHase.
IPR001236; Lactate/malate_DHase.
IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.90.110.10; lact_mal_DH; 1.
G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF02866; Ldh_1_C; 1.
PF00056; Ldh_1_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000102; Lac_mal_DH; 1.

PRINTS

PR00086; LLDHDRGNASE.

TIGRFAMs

TIGR01771; L-LDH-NAD; 1.

PROSITE

PS00064; L_LDH; 1.

BLOCKS

Q9W7M6.

Phylogenomic databases

HOVERGEN

Q9W7M6; -.

Other

ProtoNet

Q9W7M6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; Glycolysis; NAD; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 333`	`333`	`L-lactate dehydrogenase A chain.`	`PRO_0000168456`
`NP_BIND`	`30 58`	`29`	`NAD (By similarity).`
`ACT_SITE`	`194 194`		`Proton acceptor (By similarity).`
`BINDING`	`100 100`		`NAD (By similarity).`
`BINDING`	`107 107`		`Substrate (By similarity).`
`BINDING`	`139 139`		`NAD or substrate (By similarity).`
`BINDING`	`170 170`		`Substrate (By similarity).`
`BINDING`	`249 249`		`Substrate (By similarity).`

Sequence information

Length: 333 AA [This is the length of the unprocessed precursor]

Molecular weight: 36631 Da [This is the MW of the unprocessed precursor]

CRC64: EF97E41E545492C7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSCMKEQLII NILKDEHAPA QNKITVVGVG AVGMACAMSI LMKDLADELA LVDVIEDKLK 

        70         80         90        100        110        120 
GEMMDLQHGS LFLRTPKIVS GKDYSVTANS KLVIVTAGAR QQEGESRLNL VQRNVNIFKF 

       130        140        150        160        170        180 
IIPNVVKYSP DATLLVVSNP VDVLTYVAWK ISGFPKHRVI GSGCNLDSAR FRYLMGEKLG 

       190        200        210        220        230        240 
VHAQSCHGWV VGEHGDSSVP VWSGVNVAGV SLQTLNPELG TDADKENWKE VHKQVVESAY 

       250        260        270        280        290        300 
EVIKLKGYTS WAIGLSVADL AETIMKNLRR VHPVSTKVKG LYGVHEDVFL SVPCVLGNQG 

       310        320        330 
ITDVVKMTLK PEEEDRLRKS SDTLWGIQKE LHF

Q9W7M6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools