[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE. STRAIN=DAR 23471; DOI=10.1074/jbc.274.40.28219; PubMed=10497176 [NCBI, ExPASy, EBI, Israel, Japan] Hoernsten L., Su C., Osbourn A.E., Garosi P., Hellman U., Wernstedt C., Oliw E.H.; "Cloning of linoleate diol synthase reveals homology with prostaglandin H synthases."; J. Biol. Chem. 274:28219-28224(1999).
[2]	CHARACTERIZATION. DOI=10.1074/jbc.271.24.14112; PubMed=8662736 [NCBI, ExPASy, EBI, Israel, Japan] Su C., Oliw E.H.; "Purification and characterization of linoleate 8-dioxygenase from the fungus Gaeumannomyces graminis as a novel hemoprotein."; J. Biol. Chem. 271:14112-14118(1996).

Comments

FUNCTION: Catalyzes the dioxygenation of linoleic acid to (8R)-hydroperoxylinoleate and isomerization of the resulting hydroperoxide to (7S,8S)-dihydroxylinoleate.
CATALYTIC ACTIVITY: Linoleate + O₂ = (9Z,12Z)-(7S,8S)-dihydroxyoctadeca-9,12-dienoate.
COFACTOR: Heme group.
SUBUNIT: Homotetramer.
PTM: The N-terminus is blocked.
SIMILARITY: Belongs to the peroxidase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF124979; AAD49559.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q9UUS2.

Protein family/group databases

PeroxiBase

4150; GgrLDS.

Family and domain databases

InterPro

IPR002007; Haem_peroxidase_animal.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.640.10; Haem_peroxidase_animal; 1.

Pfam

PF03098; An_peroxidase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00457; ANPEROXIDASE.

PROSITE

PS50292; PEROXIDASE_3; 1.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Direct protein sequencing; Heme; Iron; Metal-binding; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 978`	`978`	`Linoleate diol synthase.`	`PRO_0000055599`
`ACT_SITE`	`376 376`		`Potential.`
`METAL`	`203 203`		`Iron (heme axial ligand) (Potential).`
`METAL`	`379 379`		`Iron (heme axial ligand) (Potential).`

Sequence information

Length: 978 AA [This is the length of the unprocessed precursor]

Molecular weight: 107965 Da [This is the MW of the unprocessed precursor]

CRC64: 104CC8541740ED3D [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTVSTHHDDS PGLSGRLRDL LHHVFGNQKS PTVYPNAPGN SAKPVPTGLA DDIDKLGFKD 

        70         80         90        100        110        120 
IDTLLIFLNS AVKGVNDDQQ FLLEKMIQLL AKLPPASREG KKLTDGLIND LWDSLDHPPV 

       130        140        150        160        170        180 
ASLGKGFSFR EPDGSNNNIH LPSLGAANTP YARSTKPLVF QNPNPPDPAT IFDTLMVRDP 

       190        200        210        220        230        240 
AKFRPHPNKI SSMLFYLATI ITHDIFQTSP RDFNINLTSS YLDLSPLYGR NHDEQMAVRT 

       250        260        270        280        290        300 
GKDGLLKPDT FSSKRVIGFP PGVGAFLIMF NRFHNYVVTQ LAKINEGGRF KRPTTPDDTA 

       310        320        330        340        350        360 
GWETYDNSLF QTGRLITCGL YINIVLGDYV RTILNLNRAN TTWNLDPRTK EGKSLLSKPT 

       370        380        390        400        410        420 
PEAVGNQVSV EFNLIYRWHC TISERDDKWT TNAMREALGG QDPATAKMED VMRALGMFEK 

       430        440        450        460        470        480 
NIPDEPEKRT LAGLTRQSDG AFDDTELVKI LQESIEDVAG AFGPNHVPAC MRAIEILGIK 

       490        500        510        520        530        540 
QSRTWNVATL NEFRQFIGLT PHDSFYHMNP DPKICKILAQ MYDSPDAVEL YPGIMAEAAK 

       550        560        570        580        590        600 
PPFSPGSGLC PPYTTSRAIL SDAVSLVRGD RFYTVDYTPR NITNWGFNEA STDKAVDWGH 

       610        620        630        640        650        660 
VIYKLFFRAF PNHFLPNSVY AHFPFVVPSE NKLIFEGLGA ANKYSWDPPK ARAPIQFIRS 

       670        680        690        700        710        720 
HKAVLEVLSN QKDYKVTWGP AIKMLSGDPA TSFALAGDEP ANAASRHHVI AALTAPKQWR 

       730        740        750        760        770        780 
DEVRRFYEVT TRDLLRRHGA PVHGVGAGPR THEVDVIRDV IGLAHARFMA SLFSLPLKEE 

       790        800        810        820        830        840 
GKEEGAYGEH ELYRSLVTIF AAIFWDSDVC NSLKLHQASK AAADKMSALI AEHVREMEAG 

       850        860        870        880        890        900 
TGFLGALGKL KDLITGNDVH ANGNGVYTNG NGVYTNGNGV HTNGNGVHTN GNGVPHAAPS 

       910        920        930        940        950        960 
LRSFGDQLLQ RMLSQDGRSI EETVSGTILP VVMAGTANQT QLLAQCLDYY LGVGEKHLPE 

       970 
MKRLAMLNTS EADEKLLK

Q9UUS2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools