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UniProtKB/Swiss-Prot entry Q9TUX8

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name NOS3_CANFA
Primary accession number Q9TUX8
Secondary accession numbers None
Integrated into Swiss-Prot on May 29, 2007
Sequence was last modified on May 1, 2000 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 43)
Name and origin of the protein
Protein name Nitric oxide synthase, endothelial
Synonyms EC 1.14.13.39
Endothelial NOS
eNOS
EC-NOS
NOS type III
NOSIII
Constitutive NOS
cNOS
Gene name
Name: NOS3
Synonyms: NOS
From
Canis familiaris (Dog) [TaxID: 9615] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Carnivora; Caniformia; Canidae; Canis.
Protein existence 2: Evidence at transcript level;
References
[1]
 NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Heart;
DOI=10.1006/niox.1999.0234; PubMed=10442857 [NCBI, ExPASy, EBI, Israel, Japan]
Schwemmer M., Bassenge E.;
"Assembly and characterization of canine heart endothelial nitric oxide synthase cDNA and 5'-flanking sequence by homology (RT-)PCR cloning.";
Nitric Oxide 3:254-264(1999).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF143503; AAD52161.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_001003158.1; -.
UniGene Cfa.108
3D structure databases
HSSP P29474; 1M9M. [HSSP ENTRY / PDB]
SMR Q9TUX8; 67-482.
ModBase Q9TUX8.
Family and domain databases
InterPro IPR003097; FAD-binding_1.
IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR001709; FPN_cyt_redctse.
IPR004030; NO_synthase_oxygenase_reg.
IPR012144; NOS.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.
Gene3D G3DSA:3.90.340.10; NO_synthase_oxygenase_reg; 1.
Pfam PF00667; FAD_binding_1; 1.
PF00258; Flavodoxin_1; 1.
PF00175; NAD_binding_1; 1.
PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF000333; NOS; 1.
PRINTS PR00369; FLAVODOXIN.
PR00371; FPNCR.
PROSITE PS51384; FAD_FR; 1.
PS50902; FLAVODOXIN_LIKE; 1.
PS60001; NOS; 1.
PROSITE graphical view of domain structure (profiles).
BLOCKS Q9TUX8.
Genome annotation databases
Ensembl ENSCAFG00000004687; Canis familiaris. [Contig view]
GeneID 403784; -.
KEGG cfa:403784; -.
Phylogenomic databases
HOVERGEN Q9TUX8; -.
Other
ProtoNet Q9TUX8.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Calcium; Calmodulin-binding; Cell membrane; Cytoplasm; Cytoskeleton; FAD; FMN; Golgi apparatus; Heme; Iron; Lipoprotein; Membrane; Metal-binding; Myristate; NADP; Oxidoreductase; Palmitate; Phosphoprotein; Zinc.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
INIT_MET   1      1        Removed (By similarity). 
CHAIN   2   1205  1204     Nitric oxide synthase, endothelial. PRO_0000289580
DOMAIN   522    705  184     Flavodoxin-like. 
DOMAIN   758   1004  247     FAD-binding FR-type. 
NP_BIND   651    682  32     FMN (By similarity). 
NP_BIND   795    806  12     FAD (By similarity). 
NP_BIND   937    947  11     FAD (By similarity). 
NP_BIND   1012   1030  19     NADP (By similarity). 
NP_BIND   1110   1125  16     NADP (By similarity). 
REGION   100    488  389     Interaction with NOSIP (By similarity). 
REGION   492    512  21     Calmodulin-binding (Potential). 
METAL   96     96        Zinc (By similarity). 
METAL   101    101        Zinc (By similarity). 
METAL   186    186        Iron (heme axial ligand) (By similarity). 
MOD_RES   83     83        Phosphotyrosine (By similarity). 
MOD_RES   497    497        Phosphothreonine (By similarity). 
MOD_RES   635    635        Phosphoserine (By similarity). 
MOD_RES   1177   1177        Phosphothreonine (By similarity). 
MOD_RES   1179   1179        Phosphoserine (By similarity). 
LIPID   2      2        N-myristoyl glycine (By similarity). 
LIPID   15     15        S-palmitoyl cysteine (By similarity). 
LIPID   26     26        S-palmitoyl cysteine (By similarity). 
Sequence information
Length: 1205 AA [This is the length of the unprocessed precursor] Molecular weight: 133046 Da [This is the MW of the unprocessed precursor] CRC64: C150CDEB01685BA5 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGNLKSVGQE PGPPCGLGLG LGLGLCGKQG PASPTSEPSR APALAPPPSP PPAPDHSSPP 

        70         80         90        100        110        120 
LTRPPDGPKF PRVKNWEVGS ITYDTLSAQS QQDGPCTPRR CLGSLVFPRK LQSRPSQNPA 

       130        140        150        160        170        180 
PPEQLLSQAR DFISQYYSSI KRSGSQAHEQ RLQEVEAEVA ATGTYQLRES ELVFGAKQAW 

       190        200        210        220        230        240 
RNAPRCVGRI QWGKLQVFDA RDCSSAQEMF TYICNHIKYA TNRGNLRSAI TVFPQRASGR 

       250        260        270        280        290        300 
GDFRIWNSQL VRYAGYRQQD GSVRGDPANV EITELCIQHG WTPGNGRFDV LPLLLQAPDE 

       310        320        330        340        350        360 
PPELFALPPE LVLEVPLEHP TLEWFAALGL RWYALPAVSN MLLEIGGLEF PAAPFSGWYM 

       370        380        390        400        410        420 
STEIGTRNLC DPHRYNILED VAVCMDLDTR TTSSLWKDKA AVEINLAVLH SYQLAKVTIV 

       430        440        450        460        470        480 
DHHAATASFM KHLENEQKAR GGCPADWAWI VPPISGSLTP VFHQEMVNYV LSPAFRYQTD 

       490        500        510        520        530        540 
PWKGSASKGA GVTRKKTFKE VANAVKISAS LMGTVMAKRV KATILYGSET GRAQSYAQQL 

       550        560        570        580        590        600 
GRLFRKAFDP RVLCMDEYDV VSLEHETLVL VVTSTFGNGD PPENGESFAA ALMEMSGSYN 

       610        620        630        640        650        660 
SSPRPEQHKS YKIRFNSVSC SDPLVSSWRR KRKESRNTDS AGALGTLRFC VFGLGSRAYP 

       670        680        690        700        710        720 
HFCAFARAVD TRLEELGGER LLQLGQGDEL CGQEEAFGGW AQAAFQASWE TFCVGEDAKA 

       730        740        750        760        770        780 
AARDIFSPKR TWKRQRYRLS AQAEGLQLLP GLIHVHRRKM VQATVLAVEN LQSSKSTRAT 

       790        800        810        820        830        840 
ILVRLDTGSQ EALQYQPGDH IGICPPNRPG LVEALLSRVE DPPPPGEPVA VEQLEKGSPG 

       850        860        870        880        890        900 
GPPPSWVRDP RLPPCTLRQA LTFFLDITSP PSPQLLRLLS TLAEESSEQQ ELESLSQDPR 

       910        920        930        940        950        960 
RYEEWKWFRC PTLLEVLEQF PSVALPAPLL LTQLPLLQPR YYSVSSAPSA HPGEIHLTVA 

       970        980        990       1000       1010       1020 
VLAYRTQDGL GPLHYGVCST WLSQLKAGDP VPCFIRGAPS FRLPPDPSLP CILVGPGTGI 

      1030       1040       1050       1060       1070       1080 
APFRGFWQGR LHDIYSKGLQ PAPMTLVFGC RCSQLDHLYR DEVQDAQQRG VFGRVLTAFS 

      1090       1100       1110       1120       1130       1140 
REPDSPKTYV QDILRTELAA EVHRVLCLER GHMFVCGDVT MATSVLQTVQ RILATEGDME 

      1150       1160       1170       1180       1190       1200 
LDEAGDVIGV LRDQQRYHED IFGLTLRTQE VTSRIRTQSF SLQERHLRGA VPWALDPPGP 


DTVGP
Q9TUX8 in FASTA format

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