NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBCELLULAR LOCATION.
DOI=10.1016/S0014-5793(00)01890-1; PubMed=11034343 [NCBI, ExPASy, EBI, Israel, Japan]
Kieselbach T., Bystedt M., Hynds P., Robinson C., Schroeder W.P.;
"A peroxidase homologue and novel plastocyanin located by proteomics to the Arabidopsis chloroplast thylakoid lumen.";
FEBS Lett. 480:271-276(2000).

Comments

FUNCTION: Plays a key role in hydrogen peroxide removal (By similarity).
CATALYTIC ACTIVITY: L-ascorbate + H₂O₂ = dehydroascorbate + 2 H₂O.
SUBCELLULAR LOCATION: Plastid, chloroplast thylakoid lumen.
SIMILARITY: Belongs to the peroxidase family. Ascorbate peroxidase subfamily.
CAUTION: Lacks the heme-binding site, the proton acceptor and the transition state stabilizer, which are conserved features of the ascorbate peroxidase.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ251882; CAB64343.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

Q43758; 1OAF. [HSSP ENTRY / PDB]

ModBase

Q9THX6.

Protein family/group databases

PeroxiBase

3921; LeAPx09.

Family and domain databases

InterPro

IPR002207; Asc_perxdse.
IPR002016; Haem_peroxidase_pln/fun/bac.
Graphical view of domain structure.

Pfam

PF00141; peroxidase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00459; ASPEROXIDASE.

PROSITE

PS00435; PEROXIDASE_1; FALSE_NEG.
PS00436; PEROXIDASE_2; FALSE_NEG.
PS50873; PEROXIDASE_4; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chloroplast; Hydrogen peroxide; Oxidoreductase; Peroxidase; Plastid; Thylakoid; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 ?`		`Chloroplast (Potential).`
`TRANSIT`	`? 78`		`Thylakoid (By similarity).`
`CHAIN`	`79 345`	`267`	`Putative L-ascorbate peroxidase, chloroplastic.`	`PRO_0000023636`

Sequence information

Length: 345 AA [This is the length of the unprocessed precursor]

Molecular weight: 37750 Da [This is the MW of the unprocessed precursor]

CRC64: 681CAEBFDDBEB41B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVSFASTLPS LVSFIPSPSS ITNASRNPPQ PGMICCKFRS ELNNEDRFHR RDILQSVGAA 

        70         80         90        100        110        120 
VGMDLIARSS AFIEVANAAD LIQRRQRSDF QSKIKLTLYD AIKANPDIIP SLLTLALNDA 

       130        140        150        160        170        180 
ITYDKATKTG GPNGSIRFSS EISRPENKGL DAALNLLEES KKVIDLDSKG GPISYADLIQ 

       190        200        210        220        230        240 
FAAQSAVKST FIASAISKCG GNVEKGTLLY SAYGSNGQWG QFDRIFGRSD AQEPDPEGRV 

       250        260        270        280        290        300 
PQWDKASVQE MKDKFKAVGL GPRQLAVMSS FLGPDQAATE ALLASDPEVL PWIQKYQRSR 

       310        320        330        340 
ETVSRTDYEV DLITTVTKLS SLGQVINYEA YTYPPRKIDV TKLKL

Q9THX6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools