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UniProtKB/Swiss-Prot entry Q9S818


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name FL3H_ARATH
Primary accession number Q9S818
Secondary accession numbers Q38877 Q9C526 Q9C528 Q9C530 Q9C563 Q9C5V7
Integrated into Swiss-Prot on December 13, 2002
Sequence was last modified on May 1, 2000 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 65)
Name and origin of the protein
Protein name Naringenin,2-oxoglutarate 3-dioxygenase
Synonyms Naringenin 3-dioxygenase
EC 1.14.11.9
Flavanone 3-hydroxylase
FH3
Protein TRANSPARENT TESTA 6
Gene name
Name: F3H
Synonyms: TT6
OrderedLocusNames: At3g51240
ORFNames: F24M12.280
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=cv. Landsberg erecta;
Pelletier M.K., Shirley B.W.;
"A genomic clone encoding flavanone 3-hydroxylase from Arabidopsis thaliana.";
(er) Plant Gene Register PGR95-080.
[2]
NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANT F3H-1(TT6).
STRAIN=cv. Columbia, and cv. Landsberg erecta;
DOI=10.1073/pnas.95.21.12432; PubMed=9770503 [NCBI, ExPASy, EBI, Israel, Japan]
Wisman E., Hartmann U., Sagasser M., Baumann E., Palme K., Hahlbrock K., Saedler H., Weisshaar B.;
"Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.";
Proc. Natl. Acad. Sci. U.S.A. 95:12432-12437(1998).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1038/35048706; PubMed=11130713 [NCBI, ExPASy, EBI, Israel, Japan]
Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B., Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M., Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V., Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P., Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H., Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H., Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A., Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H., Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J., Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B., Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D., de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E., Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G., Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X., Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M., Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B., Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J., Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C., Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y., Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K., Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.;
"Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
Nature 408:820-822(2000).
[4]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[5]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.;
"Full-length cDNA from Arabidopsis thaliana.";
Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
[6]
NUCLEOTIDE SEQUENCE OF 15-358.
STRAIN=cv. Can-0, cv. Cha-0, cv. Col-2, cv. Cvi-0, cv. Gr-5, cv. Ita-0, cv. Kas-1, cv. Kon, cv. La-0, cv. Me-0, cv. Mh-0, cv. Mr-0, cv. Nc-1, cv. Per-1, cv. Ri-0, cv. Rsch-0, cv. Rub-1, cv. Tul-0, cv. Wassilewskija, and cv. Yo-1;
TISSUE=Leaf;
PubMed=11141187 [NCBI, ExPASy, EBI, Israel, Japan]
Aguade M.;
"Nucleotide sequence variation at two genes of the phenylpropanoid pathway, the FAH1 and F3H genes, in Arabidopsis thaliana.";
Mol. Biol. Evol. 18:1-9(2001).
[7]
INTERACTION.
DOI=10.1073/pnas.96.22.12929; PubMed=10536025 [NCBI, ExPASy, EBI, Israel, Japan]
Burbulis I.E., Winkel-Shirley B.;
"Interactions among enzymes of the Arabidopsis flavonoid biosynthetic pathway.";
Proc. Natl. Acad. Sci. U.S.A. 96:12929-12934(1999).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U33932; AAC49176.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF064064; AAC68584.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF064065; AAC68585.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL132980; CAB62646.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AF428335; AAL16265.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY058886; AAL24272.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY116957; AAM51591.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY087559; AAM65101.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295606; CAC26961.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295604; CAC26959.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295605; CAC26960.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295603; CAC26958.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295587; CAC26942.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295588; CAC26943.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295589; CAC26944.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295590; CAC26945.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295591; CAC26946.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295592; CAC26947.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295593; CAC26948.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295601; CAC26956.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295602; CAC26957.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295596; CAC26951.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295594; CAC26949.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295595; CAC26950.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295599; CAC26954.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295597; CAC26952.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295598; CAC26953.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AJ295600; CAC26955.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
PIR T45755; T45755.
RefSeq NP_190692.1; -.
UniGene At.23201
3D structure databases
HSSP Q96323; 1GP4. [HSSP ENTRY / PDB]
ModBase Q9S818.
Protein-protein interaction databases
IntAct Q9S818; -.
Organism-specific databases
TAIR At3g51240; -.
Gene expression databases
ArrayExpress Q9S818; -.
GermOnline AT3G51240; Arabidopsis thaliana.
Family and domain databases
InterPro IPR005123; 2OG-FeII_Oase.
Graphical view of domain structure.
Pfam PF03171; 2OG-FeII_Oxy; 1.
Pfam graphical view of domain structure.
BLOCKS Q9S818.
Genome annotation databases
GeneID 824287; -.
GenomeReviews BA000014_GR; AT3G51240.
KEGG ath:AT3G51240; -.
NMPDR fig|3702.1.peg.16376; -.
Other
ProtoNet Q9S818.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
ATP-binding; Complete proteome; Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding; Nucleotide-binding; Oxidoreductase; Vitamin C.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
CHAIN   1   358  358     Naringenin,2-oxoglutarate 3-dioxygenase. PRO_0000067283
METAL   75    75        Iron (Potential). 
METAL   217   217        Iron (Potential). 
METAL   219   219        Iron (Potential). 
METAL   275   275        Iron (Potential). 
VARIANT   77    77  1     V -> L (in strain: cv. Ri-0, cv. Tul-0 and cv. Yo-1). 
VARIANT   148   148  1     D -> N (in strain: cv. Cha-0, cv. Con, cv. Cvi-0, cv. Gr-5, cv. Ita-0, cv. Kas-1, cv. La-0, cv. Ler, cv. Me-0, cv. Mh-0, cv. Nc-1, cv. Per-1, cv. Ri-0, cv. Rsch-0, cv. Rub-1, cv. Tul-0, cv. Ws-0 and cv. Yo-1). 
VARIANT   158   158  1     E -> K (in strain: cv. Me-0, cv. Mh-0, cv. Per-1 and cv. Ws-0). 
VARIANT   350   350  1     D -> A (in strain: cv. Cha-0, cv. Con, cv. Cvi-0, cv. Gr-5, cv. Ita-0, cv. Kas-1, cv. La-0, cv. Ler, cv. Me-0, cv. Mh-0, cv. Nc-1, cv. Per-1, cv. Ri-0, cv. Rsch-0, cv. Rub-1, cv. Tul-0, cv. Ws-0 and cv. Yo-1). 
VARIANT   354   354  1     D -> N (in strain: cv. Me-0). 
MUTAGEN   248   358        Missing: In f3h-1(tt6); reduced seed pigmentation. 
Sequence information
Length: 358 AA [This is the length of the unprocessed precursor] Molecular weight: 40276 Da [This is the MW of the unprocessed precursor] CRC64: 16BB0A3B318E5524 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAPGTLTELA GESKLNSKFV RDEDERPKVA YNVFSDEIPV ISLAGIDDVD GKRGEICRQI 

        70         80         90        100        110        120 
VEACENWGIF QVVDHGVDTN LVADMTRLAR DFFALPPEDK LRFDMSGGKK GGFIVSSHLQ 

       130        140        150        160        170        180 
GEAVQDWREI VTYFSYPVRN RDYSRWPDKP EGWVKVTEEY SERLMSLACK LLEVLSEAMG 

       190        200        210        220        230        240 
LEKESLTNAC VDMDQKIVVN YYPKCPQPDL TLGLKRHTDP GTITLLLQDQ VGGLQATRDN 

       250        260        270        280        290        300 
GKTWITVQPV EGAFVVNLGD HGHFLSNGRF KNADHQAVVN SNSSRLSIAT FQNPAPDATV 

       310        320        330        340        350 
YPLKVREGEK AILEEPITFA EMYKRKMGRD LELARLKKLA KEERDHKEVD KPVDQIFA 

Q9S818 in FASTA format

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