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[1]
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NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION IN CATABOLISM OF 3-HYDROXY DERIVATIVES OF PHENYLPROPIONIC ACID, AND BIOPHYSICOCHEMICAL PROPERTIES.
STRAIN=TA441;
PubMed=10537203 [NCBI, ExPASy, EBI, Israel, Japan]
Arai H.,
Yamamoto T.,
Ohishi T.,
Shimizu T.,
Nakata T.,
Kudo T.;
"Genetic organization and characteristics of the 3-(3-hydroxyphenyl)propionic acid degradation pathway of Comamonas testosteroni TA441.";
Microbiology 145:2813-2820(1999).
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- FUNCTION: Catalyzes the non-heme iron(II)-dependent oxidative cleavage of 2,3-dihydroxyphenylpropionic acid and 2,3-dihydroxicinnamic acid into 2-hydroxy-6-ketononadienedioate and 2-hydroxy-6-ketononatrienedioate, respectively.
- CATALYTIC ACTIVITY: 3-(2,3-dihydroxyphenyl)propanoate + O2 = 2-hydroxy-6-oxonona-2,4-diene-1,9-dioate.
- CATALYTIC ACTIVITY: 2,3-dihydroxicinnamic acid + O2 = 2-hydroxy-6-oxonona-2,4,7-triene-1,9-dioate.
- COFACTOR: Fe(2+) ion (By similarity).
- BIOPHYSICOCHEMICAL PROPERTIES:
| Kinetic parameters: |
Vmax=0.73 µmol/min/mg enzyme with 3-(2,3- dihydroxyphenyl)propanoate (at pH 7.5); | | Vmax=0.45 µmol/min/mg enzyme withd 2,3-dihydroxybiphenyl (at pH 7.5); | | Vmax=0.22 µmol/min/mg enzyme withd 3-methylcatechol (at pH 7.5); | | Vmax=0.10 µmol/min/mg enzyme withd catechol (at pH 7.5); | |
- PATHWAY: Aromatic compound metabolism; 3-phenylpropionic acid degradation.
- SUBUNIT: Homotetramer (By similarity).
- SIMILARITY: Belongs to the ligB/mhpB extradiol dioxygenase family.
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Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms.
Distributed under the Creative Commons Attribution-NoDerivs License.
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| Length: 321 AA [This is the length of the unprocessed precursor] |
Molecular weight: 34366 Da [This is the MW of the unprocessed precursor] |
CRC64: 6FC238E939E5CF64 [This is a checksum on the sequence] |
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10 20 30 40 50 60
MSGSAIATAR RAFLGMSHSP LLGLNPVAAD DQIAIDKAIA AARAAVHEFA PELIVLLGPD
70 80 90 100 110 120
HYNGFFNELM PPFCIGSQAT AVGDYLSPAG PLNVAGELAI ALADHLMDRH FDIAVSRRML
130 140 150 160 170 180
VDHGFSQALQ FLWGDEMDTP PVIPIFMNAV AQPGIARMAR CKALGEGVGS FLDQLPLRTL
190 200 210 220 230 240
LIGSGGLSHE PPVPTLAHPD PAVRERITVR STPTEQEREL KTERVKAAGL ALAHGDSWMK
250 260 270 280 290 300
PLNPEWDLQW MDAMASGQLD GLCAMNEASI GAMAGNSAHE SKTWLVARSA LPANTRLSCP
310 320
VRAYRAIPSL IAGYGVMFMH H
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Q9S157 in FASTA format |
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