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UniProtKB/Swiss-Prot entry Q9P2M7


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name CING_HUMAN
Primary accession number Q9P2M7
Secondary accession numbers Q5T386 Q9NR25
Integrated into Swiss-Prot on January 27, 2003
Sequence was last modified on January 27, 2003 (Sequence version 2)
Annotations were last modified on    September 23, 2008 (Entry version 57)
Name and origin of the protein
Protein name Cingulin
Synonyms None
Gene name
Name: CGN
Synonyms: KIAA1319
From
Homo sapiens (Human) [TaxID: 9606] 
Taxonomy Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Neuroepithelium;
DOI=10.1006/jsbi.2000.4284; PubMed=11042084 [NCBI, ExPASy, EBI, Israel, Japan]
Citi S., D'Atri F., Parry D.A.D.;
"Human and Xenopus cingulin share a modular organization of the coiled-coil rod domain: predictions for intra- and intermolecular assembly.";
J. Struct. Biol. 131:135-145(2000).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
TISSUE=Brain;
DOI=10.1093/dnares/7.1.65; PubMed=10718198 [NCBI, ExPASy, EBI, Israel, Japan]
Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
"Prediction of the coding sequences of unidentified human genes. XVI. The complete sequences of 150 new cDNA clones from brain which code for large proteins in vitro.";
DNA Res. 7:65-73(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1038/nature04727; PubMed=16710414 [NCBI, ExPASy, EBI, Israel, Japan]
Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A., Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C., Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K., Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C., Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W., Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J., Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J., Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y., Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J., Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H., Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L., Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J., Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S., Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K., Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R., Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M., Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S., Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J., Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W., McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N., Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V., Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J., Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E., Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S., Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M., White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H., Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E., Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G., Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
"The DNA sequence and biological annotation of human chromosome 1.";
Nature 441:315-321(2006).
[4]
PROTEIN SEQUENCE OF 368-376.
TISSUE=Platelet;
DOI=10.1038/nbt810; PubMed=12665801 [NCBI, ExPASy, EBI, Israel, Japan]
Gevaert K., Goethals M., Martens L., Van Damme J., Staes A., Thomas G.R., Vandekerckhove J.;
"Exploring proteomes and analyzing protein processing by mass spectrometric identification of sorted N-terminal peptides.";
Nat. Biotechnol. 21:566-569(2003).
[5]
INTERACTION WITH TJP1.
DOI=10.1074/jbc.M203717200; PubMed=12023291 [NCBI, ExPASy, EBI, Israel, Japan]
D'Atri F., Nadalutti F., Citi S.;
"Evidence for a functional interaction between cingulin and ZO-1 in cultured cells.";
J. Biol. Chem. 277:27757-27764(2002).
[6]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-134, AND MASS SPECTROMETRY.
DOI=10.1021/pr050048h; PubMed=16083285 [NCBI, ExPASy, EBI, Israel, Japan]
Kim J.-E., Tannenbaum S.R., White F.M.;
"Global phosphoproteome of HT-29 human colon adenocarcinoma cells.";
J. Proteome Res. 4:1339-1346(2005).
[7]
PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-131, AND MASS SPECTROMETRY.
DOI=10.1021/pr0705441; PubMed=18220336 [NCBI, ExPASy, EBI, Israel, Japan]
Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III;
"Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis.";
J. Proteome Res. 7:1346-1351(2008).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AF263462; AAF74498.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB037740; BAA92557.1; ALT_INIT; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AL365436; CAI16590.1; ALT_INIT; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_065821.1; -.
UniGene Hs.591464
3D structure databases
HSSP O46345; 1L4A. [HSSP ENTRY / PDB]
ModBase Q9P2M7.
Protein-protein interaction databases
IntAct Q9P2M7; -.
PTM databases
PhosphoSite Q9P2M7; -.
Organism-specific databases
H-InvDB HIX0001061; -.
HGNC HGNC:17429; CGN.
GenAtlas CGN.
HPA CAB017193; -.
MIM 609473; gene. [NCBI / EBI]
PharmGKB PA134938123; -.
GeneCards Q9P2M7.
HUGE KIAA1319.
Gene expression databases
ArrayExpress Q9P2M7; -.
CleanEx HS_CGN; -.
GermOnline ENSG00000143375; Homo sapiens.
Ontologies
GO
GO:0005923; Cellular component: tight junction (non-traceable author statement from UniProtKB).
GO:0003779; Molecular function: actin binding (inferred from sequence or structural similarity from UniProtKB).
QuickGo view.
Family and domain databases
InterPro IPR002928; Myosin_tail.
Graphical view of domain structure.
Pfam PF01576; Myosin_tail_1; 1.
Pfam graphical view of domain structure.
BLOCKS Q9P2M7.
Genome annotation databases
Ensembl ENSG00000143375; Homo sapiens. [Contig view]
GeneID 57530; -.
Phylogenomic databases
HOVERGEN Q9P2M7; -.
Other
SOURCE CGN; Homo sapiens.
ProtoNet Q9P2M7.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Cell junction; Coiled coil; Direct protein sequencing; Phosphoprotein; Tight junction.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom    To Length Description FTId
CHAIN   1   1197  1197     Cingulin. PRO_0000089763
REGION   1    351  351     Head. 
REGION   106    400  295     Interacts with ZO-2. 
REGION   1155   1197  43     Tail. 
COILED   352   1154  803     Potential. 
MOTIF   42     56  15     ZIM. 
COMPBIAS   363    836  474     Glu-rich. 
MOD_RES   111    111        Phosphoserine (By similarity). 
MOD_RES   112    112        Phosphoserine (By similarity). 
MOD_RES   129    129        Phosphoserine (By similarity). 
MOD_RES   131    131        Phosphoserine. 
MOD_RES   134    134        Phosphoserine. 
Sequence information
Length: 1197 AA [This is the length of the unprocessed precursor] Molecular weight: 136386 Da [This is the MW of the unprocessed precursor] CRC64: 0C9375283ABAAF3D [This is a checksum on the sequence]
        10         20         30         40         50         60 
MAEPRGPVDH GVQIRFITEP VSGAEMGTLR RGGRRPAKDA RASTYGVAVR VQGIAGQPFV 

        70         80         90        100        110        120 
VLNSGEKGGD SFGVQIKGAN DQGASGALSS DLELPENPYS QVKGFPAPSQ SSTSDEEPGA 

       130        140        150        160        170        180 
YWNGKLLRSH SQASLAGPGP VDPSNRSNSM LELAPKVASP GSTIDTAPLS SVDSLINKFD 

       190        200        210        220        230        240 
SQLGGQARGR TGRRTRMLPP EQRKRSKSLD SRLPRDTFEE RERQSTNHWT SSTKYDNHVG 

       250        260        270        280        290        300 
TSKQPAQSQN LSPLSGFSRS RQTQDWVLQS FEEPRRSAQD PTMLQFKSTP DLLRDQQEAA 

       310        320        330        340        350        360 
PPGSVDHMKA TIYGILREGS SESETSVRRK VSLVLEKMQP LVMVSSGSTK AVAGQGELTR 

       370        380        390        400        410        420 
KVEELQRKLD EEVKKRQKLE PSQVGLERQL EEKTEECSRL QELLERRKGE AQQSNKELQN 

       430        440        450        460        470        480 
MKRLLDQGED LRHGLETQVM ELQNKLKHVQ GPEPAKEVLL KDLLETRELL EEVLEGKQRV 

       490        500        510        520        530        540 
EEQLRLRERE LTALKGALKE EVASRDQEVE HVRQQYQRDT EQLRRSMQDA TQDHAVLEAE 

       550        560        570        580        590        600 
RQKMSALVRG LQRELEETSE ETGHWQSMFQ KNKEDLRATK QELLQLRMEK EEMEEELGEK 

       610        620        630        640        650        660 
IEVLQRELEQ ARASAGDTRQ VEVLKKELLR TQEELKELQA ERQSQEVAGR HRDRELEKQL 

       670        680        690        700        710        720 
AVLRVEADRG RELEEQNLQL QKTLQQLRQD CEEASKAKMV AEAEATVLGQ RRAAVETTLR 

       730        740        750        760        770        780 
ETQEENDEFR RRILGLEQQL KETRGLVDGG EAVEARLRDK LQRLEAEKQQ LEEALNASQE 

       790        800        810        820        830        840 
EEGSLAAAKR ALEARLEEAQ RGLARLGQEQ QTLNRALEEE GKQREVLRRG KAELEEQKRL 

       850        860        870        880        890        900 
LDRTVDRLNK ELEKIGEDSK QALQQLQAQL EDYKEKARRE VADAQRQAKD WASEAEKTSG 

       910        920        930        940        950        960 
GLSRLQDEIQ RLRQALQASQ AERDTARLDK ELLAQRLQGL EQEAENKKRS QDDRARQLKG 

       970        980        990       1000       1010       1020 
LEEKVSRLET ELDEEKNTVE LLTDRVNRGR DQVDQLRTEL MQERSARQDL ECDKISLERQ 

      1030       1040       1050       1060       1070       1080 
NKDLKTRLAS SEGFQKPSAS LSQLESQNQL LQERLQAEER EKTVLQSTNR KLERKVKELS 

      1090       1100       1110       1120       1130       1140 
IQIEDERQHV NDQKDQLSLR VKALKRQVDE AEEEIERLDG LRKKAQREVE EQHEVNEQLQ 

      1150       1160       1170       1180       1190 
ARIKSLEKDS WRKASRSAAE SALKNEGLSS DEEFDSVYDP SSIASLLTES NLQTSSC 

Q9P2M7 in FASTA format

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