ID   FLS_EUSGR               Reviewed;         334 AA.
AC   Q9M547;
DT   11-JUL-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   04-NOV-2008, entry version 39.
DE   RecName: Full=Flavonol synthase/flavanone 3-hydroxylase;
DE            Short=FLS;
DE            EC=1.14.11.23;
DE            EC=1.14.11.9;
GN   Name=FLS;
OS   Eustoma grandiflorum (Bluebells) (Lisianthus russellianus).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   asterids; lamiids; Gentianales; Gentianaceae; Chironieae; Eustoma.
OX   NCBI_TaxID=52518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RA   Nielsen K.M.;
RT   "cDNA cloning of flavonol synthase and antisense suppression of
RT   expression in Lisianthus grandiflorum grise.";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the formation of flavonols from
CC       dihydroflavonols. It can act on dihydrokaempferol to produce
CC       kaempferol, on dihydroquercetin to produce quercitin and on
CC       dihydromyricetin to produce myricetin (By similarity).
CC   -!- CATALYTIC ACTIVITY: A dihydroflavonol + 2-oxoglutarate + O(2) = a
CC       flavonol + succinate + CO(2) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: A flavanone + 2-oxoglutarate + O(2) = a
CC       dihydroflavonol + succinate + CO(2).
CC   -!- COFACTOR: Binds 1 iron ion per subunit (By similarity).
CC   -!- COFACTOR: Binds 1 ascorbate molecule per subunit (By similarity).
CC   -!- PATHWAY: Secondary metabolite biosynthesis; flavonoid
CC       biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the iron/ascorbate-dependent oxidoreductase
CC       family.
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DR   EMBL; AF240764; AAF64168.1; -; mRNA.
DR   HSSP; Q96323; 1GP6.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0045431; F:flavonol synthase activity; IEA:EC.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0031418; F:L-ascorbic acid binding; IEA:UniProtKB-KW.
DR   GO; GO:0045486; F:naringenin 3-dioxygenase activity; IEA:EC.
DR   GO; GO:0016702; F:oxidoreductase activity, acting on single d...; IEA:UniProtKB-KW.
DR   GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR005123; 2OG-FeII_Oase.
DR   Pfam; PF03171; 2OG-FeII_Oxy; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Dioxygenase; Flavonoid biosynthesis; Iron; Metal-binding;
KW   Oxidoreductase; Vitamin C.
FT   CHAIN         1    334       Flavonol synthase/flavanone 3-
FT                                hydroxylase.
FT                                /FTId=PRO_0000067293.
FT   METAL       220    220       Iron (By similarity).
FT   METAL       222    222       Iron (By similarity).
FT   METAL       276    276       Iron (By similarity).
SQ   SEQUENCE   334 AA;  38081 MW;  4097830ABE2534E2 CRC64;
     MEVQRVQEIA SLSKVIDTIP AEYIRSENEQ PVISTVHGVV LEVPVIDLSD SDEKKIVGLV
     SEASKEWGIF QVVNHGIPNE VIRKLQEVGK HFFELPQEEK ELIAKPEGSQ SIEGYGTRLQ
     KEVDGKKGWV DHLFHKIWPP SAINYQFWPK NPPAYREANE EYAKRLQLVV DNLFKYLSLG
     LDLEPNSFKD GAGGDDLVYL MKINYYPPCP RPDLALGVAH TDMSAITVLV PNEVPGLQVY
     KDGHWYDCKY IPNALIVHIG DQVEIMSNGK YKSVYHRTTV NKEKTRMSWP VFLEPPPDHE
     VGPIPKLVNE ENPAKFKTKK YKDYAYCKLN KLPQ
//