[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Gill; Farmerie W.G., Holland K.P., Catches J.S., Morrison-Shetlar A.I., Claiborne J.B. III, Edwards S.L., Evans D.H.; Submitted (MAY-2002) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Produces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body (By similarity).
CATALYTIC ACTIVITY: L-arginine + n NADPH + m O₂ = citrulline + nitric oxide + n NADP⁺.
COFACTOR: Heme group (By similarity).
COFACTOR: Binds 1 FAD (By similarity).
COFACTOR: Binds 1 FMN (By similarity).
SIMILARITY: Belongs to the NOS family.
SIMILARITY: Contains 1 flavodoxin-like domain.

Copyright

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Cross-references

Sequence databases

EMBL

AF232227; AAF36405.2; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P29477; 1NOC. [HSSP ENTRY / PDB]

ModBase

Q9I9M2.

Family and domain databases

InterPro

IPR001094; Flavdoxin_like.
IPR008254; Flavodoxin/NO_synth.
IPR004030; NO_synthase_oxygenase_reg.
IPR001478; PDZ.
Graphical view of domain structure.

Pfam

PF00258; Flavodoxin_1; 1.
PF02898; NO_synthase; 1.
Pfam graphical view of domain structure.

PRINTS

PR00369; FLAVODOXIN.

PROSITE

PS50902; FLAVODOXIN_LIKE; 1.
PS60001; NOS; PARTIAL.
PS50106; PDZ; PARTIAL.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q9I9M2.

Phylogenomic databases

HOVERGEN

Q9I9M2; -.

Other

ProtoNet

Q9I9M2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calmodulin-binding; FAD; Flavoprotein; FMN; Heme; Iron; Metal-binding; NADP; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >212`	`>212`	`Nitric oxide synthase.`	`PRO_0000170948`
`DOMAIN`	`60 >212`	`>153`	`Flavodoxin-like.`
`NP_BIND`	`186 >212`	`>27`	`FMN (By similarity).`
`REGION`	`30 50`	`21`	`Calmodulin-binding (Potential).`
`NON_TER`	`1 1`
`NON_TER`	`212 212`

Sequence information

Length: 212 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 23651 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: 3A6B115A679E6CFF [This is a checksum on the sequence]

        10         20         30         40         50         60 
LNYQLSPSFE YQSDPWFTHV WKGVNGTPTK KRAIGFKKLA KAVKFSTKLM GQAMAKRVKA 

        70         80         90        100        110        120 
TILYATETGK SQVYAKTLCE IFKHAFDAKV MSMDEYDIVH LEHEALVLVV TSTFGNGDPP 

       130        140        150        160        170        180 
ENGEKFGSAL MEIRHPSSNS AERKSYKVRF NSVSSYSDSR KSSSDEPEHK DNFESTGPLA 

       190        200        210 
NVRFSAFGLG SRAYPHFCAF ARAVDTLLEE LG

Q9I9M2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools