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UniProtKB/Swiss-Prot entry Q9FH76


[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name ABAH3_ARATH
Primary accession number Q9FH76
Secondary accession number Q3E8G7
Integrated into Swiss-Prot on May 29, 2007
Sequence was last modified on March 1, 2001 (Sequence version 1)
Annotations were last modified on    September 2, 2008 (Entry version 52)
Name and origin of the protein
Protein name Abscisic acid 8'-hydroxylase 3
Synonyms ABA 8'-hydroxylase 3
EC 1.14.13.93
Cytochrome P450 707A3
Gene name
Name: CYP707A3
OrderedLocusNames: At5g45340
ORFNames: K9E15.12
From
Arabidopsis thaliana (Mouse-ear cress) [TaxID: 3702] 
Taxonomy Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons; rosids; eurosids II; Brassicales; Brassicaceae; Arabidopsis.
Protein existence 1: Evidence at protein level;
References
[1]
NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY, AND INDUCTION.
DOI=10.1104/pp.103.037614; PubMed=15064374 [NCBI, ExPASy, EBI, Israel, Japan]
Saito S., Hirai N., Matsumoto C., Ohigashi H., Ohta D., Sakata K., Mizutani M.;
"Arabidopsis CYP707As encode (+)-abscisic acid 8'-hydroxylase, a key enzyme in the oxidative catabolism of abscisic acid.";
Plant Physiol. 134:1439-1449(2004).
[2]
NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=cv. Columbia;
DOI=10.1093/dnares/7.1.31; PubMed=10718197 [NCBI, ExPASy, EBI, Israel, Japan]
Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Kotani H., Tabata S.;
"Structural analysis of Arabidopsis thaliana chromosome 5. X. Sequence features of the regions of 3,076,755 bp covered by sixty P1 and TAC clones.";
DNA Res. 7:31-63(2000).
[3]
NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
STRAIN=cv. Columbia;
DOI=10.1126/science.1088305; PubMed=14593172 [NCBI, ExPASy, EBI, Israel, Japan]
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.;
"Empirical analysis of transcriptional activity in the Arabidopsis genome.";
Science 302:842-846(2003).
[4]
IDENTIFICATION, FUNCTION, MUTAGENESIS OF CYS-411, ENZYME REGULATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, AND INDUCTION.
DOI=10.1038/sj.emboj.7600121; PubMed=15044947 [NCBI, ExPASy, EBI, Israel, Japan]
Kushiro T., Okamoto M., Nakabayashi K., Yamagishi K., Kitamura S., Asami T., Hirai N., Koshiba T., Kamiya Y., Nambara E.;
"The Arabidopsis cytochrome P450 CYP707A encodes ABA 8'-hydroxylases: key enzymes in ABA catabolism.";
EMBO J. 23:1647-1656(2004).
[5]
FUNCTION, AND INDUCTION.
DOI=10.1111/j.1365-313X.2006.02683.x; PubMed=16623881 [NCBI, ExPASy, EBI, Israel, Japan]
Umezawa T., Okamoto M., Kushiro T., Nambara E., Oono Y., Seki M., Kobayashi M., Koshiba T., Kamiya Y., Shinozaki K.;
"CYP707A3, a major ABA 8'-hydroxylase involved in dehydration and rehydration response in Arabidopsis thaliana.";
Plant J. 46:171-182(2006).
[6]
INDUCTION BY PHYTOCHROME B.
DOI=10.1111/j.1365-313X.2006.02881.x; PubMed=17010113 [NCBI, ExPASy, EBI, Israel, Japan]
Seo M., Hanada A., Kuwahara A., Endo A., Okamoto M., Yamauchi Y., North H., Marion-Poll A., Sun T.P., Koshiba T., Kamiya Y., Yamaguchi S., Nambara E.;
"Regulation of hormone metabolism in Arabidopsis seeds: phytochrome regulation of abscisic acid metabolism and abscisic acid regulation of gibberellin metabolism.";
Plant J. 48:354-366(2006).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AB122150; BAD16630.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AB020744; BAB10255.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY065065; AAL57698.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AY102136; AAM26703.1; -; mRNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_199347.2; -.
NP_851136.1; -.
UniGene At.9625
3D structure databases
ModBase Q9FH76.
Enzyme and pathway databases
BioCyc MetaCyc:AT5G45340-MON; -.
Organism-specific databases
GeneFarm 1253; 94.
TAIR At5g45340; -.
Family and domain databases
InterPro IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.
Gene3D G3DSA:1.10.630.10; Cyt_P450; 1.
PANTHER PTHR19383; Cyt_P450; 1.
Pfam PF00067; p450; 1.
Pfam graphical view of domain structure.
PRINTS PR00463; EP450I.
PR00385; P450.
PROSITE PS00086; CYTOCHROME_P450; 1.
BLOCKS Q9FH76.
Genome annotation databases
GeneID 834570; -.
GenomeReviews BA000015_GR; AT5G45340.
KEGG ath:AT5G45340; -.
NMPDR fig|3702.1.peg.26309; -.
Other
ProtoNet Q9FH76.
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Alternative splicing; Complete proteome; Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP; Oxidoreductase; Stress response; Transmembrane.
Features
SEVIEWER logo Feature table viewer FT aligner logo Feature aligner
KeyFrom   To Length Description FTId
CHAIN   1   463  463     Abscisic acid 8'-hydroxylase 3. PRO_0000288641
TRANSMEM   6    26  21     Potential. 
METAL   411   411        Iron (heme axial ligand) (By similarity). 
VAR_SEQ   434   447        WSIVGPSDGIQYGP -> LVHLQNDNSPFGN (in isoform 2). VSP_025739
VAR_SEQ   448   463        Missing (in isoform 2). VSP_025740
MUTAGEN   411   411        C->A: Loss of activity. 
Sequence information
Length: 463 AA [This is the length of the unprocessed precursor] Molecular weight: 52367 Da [This is the MW of the unprocessed precursor] CRC64: CCD17293F553F812 [This is a checksum on the sequence]
        10         20         30         40         50         60 
MDFSGLFLTL SAAALFLCLL RFIAGVRRSS STKLPLPPGT MGYPYVGETF QLYSQDPNVF 

        70         80         90        100        110        120 
FAAKQRRYGS VFKTHVLGCP CVMISSPEAA KFVLVTKSHL FKPTFPASKE RMLGKQAIFF 

       130        140        150        160        170        180 
HQGDYHSKLR KLVLRAFMPD AIRNMVPHIE SIAQESLNSW DGTQLNTYQE MKTYTFNVAL 

       190        200        210        220        230        240 
ISILGKDEVY YREDLKRCYY ILEKGYNSMP INLPGTLFHK AMKARKELAQ ILANILSKRR 

       250        260        270        280        290        300 
QNPSSHTDLL GSFMEDKAGL TDEQIADNII GVIFAARDTT ASVLTWILKY LADNPTVLEA 

       310        320        330        340        350        360 
VTEEQMAIRK DKKEGESLTW EDTKKMPLTY RVIQETLRAA TILSFTFREA VEDVEYEGYL 

       370        380        390        400        410        420 
IPKGWKVLPL FRNIHHNADI FSDPGKFDPS RFEVAPKPNT FMPFGSGIHS CPGNELAKLE 

       430        440        450        460 
ISVLIHHLTT KYRWSIVGPS DGIQYGPFAL PQNGLPIALE RKP 

Q9FH76 in FASTA format

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