ID   LDH3_LACLA              Reviewed;         323 AA.
AC   Q9CGG8;
DT   21-JUN-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   04-NOV-2008, entry version 46.
DE   RecName: Full=L-lactate dehydrogenase 3;
DE            Short=L-LDH 3;
DE            EC=1.1.1.27;
GN   Name=ldhX; OrderedLocusNames=LL1128; ORFNames=L142159;
OS   Lactococcus lactis subsp. lactis (Streptococcus lactis).
OC   Bacteria; Firmicutes; Lactobacillales; Streptococcaceae; Lactococcus.
OX   NCBI_TaxID=1360;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=IL1403;
RX   MEDLINE=21235186; PubMed=11337471; DOI=10.1101/gr.GR-1697R;
RA   Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K.,
RA   Weissenbach J., Ehrlich S.D., Sorokin A.;
RT   "The complete genome sequence of the lactic acid bacterium Lactococcus
RT   lactis ssp. lactis IL1403.";
RL   Genome Res. 11:731-753(2001).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; AE006345; AAK05226.1; -; Genomic_DNA.
DR   PIR; H86765; H86765.
DR   RefSeq; NP_267284.1; -.
DR   HSSP; P00344; 2LDB.
DR   GeneID; 1114768; -.
DR   GenomeReviews; AE005176_GR; LL1128.
DR   KEGG; lla:L142159; -.
DR   NMPDR; fig|272623.1.peg.1160; -.
DR   HOGENOM; Q9CGG8; -.
DR   BioCyc; LLAC272623:L142159-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00488; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; FALSE_NEG.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    323       L-lactate dehydrogenase 3.
FT                                /FTId=PRO_0000168353.
FT   NP_BIND      14     42       NAD (By similarity).
FT   ACT_SITE    182    182       Proton acceptor (By similarity).
FT   BINDING      95     95       Substrate (By similarity).
FT   BINDING     127    127       NAD or substrate (By similarity).
FT   BINDING     158    158       Substrate (By similarity).
FT   BINDING     236    236       Substrate (By similarity).
SQ   SEQUENCE   323 AA;  35271 MW;  87CCB4EA290A15DD CRC64;
     MKINNKKVVI VGAGAVGSTY AHNLVVDDLA DEIAIINTNK SKASANSLDL LHALPYLNAA
     PKNIYAADYS DVSDADIVVL SANAPSATFG KNPDRLQLLE NKVEMIRDIT RKTMDAGFDG
     IFLVASNPVD VLAQVVAEVS GLPKHRVIGT GTLLETSRMR QIVAEKLQIN PKSIHGYVLA
     EHGKSSFAAW SNVTVGAIPL TTWLKKYPNP EFPTFDEIDQ EIREVGLDIF MQKGNTSYGI
     AASLARLTRA IFRNESVILP VSAYLTGEYG QFDLYTGSPA IIDRTGVRAV LELELTQEEQ
     EKFKASTVLL KENFDSIKEK CTL
//