[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. STRAIN=IL1403; DOI=10.1101/gr.GR-1697R; PubMed=11337471 [NCBI, ExPASy, EBI, Israel, Japan] Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.; "The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403."; Genome Res. 11:731-753(2001).
[2]	NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-253. STRAIN=210; PubMed=9212417 [NCBI, ExPASy, EBI, Israel, Japan] Erlandson K., Batt C.A.; "Strain-specific differentiation of lactococci in mixed starter culture populations using randomly amplified polymorphic DNA-derived probes."; Appl. Environ. Microbiol. 63:2702-2707(1997).

Comments

CATALYTIC ACTIVITY: sn-glycerol 3-phosphate + O₂ = glycerone phosphate + H₂O₂.
COFACTOR: FAD (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the FAD-dependent glycerol-3-phosphate dehydrogenase family.
CAUTION: As L.lactis is unable to produce acid from glycerol, the significance and/or function of the glpO gene in this organism is at present unknown.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE006356; AAK05343.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF001829; AAB63270.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

E86780; E86780.

RefSeq

NP_267401.1; -.

3D structure databases

ModBase

Q9CG65.

Enzyme and pathway databases

BioCyc

LLAC272623:L0013-MON; -.

Ontologies

GO:0004369;	Molecular function: glycerol-3-phosphate oxidase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR000447; FAD-dep_Gly3P_DHase.
IPR006076; FAD-dep_OxRdtase.
Graphical view of domain structure.

Pfam

PF01266; DAO; 1.
Pfam graphical view of domain structure.

PRINTS

PR01001; FADG3PDH.

PROSITE

PS00977; FAD_G3PDH_1; 1.
PS00978; FAD_G3PDH_2; FALSE_NEG.

Genome annotation databases

1114894; -.

AE005176_GR; LL1245.

lla:L0013; -.

fig|272623.1.peg.1280; -.

Phylogenomic databases

HOGENOM

Q9CG65; -.

Genome annotation databases

CMR

Q9CG65; LL1245.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; FAD; Flavoprotein; Glycerol metabolism; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 609`	`609`	`Alpha-glycerophosphate oxidase.`	`PRO_0000126109`
`NP_BIND`	`21 49`	`29`	`FAD (Potential).`
`CONFLICT`	`152 152`		`R -> H (in Ref. 2; AAB63270).`
`CONFLICT`	`253 253`		`K -> R (in Ref. 2; AAB63270).`

Sequence information

Length: 609 AA [This is the length of the unprocessed precursor]

Molecular weight: 67310 Da [This is the MW of the unprocessed precursor]

CRC64: E7A31EA7A2A89236 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAFSKKTRQE AITKIQNEEM DLLVIGGGIT GAGLTLQAAA AGMKVAVLEM QDFSEGTSSR 

        70         80         90        100        110        120 
STKLVHGGIR YLKNFDVEVV SDTVSERAVV QGIAPHIPKP DPMLLPIYDD EGKTTFDMFS 

       130        140        150        160        170        180 
VKIAMDLYDR LAGVDEDSPY ANYTISPEEV LRREPLIKKK GLQGAGVYLD YRNNDARLVI 

       190        200        210        220        230        240 
DNIKKAVEDG AQAISKMKVI DFIYTDGQIS GIRARDLLTD QVIEVKAKLV INTSGPWVDK 

       250        260        270        280        290        300 
IRYLNFTRPI VPKMRPTKGV HLVVDAAKLP VPQPTYFDTG KHDKRMVFAI PRENKTYFGT 

       310        320        330        340        350        360 
TDTDYHGDFT DPKVTQEDVD YLLDVINFRY PEANITINDI EASWAGLRPL LGGNSGSDYN 

       370        380        390        400        410        420 
GGDNGAVSET SFNAVVEAVL RYKNKTATKA EVEHLLNNME SSLSEKGDAP SSVSRGSSLE 

       430        440        450        460        470        480 
RESDGLITLA GGKITDYRKM AAGAMELICQ LLEEDFGLKY EPIDSKKYQI SGGEFDPTKV 

       490        500        510        520        530        540 
EEVVAENMKV GVAAGLTEEE AKYIADFYGM NALQVFAYAS EMEAYEGLSL AESARLCYAL 

       550        560        570        580        590        600 
EDEMILTPVD YLLRRTNHIL FMREGVDAIK VHVVNAMADD LGWSAEEKAE QEKALEEALR 


ESDLSDLKK

Q9CG65 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools