NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=IL1403;
DOI=10.1101/gr.GR-1697R; PubMed=11337471 [NCBI, ExPASy, EBI, Israel, Japan]
Bolotin A., Wincker P., Mauger S., Jaillon O., Malarme K., Weissenbach J., Ehrlich S.D., Sorokin A.;
"The complete genome sequence of the lactic acid bacterium Lactococcus lactis ssp. lactis IL1403.";
Genome Res. 11:731-753(2001).

Comments

CATALYTIC ACTIVITY: (S)-dihydroorotate + O₂ = orotate + H₂O₂.
COFACTOR: Binds 1 FMN per subunit (By similarity).
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6 .
SUBUNIT: Heterotetramer of 2 pyrK and 2 pyrD subunits (By similarity).
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE006366; AAK05444.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

B86793; B86793.

RefSeq

NP_267502.1; -.

3D structure databases

HSSP

P54322; 1EP2. [HSSP ENTRY / PDB]

SMR

Q9CFW8; 1-311.

ModBase

Q9CFW8.

Enzyme and pathway databases

BioCyc

LLAC272623:L182555-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0004158;	Molecular function: dihydroorotate oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00224; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR012135; DHO_DHase_1_2.
IPR005720; DHO_DHase_1_core.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000164; DHO_oxidase; 1.

TIGRFAMs

TIGR01037; pyrD_sub1_fam; 1.

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.

BLOCKS

Q9CFW8.

Genome annotation databases

1114996; -.

AE005176_GR; LL1346.

lla:L182555; -.

fig|272623.1.peg.1381; -.

Phylogenomic databases

HOGENOM

Q9CFW8; -.

Genome annotation databases

CMR

Q9CFW8; LL1346.

Other

ProtoNet

Q9CFW8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; Flavoprotein; FMN; Oxidoreductase; Pyrimidine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 311`	`311`	`Dihydroorotate dehydrogenase B, catalytic subunit.`	`PRO_0000148395`
`ACT_SITE`	`135 135`		`Nucleophile (By similarity).`

Sequence information

Length: 311 AA [This is the length of the unprocessed precursor]

Molecular weight: 32916 Da [This is the MW of the unprocessed precursor]

CRC64: 6FF419606B2E733C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTENNRLSVK LPGLDLKNPI IPASGCFGFG EEYAKYYDLN KLGSIMVKAT TLHPRFGNPT 

        70         80         90        100        110        120 
PRVAETASGM LNAIGLQNPG LEVIMAEKLP WLNENFPDLP IIANVAGSEE DDYVAVCAKI 

       130        140        150        160        170        180 
GDAPNVKVIE LNISCPNVKH GGQAFGTDPD VAAALVKACK AVSKVPLYVK LSPNVTDIVP 

       190        200        210        220        230        240 
IAKAVEAAGA DGLTMINTLM GVRFDLKTRK PVLANITGGL SGPAIKPVAL KLIHQVAQVV 

       250        260        270        280        290        300 
DIPIIGMGGV ESAQDVLEMY MAGASAVAVG TANFADPFVC PKIIEKLPEV MDQYGIDSLE 

       310 
NLIQEVKNSK K

Q9CFW8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools