ID   G3P2_RHIRA              Reviewed;         338 AA.
AC   Q96UF2;
DT   09-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2001, sequence version 1.
DT   25-NOV-2008, entry version 39.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase 2;
DE            Short=GAPDH 2;
DE            EC=1.2.1.12;
GN   Name=GPD2;
OS   Rhizomucor racemosus (Mucor circinelloides f. lusitanicus).
OC   Eukaryota; Fungi; Fungi incertae sedis; Basal fungal lineages;
OC   Mucoromycotina; Mucorales; Mucoraceae; Mucor.
OX   NCBI_TaxID=4841;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 90680 / R7B;
RX   PubMed=11860262; DOI=10.1006/fgbi.2001.1313;
RA   Wolff A.M., Arnau J.;
RT   "Cloning of glyceraldehyde-3-phosphate dehydrogenase-encoding genes in
RT   Mucor circinelloides (Syn. racemosus) and use of the gpd1 promoter for
RT   recombinant protein production.";
RL   Fungal Genet. Biol. 35:21-29(2002).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH.
CC   -!- PATHWAY: Carbohydrate degradation; glycolysis; pyruvate from D-
CC       glyceraldehyde 3-phosphate: step 1/5.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; AJ293013; CAC37404.1; -; Genomic_DNA.
DR   HSSP; P56649; 1DSS.
DR   SMR; Q96UF2; 4-331.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD binding; IEA:InterPro.
DR   GO; GO:0006096; P:glycolysis; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DHase.
DR   InterPro; IPR006424; Glyceraldehyde-3-P_DHase_1.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PIRSF; PIRSF000149; GAP_DH; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   TIGRFAMs; TIGR01534; GAPDH-I; 1.
DR   PROSITE; PS00071; GAPDH; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Glycolysis; NAD; Oxidoreductase.
FT   CHAIN         1    338       Glyceraldehyde-3-phosphate dehydrogenase
FT                                2.
FT                                /FTId=PRO_0000145577.
FT   NP_BIND      12     13       NAD (By similarity).
FT   REGION      150    152       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      210    211       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE    151    151       Nucleophile (By similarity).
FT   BINDING      34     34       NAD (By similarity).
FT   BINDING      79     79       NAD; via carbonyl oxygen (By similarity).
FT   BINDING     181    181       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     233    233       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     315    315       NAD (By similarity).
FT   SITE        178    178       Activates thiol group during catalysis
FT                                (By similarity).
SQ   SEQUENCE   338 AA;  35864 MW;  DE0D19A20D797AEF CRC64;
     MVTQVGINGF GRIGRIVLRA SLSNPEVQVV AINDPFIPLE YMVYMFKYDS VHGRFQGTVE
     AKDGKLVVNG KEISVFSERD PAQIPWGSVE AAYVVESTGV FTSIDAASAH LQGGAKKVII
     SAPSGDAPMF VCGVNLEKYT SDLKVISNAS CTTNCLAPLA KVINDNFGIV EGLMTTVHAT
     TATQKTVDGP SNKDWRGGRG AGANIIPSST GAAKAVGKVI PELNGKLTGM AFRVPTPDVS
     VVDLTVRLEK GATYEEIKAV IKKASENELK GILGYTNDQV VSTDFVGDAQ SSIFDAAAGI
     ALNDKFVKLV SWYDNEFGYS NRVIDLLAYA AKVDAAAQ
//