NUCLEOTIDE SEQUENCE [MRNA].
TISSUE=Interrenal gland;
DOI=10.1016/S0378-1119(96)00734-2; PubMed=9073075 [NCBI, ExPASy, EBI, Israel, Japan]
Nunez S., Trant J.M.;
"Isolation of the putative cDNA encoding cholesterol side chain cleavage cytochrome P450 (CYP11A) of the southern stingray (Dasyatis americana).";
Gene 187:123-129(1997).

Comments

FUNCTION: Catalyzes the side-chain cleavage reaction of cholesterol to pregnenolone.
CATALYTIC ACTIVITY: Cholesterol + reduced adrenal ferredoxin + O₂ = pregnenolone + 4-methylpentanal + oxidized adrenal ferredoxin + H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Lipid metabolism; C21-steroid hormone metabolism .
SUBCELLULAR LOCATION: Mitochondrion membrane.
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

U63299; AAC60095.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P00189; 1SCC. [HSSP ENTRY / PDB]

ModBase

Q92045.

Ontologies

GO:0031966;	Cellular component: mitochondrial membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q92045.

Phylogenomic databases

HOVERGEN

Q92045; -.

Other

ProtoNet

Q92045.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cholesterol metabolism; Heme; Iron; Lipid metabolism; Membrane; Metal-binding; Mitochondrion; Monooxygenase; Oxidoreductase; Steroid metabolism; Steroidogenesis; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`<1 39`	`>39`	`Mitochondrion (By similarity).`
`CHAIN`	`40 514`	`475`	`Cytochrome P450 11A1, mitochondrial.`	`PRO_0000003592`
`METAL`	`461 461`		`Iron (heme axial ligand) (By similarity).`
`NON_TER`	`1 1`

Sequence information

Length: 514 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 59863 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: D0F03E25D5534FB2 [This is a checksum on the sequence]

        10         20         30         40         50         60 
SFRLSLSAST YAQRGSFTTP EHDFTLFPHR NHSVTSESRI PSEQTLKSLT DIPGNWRKNW 

        70         80         90        100        110        120 
LNVYYFWRSN GLNNAHQWML DNFNKYGPIY REKIAYYESI NIINPADAVI MNKSEGPFPK 

       130        140        150        160        170        180 
RIEMAPWVAY RDLRKENYGV QLLNGENWKR TRLILNNSIF AQSSIQRLVP LFNEVVLDFV 

       190        200        210        220        230        240 
SMVHKEVEKS RSDYWKTDLT NDLFKLALEV ICYILYGERL DLLQRKYNKA PQKFIDSIAT 

       250        260        270        280        290        300 
MFHSTPIMLY VPPSLLKSIN SKIWQQHVGS WDNIFEHADT YLKKAYRQFQ QGSKNEHAFP 

       310        320        330        340        350        360 
GVLTELLLQG ALPFEDIRAS IIDVMSGAID TTSTTVHWMM YELAKHPHIQ KNVRSEIMEA 

       370        380        390        400        410        420 
HQKTEGDPVK MLKSVPLLKC VVKETLRLYP VAISIQRYLN EDTVLQNYHI PAGTLVQLGL 

       430        440        450        460        470        480 
YAMGRNPKIF KNPEQYNPER WLKGEDTHFR HLGFGFGPRQ CIGRRIAETQ MVLLMIHMLQ 

       490        500        510 
NFKIETDPMT EVKSKFSLIL IPDKPINLKF TPIK

Q92045 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools