NUCLEOTIDE SEQUENCE [MRNA], AND INDUCTION.
STRAIN=SAG 211-8b;
DOI=10.1007/s00425-005-1501-0; PubMed=15830207 [NCBI, ExPASy, EBI, Israel, Japan]
Valverde F., Ortega J.M., Losada M., Serrano A.;
"Sugar-mediated transcriptional regulation of the Gap gene system and concerted photosystem II functional modulation in the microalga Scenedesmus vacuolatus.";
Planta 221:937-952(2005).

Comments

CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate + NADP⁺ = 3-phospho-D-glyceroyl phosphate + NADPH.
PATHWAY: Carbohydrate biosynthesis; Calvin cycle .
SUBUNIT: Homotetramer (By similarity).
SUBCELLULAR LOCATION: Plastid, chloroplast (By similarity).
INDUCTION: Repressed by addition of D-galactose, D-mannose, D-glucose, D-ribose or sucrose, but not by the addition of non-metabolizable sugar analogs.
MISCELLANEOUS: GAPDHB, the second subunit found in plants, is absent in algae.
MISCELLANEOUS: Algae contain three forms of GAPDH: two cytosolic forms which participate in glycolysis and one chloroplastic form which participates in photosynthesis. These three forms are encoded by distinct genes.
SIMILARITY: Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ252208; CAC81011.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

HSSP

P19866; 1NBO. [HSSP ENTRY / PDB]

SMR

Q8VXQ9; 1-314.

ModBase

Q8VXQ9.

Ontologies

GO:0047100;	Molecular function: glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR000173; GlycerAld_3-P_DHase.
IPR006424; Glyceraldehyde-3-P_DHase_1.
Graphical view of domain structure.

PANTHER

PTHR10836; GAP_DH; 1.

Pfam

PF02800; Gp_dh_C; 1.
PF00044; Gp_dh_N; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000149; GAP_DH; 1.

PRINTS

PR00078; G3PDHDRGNASE.

TIGRFAMs

TIGR01534; GAPDH-I; 1.

PS00071; GAPDH; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`<1 >314`	`>314`	`Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic.`	`PRO_0000292345`
`NP_BIND`	`5 6`	`2`	`NADP (By similarity).`
`REGION`	`147 149`	`3`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`REGION`	`206 207`	`2`	`Glyceraldehyde 3-phosphate binding (By similarity).`
`ACT_SITE`	`148 148`		`Nucleophile (By similarity).`
`BINDING`	`29 29`		`NADP (By similarity).`
`BINDING`	`74 74`		`NADP; via carbonyl oxygen (By similarity).`
`BINDING`	`178 178`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`193 193`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`229 229`		`Glyceraldehyde 3-phosphate (By similarity).`
`BINDING`	`311 311`		`NADP (By similarity).`
`SITE`	`175 175`	`1`	`Activates thiol group during catalysis (By similarity).`
`DISULFID`	`13 283`		`By similarity.`
`NON_TER`	`1 1`
`NON_TER`	`314 314`

Sequence information

Length: 314 AA [This is the length of the partial sequence of the unprocessed precursor]

Molecular weight: 33574 Da [This is the MW of the partial sequence of the unprocessed precursor]

CRC64: F0F3CA15BDC4DB77 [This is a checksum on the sequence]

        10         20         30         40         50         60 
NGFGRIGRNF LRCLETRQNS LLEVIAINDS GGVKQASHLL KYDSTLGKFD ADVKIVDDGH 

        70         80         90        100        110        120 
ISVNGKSIRV VSSRDPTKLP WGEMEIDLVI EGTGVFIDTP GASKHIEAGA KKVLITAPAK 

       130        140        150        160        170        180 
GSDIPTYVVG VNAHDYKHSD AIISNASCTT NCLAPFVKVL DEKFGIVKGT MTNTHSYTGD 

       190        200        210        220        230        240 
QRLLDASHRD LRRARAAALN IVPTTTGAAK AVALVLPKLK GKLNGIALRV PTPNVSVVDL 

       250        260        270        280        290        300 
VVQVEKKTFA EEINNAFKEA AAGSLNGVLA VSDEPLVSVD FRCTDVSSTI DSSLTMVMGA 

       310 
DMVKVVAWYD NEWG

Q8VXQ9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools