ID   COMX2_BACSU             Reviewed;          58 AA.
AC   Q8VL79;
DT   18-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   22-JUL-2008, entry version 22.
DE   RecName: Full=Competence pheromone;
DE   Flags: Precursor;
GN   Name=comX;
OS   Bacillus subtilis.
OC   Bacteria; Firmicutes; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=1423;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], POST-TRANSLATIONAL MODIFICATIONS,
RP   AND MASS SPECTROMETRY.
RC   STRAIN=168 / RO-A-4, 168 / RO-PP-2, W23 / DV3-D-2, and W23 / RO-E-2;
RX   MEDLINE=22063669; PubMed=12067344;
RA   Ansaldi M., Marolt D., Stebe T., Mandic-Mulec I., Dubnau D.;
RT   "Specific activation of the Bacillus quorum-sensing systems by
RT   isoprenylated pheromone variants.";
RL   Mol. Microbiol. 44:1561-1573(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=W23 / DV3-D-2;
RA   Sabotic J., Cepon U., Cadez P., Mandic Mulec I.;
RT   "Variability of Bacillus sp. quorum sensing system.";
RL   Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   ISOPRENYLATION AT TRP-56.
RX   PubMed=16407988; DOI=10.1038/nchembio709;
RA   Okada M., Sato I., Cho S.J., Iwata H., Nishio T., Dubnau D.,
RA   Sakagami Y.;
RT   "Structure of the Bacillus subtilis quorum-sensing peptide pheromone
RT   ComX.";
RL   Nat. Chem. Biol. 1:23-24(2005).
RN   [4]
RP   POLYMORPHISM IN COMX; COMQ AND COMP.
RX   PubMed=11133937; DOI=10.1128/JB.183.2.451-460.2001;
RA   Tortosa P., Logsdon L., Kraigher B., Itoh Y., Mandic-Mulec I.,
RA   Dubnau D.;
RT   "Specificity and genetic polymorphism of the Bacillus competence
RT   quorum-sensing system.";
RL   J. Bacteriol. 183:451-460(2001).
RN   [5]
RP   POLYMORPHISM, AND DETERMINANTS OF MODIFICATION SPECIFICITY.
RX   PubMed=14679219; DOI=10.1128/JB.186.1.15-21.2004;
RA   Ansaldi M., Dubnau D.;
RT   "Diversifying selection at the Bacillus quorum-sensing locus and
RT   determinants of modification specificity during synthesis of the ComX
RT   pheromone.";
RL   J. Bacteriol. 186:15-21(2004).
CC   -!- FUNCTION: Part of a major quorum-sensing system that regulates the
CC       development of genetic competence. Acts through the activation of
CC       the two-component regulatory system comP/comA composed of a sensor
CC       histidine kinase, comP, and a response regulator, comA, that
CC       regulates directly the transcription of over 20 genes. Transport
CC       through the membrane may involve spo0K. Under certain conditions
CC       plays a role in sporulation.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- PTM: Isoprenylation of Trp-56 is essential for activity. The type
CC       of isoprenyl derivative differs among the different pherotypes and
CC       depends on comX primary sequence. ComQ is required for the
CC       production of comX and may be responsible alone for its complete
CC       maturation.
CC   -!- MISCELLANEOUS: The DNA sequences encoding comQ, comX and the N-
CC       terminal two-thirds of comP show a striking polymorphism, which
CC       determines the specificity of the quorum-sensing system in the
CC       different pherotypes of Bacillus. In comX, the sole conserved
CC       residue is the modified tryptophan essential for the activity.
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DR   EMBL; AF456138; AAL67740.1; -; Genomic_DNA.
DR   EMBL; AF456131; AAL67719.1; -; Genomic_DNA.
DR   EMBL; AF456132; AAL67722.1; -; Genomic_DNA.
DR   EMBL; AF458695; AAL59650.1; -; Genomic_DNA.
DR   EMBL; DQ241785; ABB51994.1; -; Genomic_DNA.
DR   InterPro; IPR009233; Competence_ComX_Bacillus.
DR   Pfam; PF05952; ComX; 1.
PE   1: Evidence at protein level;
KW   Competence; Lipoprotein; Pheromone; Prenylation; Secreted;
KW   Sporulation.
FT   PROPEP        1     52
FT                                /FTId=PRO_0000233036.
FT   PEPTIDE      53     58       Competence pheromone.
FT                                /FTId=PRO_0000233037.
FT   LIPID        56     56       3'-geranyl-2',N2-cyclotryptophan; in
FT                                strain W23 / RO-E-2.
SQ   SEQUENCE   58 AA;  6684 MW;  BC6095F268EC1EFE CRC64;
     MKQDMIDYLM KNPQVLTKLE NGEASLIGIP DKLIPSIVDI FNKKMTLSKK CKGIFWEQ
//