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UniProtKB/Swiss-Prot entry Q8TXY4

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name MER_METKA
Primary accession number Q8TXY4
Secondary accession number Q49598
Integrated into Swiss-Prot on November 28, 2003
Sequence was last modified on January 23, 2007 (Sequence version 3)
Annotations were last modified on    November 4, 2008 (Entry version 44)
Name and origin of the protein
Protein name 5,10-methylenetetrahydromethanopterin reductase
Synonyms EC 1.5.99.11
Coenzyme F420-dependent N(5),N(10)-methylenetetrahydromethanopterin reductase
Methylene-H(4)MPT reductase
Gene name
Name: mer
OrderedLocusNames: MK0524
From
Methanopyrus kandleri [TaxID: 2320] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanopyri; Methanopyrales; Methanopyraceae; Methanopyrus.
Protein existence 1: Evidence at protein level;
References
[1]
 NUCLEOTIDE SEQUENCE [GENOMIC DNA].
PubMed=8522533 [NCBI, ExPASy, EBI, Israel, Japan]
Noelling J., Pihl T.D., Reeve J.N.;
"Cloning, sequencing, and growth phase-dependent transcription of the coenzyme F420-dependent N5,N10-methylenetetrahydromethanopterin reductase-encoding genes from Methanobacterium thermoautotrophicum delta H and Methanopyrus kandleri.";
J. Bacteriol. 177:7238-7244(1995).
[2]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
DOI=10.1073/pnas.032671499; PubMed=11930014 [NCBI, ExPASy, EBI, Israel, Japan]
Slesarev A.I., Mezhevaya K.V., Makarova K.S., Polushin N.N., Shcherbinina O.V., Shakhova V.V., Belova G.I., Aravind L., Natale D.A., Rogozin I.B., Tatusov R.L., Wolf Y.I., Stetter K.O., Malykh A.G., Koonin E.V., Kozyavkin S.A.;
"The complete genome of hyperthermophile Methanopyrus kandleri AV19 and monophyly of archaeal methanogens.";
Proc. Natl. Acad. Sci. U.S.A. 99:4644-4649(2002).
[3]
 PROTEIN SEQUENCE OF 2-27, AND CHARACTERIZATION.
STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
DOI=10.1007/BF00245355; PubMed=1953299 [NCBI, ExPASy, EBI, Israel, Japan]
Ma K., Linder D., Stetter K.O., Thauer R.K.;
"Purification and properties of N5,N10-methylenetetrahydromethanopterin reductase (coenzyme F420-dependent) from the extreme thermophile Methanopyrus kandleri.";
Arch. Microbiol. 155:593-600(1991).
[4]
 X-RAY CRYSTALLOGRAPHY (1.65 ANGSTROMS).
STRAIN=AV19 / DSM 6324 / JCM 9639 / NBRC 100938;
DOI=10.1006/jmbi.2000.3909; PubMed=10891279 [NCBI, ExPASy, EBI, Israel, Japan]
Shima S., Warkentin E., Grabarse W., Sordel M., Wicke M., Thauer R.K., Ermler U.;
"Structure of coenzyme F(420) dependent methylenetetrahydromethanopterin reductase from two methanogenic archaea.";
J. Mol. Biol. 300:935-950(2000).
Comments
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
U31567; AAA92085.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
AE010346; AAM01739.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_613809.1; -.
3D structure databases
PDB
1EZW; X-ray; 1.65 A; A=1-349.[ExPASy / RCSB / EBI]
PDBsum 1EZW; -.
ModBase Q8TXY4.
Enzyme and pathway databases
BioCyc MKAN190192:MK0524-MON; -.
Ontologies
GO
GO:0005737; Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0018537; Molecular function: coenzyme F420-dependent N5,N10-methenyltetrahydromethanopterin reductase activity (inferred from electronic annotation from HAMAP).
GO:0015948; Biological process: methanogenesis (inferred from electronic annotation from UniProtKB-KW).
GO:0055114; Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.
Family and domain databases
HAMAP MF_01091; -; 1.
PBIL [Tree]
InterPro IPR011251; Luciferase-like_bac.
IPR016048; Luciferase_mOase.
Graphical view of domain structure.
Gene3D G3DSA:3.20.20.30; Luciferase_like; 1.
Pfam PF00296; Bac_luciferase; 1.
Pfam graphical view of domain structure.
ProtoNet Q8TXY4.
Genome annotation databases
GeneID 1476625; -.
GenomeReviews AE009439_GR; MK0524.
KEGG mka:MK0524; -.
NMPDR fig|190192.1.peg.522; -.
Phylogenomic databases
HOGENOM Q8TXY4; -.
Genome annotation databases
CMR Q8TXY4; MK0524.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
3D-structure; Complete proteome; Cytoplasm; Direct protein sequencing; Methanogenesis; One-carbon metabolism; Oxidoreductase.
Features
SEVIEWER logo Feature table viewer
KeyFrom   To Length Description FTId
INIT_MET   1     1        Removed. 
CHAIN   2   349  348     5,10-methylenetetrahydromethanopterin reductase. PRO_0000084808
CONFLICT   21    21        H -> A (in Ref. 3; AA sequence). 
CONFLICT   162   162        N -> K (in Ref. 1; AAA92085). 
CONFLICT   191   191        D -> N (in Ref. 1; AAA92085). 
CONFLICT   344   344        I -> L (in Ref. 1; AAA92085). 
STRAND   4     9  6      
HELIX   15    27  13      
STRAND   32    35  4      
HELIX   44    53  10      
STRAND   56    65  10      
STRAND   67    70  4      
HELIX   72    85  14      
STRAND   92    94  3      
HELIX   99   105  7      
HELIX   126   141  16      
STRAND   151   154  4      
HELIX   167   169  3      
STRAND   172   175  4      
HELIX   179   188  10      
STRAND   190   195  6      
HELIX   199   215  17      
HELIX   220   222  3      
STRAND   223   228  6      
STRAND   231   233  3      
HELIX   237   253  17      
HELIX   257   262  6      
TURN   269   271  3      
HELIX   272   276  5      
TURN   277   280  4      
HELIX   282   288  7      
HELIX   291   297  7      
STRAND   299   302  4      
HELIX   303   315  13      
STRAND   320   323  4      
HELIX   331   341  11      
HELIX   343   345  3      
Sequence information
Length: 349 AA [This is the length of the unprocessed precursor] Molecular weight: 37501 Da [This is the MW of the unprocessed precursor] CRC64: 12AF28EDF65F26F0 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MAEVSFGIEL LPDDKPTKIA HLIKVAEDNG FEYAWICDHY NNYSYMGVLT LAAVITSKIK 

        70         80         90        100        110        120 
LGPGITNPYT RHPLITASNI ATLDWISGGR AIIGMGPGDK ATFDKMGLPF PCKIPIWNPE 

       130        140        150        160        170        180 
AEDEVGPATA IREVKEVIYQ YLEGGPVEYE GKYVKTGTAD VNARSIQGSD IPFYMGAQGP 

       190        200        210        220        230        240 
IMLKTAGEIA DGVLVNASNP KDFEVAVPKI EEGAKEAGRS LDEIDVAAYT CFSIDKDEDK 

       250        260        270        280        290        300 
AIEATKIVVA FIVMGSPDVV LERHGIDTEK AEQIAEAIGK GDFGTAIGLV DEDMIEAFSI 

       310        320        330        340 
AGDPDTVVDK IEELLKAGVT QVVVGSPIGP DKEKAIELVG QEVIPHFKE
Q8TXY4 in FASTA format

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