[1]	NUCLEOTIDE SEQUENCE [MRNA]. DOI=10.1042/0264-6021:3630297; PubMed=11931657 [NCBI, ExPASy, EBI, Israel, Japan] Lundell K.; "Cloning and expression of two novel pig liver and kidney fatty acid hydroxylases [cytochrome P450 (CYP)4A24 and CYP4A25]."; Biochem. J. 363:297-303(2002).

Comments

FUNCTION: Catalyzes the omega- and (omega-1)-hydroxylation of various fatty acids such as laurate and palmitate. Has no activity toward taurochenodeoxycholic acid.
CATALYTIC ACTIVITY: Octane + reduced rubredoxin + O₂ = 1-octanol + oxidized rubredoxin + H₂O.
COFACTOR: Heme group (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (Potential).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ318097; CAC85663.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

UniGene

Ssc.49966

3D structure databases

HSSP

P00179; 1DT6. [HSSP ENTRY / PDB]

ModBase

Q8SPK0.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002401; Cyt_P450_E_grp-I.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00463; EP450I.
PR00385; P450.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q8SPK0.

Phylogenomic databases

HOVERGEN

Q8SPK0; -.

Other

ProtoNet

Q8SPK0.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Monooxygenase; Oxidoreductase; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 504`	`504`	`Cytochrome P450 4A25.`	`PRO_0000280744`
`TRANSMEM`	`6 26`	`21`	`Potential.`
`TRANSMEM`	`110 130`	`21`	`Potential.`
`METAL`	`451 451`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 504 AA [This is the length of the unprocessed precursor]

Molecular weight: 57324 Da [This is the MW of the unprocessed precursor]

CRC64: C6FD45CA1EDA97DF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTVPALASAS GLLQVASLLG LLLLLLKAAQ LYLRRQWLLK ALQQFPSPPS HWLYGHSREF 

        70         80         90        100        110        120 
QEESELQPLL KRVEKYPSAC ARWLWGTRAM VLVYDPDYMK VVLARSEPKA PVLYRLLIPW 

       130        140        150        160        170        180 
IGCGLLLLNG QTWFQRRRML TPAFHYDILK PYVGLMAKSV QVMLDKWEQL VAQDPRLEIV 

       190        200        210        220        230        240 
GPVSLMTLDT IMKCAFSHQG SAQTDGDSHS YIQAIWDLKN LFSIRTKSAF LQNDIIYRLS 

       250        260        270        280        290        300 
PEGRKNHRAA RIAHQHTDRV IQLRKAQLQK QGEMENVRKK RHLDFLDILL LARMEKGNSL 

       310        320        330        340        350        360 
SDTDLRAEVD TFMFEGHDTT ASGISWILYA LASHPEHQQR CREEIQGLLG DGTSITWDHL 

       370        380        390        400        410        420 
DQMPYTTMCI KEALRLYPPV PGVSRELSKP ITFPDGRSLP AGIILSLSVY SLHHNPQVWP 

       430        440        450        460        470        480 
NPEEFDPSRF APGSARHSHA FMPFSGGSRN CIGKQFAMNE MKVAVALTLL RFELAPDPSR 

       490        500 
KPTVIPEVVL HSKNGIHLKL RKLP

Q8SPK0 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools