NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
PubMed=12125824 [NCBI, ExPASy, EBI, Israel, Japan]
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.;
"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea.";
J. Mol. Microbiol. Biotechnol. 4:453-461(2002).

Comments

FUNCTION: Catalyzes the reversible reduction of methenyl-H(4)MPT(+) to methylene-H(4)MPT (By similarity).
CATALYTIC ACTIVITY: 5,10-methylenetetrahydromethanopterin + coenzyme F420 = 5,10-methenyltetrahydromethanopterin + reduced coenzyme F420.
PATHWAY: One-carbon metabolism; methanogenesis from carbone dioxide; 5,10-methylene-H(4)MPT from 5,10-methenyl-H(4)MPT (cofactor F420 route): step 1/1 .
SIMILARITY: Belongs to the MTD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE008384; AAM30804.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_633132.1; -.

3D structure databases

ModBase

Q8PXV8.

Enzyme and pathway databases

BioCyc

MMAZ192952:MM1108-MON; -.

Ontologies

GO:0030268;	Molecular function: methylenetetrahydromethanopterin dehydrogenase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00058; -; 1.
PBIL [Tree]

InterPro

IPR002844; Methylene_DHase.
Graphical view of domain structure.

Pfam

PF01993; MTD; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF005627; MTD; 1.

BLOCKS

Q8PXV8.

Genome annotation databases

GeneID

1479450; -.

GenomeReviews

AE008384_GR; MM_1108.

KEGG

mma:MM_1108; -.

NMPDR

fig|192952.1.peg.1108; -.

Phylogenomic databases

HOGENOM

Q8PXV8; -.

Genome annotation databases

CMR

Q8PXV8; MM_1108.

Other

ProtoNet

Q8PXV8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Methanogenesis; One-carbon metabolism; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 279`	`279`		`F420-dependent methylenetetrahydromethanopterin dehydrogenase.`	`PRO_0000075040`

Sequence information

Length: 279 AA [This is the length of the unprocessed precursor]

Molecular weight: 30240 Da [This is the MW of the unprocessed precursor]

CRC64: F52747A8B98723DE [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKMVNIGFI KMGNLGMSQV INLIQDEIAA REGITVRVFG TGAKMAPADA ADTESFKQWN 

        70         80         90        100        110        120 
ADFVVIISPN AAAPGPTAAR EIWKDVPCII VSDGPTKKEA REAFEQEGFG YIILPVDPLI 

       130        140        150        160        170        180 
GAKREFLDSV EMASFNADAM KVLSICGVVR LIQEELDKVT EQVASGKSGK DLELPHIFAK 

       190        200        210        220        230        240 
PEKCVEHAGF ANPYAKSKAL AALHMAEKVA QVNFPACFML KEIEQVCLTA AAGHEIMGAA 

       250        260        270 
AQLANQAREI EKSNDTVSRM PHAKNGAVLK KVRLYEKPE

Q8PXV8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools