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UniProtKB/Swiss-Prot entry Q8PXH2

[Entry info] [Name and origin] [References] [Comments] [Cross-references] [Keywords] [Features] [Sequence] [Tools]

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Entry information
Entry name DP2L_METMA
Primary accession number Q8PXH2
Secondary accession numbers None
Integrated into Swiss-Prot on October 19, 2002
Sequence was last modified on October 19, 2002 (Sequence version 1)
Annotations were last modified on    July 22, 2008 (Entry version 35)
Name and origin of the protein
Protein name DNA polymerase II large subunit
Synonyms Pol II
EC 2.7.7.7
Gene name
Name: polC
OrderedLocusNames: MM_1246
From
Methanosarcina mazei (Methanosarcina frisia) [TaxID: 2209] [HAMAP proteome]
Taxonomy Archaea; Euryarchaeota; Methanomicrobia; Methanosarcinales; Methanosarcinaceae; Methanosarcina.
Protein existence 3: Inferred from homology;
References
[1]
 NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC BAA-159 / DSM 3647 / Goe1 / Go1 / JCM 11883 / OCM 88;
PubMed=12125824 [NCBI, ExPASy, EBI, Israel, Japan]
Deppenmeier U., Johann A., Hartsch T., Merkl R., Schmitz R.A., Martinez-Arias R., Henne A., Wiezer A., Baeumer S., Jacobi C., Brueggemann H., Lienard T., Christmann A., Boemecke M., Steckel S., Bhattacharyya A., Lykidis A., Overbeek R., Klenk H.-P., Gunsalus R.P., Fritz H.-J., Gottschalk G.;
"The genome of Methanosarcina mazei: evidence for lateral gene transfer between Bacteria and Archaea.";
J. Mol. Microbiol. Biotechnol. 4:453-461(2002).
Comments
  • FUNCTION: Possesses two activities: a DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Has a template-primer preference which is characteristic of a replicative DNA polymerase (By similarity).
  • CATALYTIC ACTIVITY: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1).
  • CATALYTIC ACTIVITY: Degradation of single-stranded DNA. It acts progressively in a 3'- to 5'-direction, releasing nucleoside 5'-phosphates.
  • SUBUNIT: Heterodimer of a large subunit and a small subunit (By similarity).
  • SIMILARITY: Belongs to the archaeal DNA polymerase II family.
Copyright
Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.
Cross-references
Sequence databases
EMBL
AE008384; AAM30942.1; -; Genomic_DNA.[EMBL / GenBank / DDBJ] [CoDingSequence]
RefSeq NP_633270.1; -.
3D structure databases
ModBase Q8PXH2.
Enzyme and pathway databases
BioCyc MMAZ192952:MM1246-MON; -.
Ontologies
GO
GO:0008408; Molecular function: 3'-5' exonuclease activity (inferred from electronic annotation from HAMAP).
GO:0003677; Molecular function: DNA binding (inferred from electronic annotation from HAMAP).
GO:0003887; Molecular function: DNA-directed DNA polymerase activity (inferred from electronic annotation from HAMAP).
GO:0000738; Biological process: DNA catabolic process, exonucleolytic (inferred from electronic annotation from HAMAP).
GO:0006261; Biological process: DNA-dependent DNA replication (inferred from electronic annotation from HAMAP).
QuickGo view.
Family and domain databases
HAMAP MF_00324; -; 1.
PBIL [Tree]
InterPro IPR004475; PolC_DP2.
IPR016033; PolC_DP2_N.
Graphical view of domain structure.
Pfam PF03833; PolC_DP2; 1.
Pfam graphical view of domain structure.
PIRSF PIRSF016275; PolC_DP2; 1.
TIGRFAMs TIGR00354; polC; 1.
ProtoNet Q8PXH2.
Genome annotation databases
GeneID 1479588; -.
GenomeReviews AE008384_GR; MM_1246.
KEGG mma:MM_1246; -.
NMPDR fig|192952.1.peg.1246; -.
Phylogenomic databases
HOGENOM Q8PXH2; -.
Genome annotation databases
CMR Q8PXH2; MM_1246.
Other
UniRef View cluster of proteins with at least 50% / 90% / 100% identity.
Keywords
Complete proteome; DNA replication; DNA-binding; DNA-directed DNA polymerase; Exonuclease; Hydrolase; Multifunctional enzyme; Nuclease; Nucleotidyltransferase; Transferase.
Features
SEVIEWER logo Feature table viewer
KeyFrom To Length Description FTId
CHAIN   1   1152  1152     DNA polymerase II large subunit. PRO_0000152577
Sequence information
Length: 1152 AA [This is the length of the unprocessed precursor] Molecular weight: 128786 Da [This is the MW of the unprocessed precursor] CRC64: 48FDEEBAB8546737 [This is a checksum on the sequence] 
        10         20         30         40         50         60 
MGETIASEEM HRYFDGLEAR LKEAIEIANR ARARGGDPRP VVEIPLAKDL ADRVENLIGV 

        70         80         90        100        110        120 
KGVAEKIREL EARMSREEAA LEIGKQVAEG VVGSFPSKKD AVEAAIRVSM AVLTEGVVAA 

       130        140        150        160        170        180 
PIEGIDKVDL GKNDDGSQYI RIFYSGPIRS AGGTAQALSV LVGDYVRRGI GIDRYKPREE 

       190        200        210        220        230        240 
EVERYVEEIL LYKRVASLQY TPSEDEIRLI VRNCPVCIDG DPTEEAEVEG HRDLERIGTN 

       250        260        270        280        290        300 
RVRGGMCLVL AEGLALKAPK VKKHVNKLKM DGWDWLETLI GGAKSGESDE DEQKNKIKPK 

       310        320        330        340        350        360 
DKYIRDLIAG RPVFSHPSRP GGFRLRYGRS RNTSFASAGI NPAGMVLLDD FITNGTQLKI 

       370        380        390        400        410        420 
ERPGKAAAMS AVDSIEGPTV RLFSGDLIRV DDIKEAYEVR QQVEVIVDIG EILINYGDFL 

       430        440        450        460        470        480 
ENNHPLMPSP YVFEWWIYDY ESVCSEKILE KDLKNPSASL ALKLAEKYNV PLHPKFTYLW 

       490        500        510        520        530        540 
HDINRNEFEA LRKFVAEKGT FLEGEGEGEG EGILKLPLED SVKEGIKPVL EKLLVLHKVK 

       550        560        570        580        590        600 
EGGIFVEEAL PFILCLGLDR SLKEKVSMPD TGDMVEAAGI LSGFKVYPKA PSRIGARMGR 

       610        620        630        640        650        660 
PEKSDLRKMS PAAQVLFPIS NAGGMTRNLV SASDYTSCMN AKIGEIEVEL GLRECPACGK 

       670        680        690        700        710        720 
ESYFWRCECG EFTNPKLSCP RCKIDVRGAE TCPKCGRKPT SVANVKLDFR PIYKQAFENV 

       730        740        750        760        770        780 
GERERMDIIK GVKRLMNGQM TPEPLEKGIL RAKHDVYIFK DGTVRYDMSD IPLTHIRADE 

       790        800        810        820        830        840 
LGITAARLRE LDYKEDIYGK PLERDDQVVC LKVQDLLLSY DGAEYMLRTA KYVDELLVKY 

       850        860        870        880        890        900 
YKVEPYYNAE TIQDLVGVLM IGLAPHTSAG VLGRLIGFTK ASVGYAHPFF HASKRRNCDG 

       910        920        930        940        950        960 
DEDCVMLLMD GILNFSRSYL PEKRGGKMDA PLVLTTRIDP KEVDKEAHNI DLLARYPLEF 

       970        980        990       1000       1010       1020 
YRATQEIKNP TEIESIMDLV SSRLGKPDQY EHFMFTHDTS NIAAGPLKSS YKTLGSMIEK 

      1030       1040       1050       1060       1070       1080 
MEAQLSLAGK IRAVDAPDVA ERVLKSHFLP DLIGNLRSFS RQRMRCIKCG EKFRRPPLTG 

      1090       1100       1110       1120       1130       1140 
ACPKCGGNVV LTVHEGAVRK YLEISKEIGE RYGVSSYTRQ RIELLDYDIC SLFENHKVKQ 

      1150 
LGLSDFMSGS AR
Q8PXH2 in FASTA format

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