NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).

Comments

CATALYTIC ACTIVITY: (S)-dihydroorotate + O₂ = orotate + H₂O₂.
COFACTOR: Binds 1 FMN per subunit (By similarity).
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6 .
SUBUNIT: Homodimer (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein (By similarity).
SIMILARITY: Belongs to the dihydroorotate dehydrogenase family. Type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000040; BAC46405.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_767780.1; -.

3D structure databases

HSSP

Q02127; 1D3G. [HSSP ENTRY / PDB]

ModBase

Q89VA7.

Enzyme and pathway databases

BioCyc

BJAP224911:BLR1140-MON; -.

Ontologies

GO:0005886;	Cellular component: plasma membrane (inferred from electronic annotation from HAMAP).
GO:0004158;	Molecular function: dihydroorotate oxidase activity (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00225; -; 1.
PBIL [Tree]

InterPro

IPR013785; Aldolase_TIM.
IPR012135; DHO_DHase_1_2.
IPR005719; DHO_DHase_2.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000164; DHO_oxidase; 1.

TIGRFAMs

TIGR01036; pyrD_sub2; 1.

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.

BLOCKS

Q89VA7.

Genome annotation databases

GeneID

1049752; -.

GenomeReviews

BA000040_GR; blr1140.

KEGG

bja:blr1140; -.

NMPDR

fig|224911.1.peg.1140; -.

Phylogenomic databases

HOGENOM

Q89VA7; -.

Genome annotation databases

CMR

Q89VA7; blr1140.

Other

ProtoNet

Q89VA7.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cell membrane; Complete proteome; Flavoprotein; FMN; Membrane; Oxidoreductase; Pyrimidine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 365`	`365`	`Dihydroorotate dehydrogenase.`	`PRO_1000024155`
`ACT_SITE`	`173 173`		`Nucleophile (By similarity).`

Sequence information

Length: 365 AA [This is the length of the unprocessed precursor]

Molecular weight: 39336 Da [This is the MW of the unprocessed precursor]

CRC64: 98FEBC2B6DA8C744 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIRAFDALSL PVLRWLDPED AHRLAIQGLR FLPPVKPRAD DPKLAVRAFG LNFPNPIGMA 

        70         80         90        100        110        120 
AGFDKSAEVP DALLRLGFGF VEIGSVTPKP QSGNPRPRLF RLERDEAVIN RMGFNNDGAD 

       130        140        150        160        170        180 
VALRRLAARA QHGGIVGVNV GANKDSPDRV GDYVKLIEAF APVASYFTIN VSSPNTPGLR 

       190        200        210        220        230        240 
NLQEGALLDD LLARVIDARE RVRQKAGDTP VLLKIAPDLS LAQLDDVVQV ARSRRVDGMI 

       250        260        270        280        290        300 
VSNTTIARPS TLREEMRAKE QGGLSGRPLF RLSTRMVAET YVRVEGAFPL VGVGGVDSGG 

       310        320        330        340        350        360 
AALTKIRAGA SLIQLYSSLV YKGLGLVEEI KRDLTSTLLR TGRDSLSDIV GADAATLTAE 


DWPGM

Q89VA7 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools