NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=USDA 110;
DOI=10.1093/dnares/9.6.189; PubMed=12597275 [NCBI, ExPASy, EBI, Israel, Japan]
Kaneko T., Nakamura Y., Sato S., Minamisawa K., Uchiumi T., Sasamoto S., Watanabe A., Idesawa K., Iriguchi M., Kawashima K., Kohara M., Matsumoto M., Shimpo S., Tsuruoka H., Wada T., Yamada M., Tabata S.;
"Complete genomic sequence of nitrogen-fixing symbiotic bacterium Bradyrhizobium japonicum USDA110.";
DNA Res. 9:189-197(2002).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BA000040; BAC50061.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_771436.1; -.

3D structure databases

ModBase

Q89KV4.

Enzyme and pathway databases

BioCyc

BJAP224911:BLR4796-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

Q89KV4.

Genome annotation databases

GeneID

1052225; -.

GenomeReviews

BA000040_GR; blr4796.

KEGG

bja:blr4796; -.

NMPDR

fig|224911.1.peg.4796; -.

Phylogenomic databases

HOGENOM

Q89KV4; -.

Genome annotation databases

CMR

Q89KV4; blr4796.

Other

ProtoNet

Q89KV4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 153`	`153`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000162963`

Sequence information

Length: 153 AA [This is the length of the unprocessed precursor]

Molecular weight: 17352 Da [This is the MW of the unprocessed precursor]

CRC64: 09DB6F805C99F335 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTTMAKKSLQ LGRAVEWPHT PEEAQLDRVP NPQKGTDYLV RFTVPEFTSL CPVTGQPDFA 

        70         80         90        100        110        120 
HLMIDYAPGP WLLESKSLKL YIASFRNHGA FHEDCTVMIG KRIASEIKPK WLRIGGYWYP 

       130        140        150 
RGGIPIDVFW QTGRVPKGLW VPEQGVAPYR GRG

Q89KV4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools