NUCLEOTIDE SEQUENCE [GENOMIC DNA].
STRAIN=ATCC 58438 / CBS 3082 / IFO 1685 / JCM 7257 / NRRL Y-12651;
Hall C.R., Dietrich F.S.;
"Saccharomyces kluyveri gene encoding the biosynthetic enzyme dihydroorotate dehydrogenase family 1a.";
Submitted (JUN-2003) to the EMBL/GenBank/DDBJ databases.

[2]

NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND CHARACTERIZATION.
DOI=10.1007/s00438-004-0995-7; PubMed=15014982 [NCBI, ExPASy, EBI, Israel, Japan]
Gojkovic Z., Knecht W., Zameitat E., Warneboldt J., Coutelis J.B., Pynyaha Y., Neuveglise C., Moller K., Loffler M., Piskur J.;
"Horizontal gene transfer promoted evolution of the ability to propagate under anaerobic conditions in yeasts.";
Mol. Genet. Genomics 271:387-393(2004).

Comments

CATALYTIC ACTIVITY: (S)-dihydroorotate + O₂ = orotate + H₂O₂.
COFACTOR: Binds 1 FAD per subunit (By similarity).
ENZYME REGULATION: The activity is independent of the presence of oxygen.
PATHWAY: Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from HCO(3)(-): step 4/6 .
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
MISCELLANEOUS: S.kluyveri has two isoforms of DHODase, a cytoplasmic isoform and a mitochondrial isoform.
SIMILARITY: Belongs to the dihydroorotate dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY323902; AAQ01779.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF452109; AAQ04683.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

SMR

Q7Z892; 5-314.

ModBase

Q7Z892.

Family and domain databases

InterPro

IPR013785; Aldolase_TIM.
IPR012135; DHO_DHase_1_2.
IPR005720; DHO_DHase_1_core.
IPR001295; Dihydroorotate_DHase_core.
Graphical view of domain structure.

Gene3D

G3DSA:3.20.20.70; Aldolase_TIM; 1.

Pfam

PF01180; DHO_dh; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF000164; DHO_oxidase; 1.

TIGRFAMs

TIGR01037; pyrD_sub1_fam; 1.

PROSITE

PS00911; DHODEHASE_1; 1.
PS00912; DHODEHASE_2; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Pyrimidine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 314`	`314`	`Dihydroorotate dehydrogenase.`	`PRO_0000148503`
`ACT_SITE`	`133 133`		`Nucleophile (By similarity).`

Sequence information

Length: 314 AA [This is the length of the unprocessed precursor]

Molecular weight: 34855 Da [This is the MW of the unprocessed precursor]

CRC64: C7501535D7800D84 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSASLAINFL NHTYENPFMN ASGVHCMSTK ELDELKDSRA GAFITKSSTT SKREGNPEPR 

        70         80         90        100        110        120 
YFSVPLGSIN SMGLPNEGFD YYLKYALEYQ KNGSTSTPLF FSVAGMSVEE NLKMLQKIQD 

       130        140        150        160        170        180 
SDFNGITELN LSCPNVPGKP QVAYDFELTK EILTKVFEFF KKPLGVKLPP YFDFAHFDIM 

       190        200        210        220        230        240 
AGILNQLPLS YVNCINSIGN GLYINVETES VVVKPKNGFG GIGGEYVKPT ALANVRAFYT 

       250        260        270        280        290        300 
RLNPTIKIIG TGGIKTGQDA FEHLLCGATM LQVGTELYKE GVSIFDRLER ELKELMDKKG 

       310 
YTSIEQFRGK LNSL

Q7Z892 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools