NUCLEOTIDE SEQUENCE [MRNA], AND FUNCTION.
STRAIN=ATCC 56838;
DOI=10.1099/mic.0.26311-0; PubMed=12904551 [NCBI, ExPASy, EBI, Israel, Japan]
Namekawa S., Ichijima Y., Hamada F., Kasai N., Iwabata K., Nara T., Teraoka H., Sugawara F., Sakaguchi K.;
"DNA ligase IV from a basidiomycete, Coprinus cinereus, and its expression during meiosis.";
Microbiology 149:2119-2128(2003).

Comments

FUNCTION: Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining.
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
SIMILARITY: Contains 2 BRCT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AB098474; BAC76766.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AB072455; BAD93669.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q7Z7W5.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0003677;	Molecular function: DNA binding (inferred from electronic annotation from InterPro).
GO:0003910;	Molecular function: DNA ligase (ATP) activity (inferred from electronic annotation from InterPro).
GO:0006310;	Biological process: DNA recombination (inferred from electronic annotation from InterPro).
GO:0006281;	Biological process: DNA repair (inferred from electronic annotation from InterPro).
GO:0006260;	Biological process: DNA replication (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001357; BRCT.
IPR000977; DNA_ligase.
IPR012309; DNA_ligase_A_C.
IPR012310; DNA_ligase_A_M.
IPR012308; DNA_ligase_A_N.
IPR016059; DNA_ligase_CS.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF00533; BRCT; 2.
PF04679; DNA_ligase_A_C; 1.
PF01068; DNA_ligase_A_M; 1.
PF04675; DNA_ligase_A_N; 1.
Pfam graphical view of domain structure.

SMART

SM00292; BRCT; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS50172; BRCT; 2.
PS00697; DNA_LIGASE_A1; 1.
PS00333; DNA_LIGASE_A2; 1.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q7Z7W5.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1025`	`1025`	`DNA ligase 4.`	`PRO_0000278380`
`DOMAIN`	`667 763`	`97`	`BRCT 1.`
`DOMAIN`	`915 1025`	`111`	`BRCT 2.`
`ACT_SITE`	`291 291`		`N6-AMP-lysine intermediate (By similarity).`

Sequence information

Length: 1025 AA [This is the length of the unprocessed precursor]

Molecular weight: 116825 Da [This is the MW of the unprocessed precursor]

CRC64: ACAE9D076932868C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MMQPTPAPSS APGSPQRTQA EPEMETPSYP QPPQNVGTAP FSVLVKLFEK LATERKQERR 

        70         80         90        100        110        120 
RKLLDAWFRH WRREKGFDLY PVLRLLLPQK DRDRAVYGLK EKNLAKTYIK LIPLGMRDPD 

       130        140        150        160        170        180 
AIRLLNWKKP TERDKSSGDF PQVLCEVVSK RSSVIEGTLT IDELNEILDD IAKNMGKSDV 

       190        200        210        220        230        240 
QSKILRRIYN NSTADEQRWI IRIILKDMNI SVKETTVFAV FHPDAQDLYN TCSDLKKVAW 

       250        260        270        280        290        300 
ELWDPSRRLN AKDKEIQIFH AFAPMLCKRP TRKIEETVKA MGGSKFIIEE KLDGERMQLH 

       310        320        330        340        350        360 
KRGNEYFYCS RKGKDYTYLY GKHIGAGSLT PFIDSAFDSR IDDIILDGEM LVWDPVSERN 

       370        380        390        400        410        420 
LPFGTLKTAA LDRSKKENNP RPCFKVFDLL YLNGMSLLDK TVKFRKNNLR HCIKPIPGRI 

       430        440        450        460        470        480 
EFVEEYQGET ANDIRKRMEQ VMENRGEGLV IKHPKAKYIL NGRNTDWIKV KPEYMDNMGE 

       490        500        510        520        530        540 
TVDVLVVAGN YGSGKRGGGV STLICAVMDD RRPDSDDEPK YSSFVRIGTG LSFADYVWVR 

       550        560        570        580        590        600 
SKPWKVWDPK NPPEFLQTAK KGQEDKGDVY LEPEDSFILK VKAAEITPSD QYHMGFTMRF 

       610        620        630        640        650        660 
PRALAIRDDL SIADCMTATE VFESLKSERK RKMEDDAGIT TKKRKTTVKK VALLPEYSGP 

       670        680        690        700        710        720 
NLKKVAVKTD IFNGMKFVVF SDPKSRTGEA DKKELMKTIH ANGGTCSQIV NKNSEAIVIY 

       730        740        750        760        770        780 
GGSITPYDLK LVIDKGIHDV IKPSWITDSV TLGEPAPFKK KYFFHATEER KYADEYNEDD 

       790        800        810        820        830        840 
GEEEGAVPSA DEQERDVKSG TVEPGSETED EDEEQAPEIK EEQDGELHEW LKVDDRKSPA 

       850        860        870        880        890        900 
LPAHDEEDSV TEDDSDNADV ADEEEPDLDD WFQVKGETED EGAGALATAS RHRETTPDVD 

       910        920        930        940        950        960 
GDVKMGESEE AMDYDPDVIF KHLCFYLDSP ANAQRHGMAT RPKYEAAITK SFEEVEKLIK 

       970        980        990       1000       1010       1020 
DNGGKIVDLD EPKLTHVVLD KRDDSRRVEL MKRTSKPRRR HLVLSDYIEA CIDEGTLLDE 


EEFAP

Q7Z7W5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools