ID   QUEF_BORBR              Reviewed;         273 AA.
AC   Q7WH69;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   22-NOV-2005, sequence version 2.
DT   25-NOV-2008, entry version 37.
DE   RecName: Full=NADPH-dependent 7-cyano-7-deazaguanine reductase;
DE            EC=1.7.1.13;
DE   AltName: Full=7-cyano-7-carbaguanine reductase;
DE   AltName: Full=PreQ(0) reductase;
DE   AltName: Full=NADPH-dependent nitrile oxidoreductase;
GN   Name=queF; OrderedLocusNames=BB3340;
OS   Bordetella bronchiseptica (Alcaligenes bronchisepticus).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Alcaligenaceae; Bordetella.
OX   NCBI_TaxID=518;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RB50 / ATCC BAA-588 / NCTC 13252;
RX   MEDLINE=22827954; PubMed=12910271; DOI=10.1038/ng1227;
RA   Parkhill J., Sebaihia M., Preston A., Murphy L.D., Thomson N.R.,
RA   Harris D.E., Holden M.T.G., Churcher C.M., Bentley S.D., Mungall K.L.,
RA   Cerdeno-Tarraga A.-M., Temple L., James K.D., Harris B., Quail M.A.,
RA   Achtman M., Atkin R., Baker S., Basham D., Bason N., Cherevach I.,
RA   Chillingworth T., Collins M., Cronin A., Davis P., Doggett J.,
RA   Feltwell T., Goble A., Hamlin N., Hauser H., Holroyd S., Jagels K.,
RA   Leather S., Moule S., Norberczak H., O'Neil S., Ormond D., Price C.,
RA   Rabbinowitsch E., Rutter S., Sanders M., Saunders D., Seeger K.,
RA   Sharp S., Simmonds M., Skelton J., Squares R., Squares S., Stevens K.,
RA   Unwin L., Whitehead S., Barrell B.G., Maskell D.J.;
RT   "Comparative analysis of the genome sequences of Bordetella pertussis,
RT   Bordetella parapertussis and Bordetella bronchiseptica.";
RL   Nat. Genet. 35:32-40(2003).
CC   -!- FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-
CC       deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By
CC       similarity).
CC   -!- CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP(+) = 7-
CC       cyano-7-carbaguanine + 2 NADPH.
CC   -!- PATHWAY: tRNA modification; queuosine-tRNA biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (Probable).
CC   -!- SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type
CC       2 subfamily.
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DR   EMBL; BX640447; CAE33832.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; NP_889875.2; -.
DR   GeneID; 2660959; -.
DR   GenomeReviews; BX470250_GR; BB3340.
DR   KEGG; bbr:BB3340; -.
DR   NMPDR; fig|257310.1.peg.3322; -.
DR   HOGENOM; Q7WH69; -.
DR   BioCyc; BBRO257310:BB3340-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0003934; F:GTP cyclohydrolase I activity; IEA:InterPro.
DR   GO; GO:0046857; F:oxidoreductase activity, acting on other ni...; IEA:HAMAP.
DR   GO; GO:0033739; F:queuine synthase activity; IEA:EC.
DR   GO; GO:0019438; P:aromatic compound biosynthetic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0008616; P:queuosine biosynthetic process; IEA:HAMAP.
DR   HAMAP; MF_00817; -; 1.
DR   InterPro; IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
DR   InterPro; IPR001474; GTP_CycOHase_I.
DR   Pfam; PF01227; GTP_cyclohydroI; 1.
DR   PIRSF; PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.
DR   TIGRFAMs; TIGR03138; QueF; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; NADP; Oxidoreductase;
KW   Queuosine biosynthesis.
FT   CHAIN         1    273       NADPH-dependent 7-cyano-7-deazaguanine
FT                                reductase.
FT                                /FTId=PRO_0000163020.
SQ   SEQUENCE   273 AA;  30651 MW;  C742FF9C344C50D2 CRC64;
     MSLSNAPLGQ HVAYPSQYDP GLLFPIPRAT NRASLQLGAT LPFTGVDLWN AYELSWLDAR
     GKPRVAMATF SFPADSPNIV ESKSFKLYLN SFNQTRLPNA QALRDRLERD LAAAAGAPVG
     LEFISPQRFG ELNMAELDGI YIDKLDIEID TYEPAPQLLQ CAPGDEVEET LATRLLKSNC
     PVTGQPDWAS LQVRYRGRPI DRAALLKYVV SFRQHAEFHE HCVERIFGDI MRACQPRQLT
     VYARYTRRGG LDINPWRSNF ESAPPADVRT ARQ
//