CATALYTIC ACTIVITY: 2 NO + 2 O₂ + NAD(P)H = 2 NO₃^- + NAD(P)⁺.
COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group per subunit (By similarity).
COFACTOR: Binds 1 FAD per subunit (By similarity).
DOMAIN: Consists of two distinct domains; an N-terminal heme-containing oxygen-binding domain and a C-terminal reductase domain with binding sites for FAD and NAD(P)H.
SIMILARITY: Belongs to the globin family. Two-domain flavohemoproteins subfamily.
SIMILARITY: In the C-terminal section; belongs to the flavoprotein pyridine nucleotide cytochrome reductase family.
SIMILARITY: Contains 1 FAD-binding FR-type domain.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BX294154; CAD77481.1; ALT_INIT; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_870404.1; -.

3D structure databases

ModBase

Q7UIY1.

Enzyme and pathway databases

BioCyc

PSP117:RB12262-MON; -.

Ontologies

GO:0008941;	Molecular function: nitric oxide dioxygenase activity (inferred from electronic annotation from HAMAP).
GO:0005344;	Molecular function: oxygen transporter activity (inferred from electronic annotation from HAMAP).
GO:0015671;	Biological process: oxygen transport (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_01252; -; 1.
PBIL [Tree]

InterPro

IPR001834; Cyt_B5_reductase.
IPR012292; Globin.
IPR000971; Globin_subset.
IPR008333; OxRdtase_FAD-bd.
IPR001433; OxRdtase_FAD/NAD_bd.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.490.10; Globin_related; 1.

Pfam

PF00970; FAD_binding_6; 1.
PF00042; Globin; 1.
PF00175; NAD_binding_1; 1.
Pfam graphical view of domain structure.

PRINTS

PR00406; CYTB5RDTASE.

PROSITE

PS51384; FAD_FR; 1.
PS01033; GLOBIN; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q7UIY1.

Genome annotation databases

GeneID

1794152; -.

GenomeReviews

BX119912_GR; RB12262.

KEGG

rba:RB12262; -.

NMPDR

fig|243090.1.peg.6744; -.

Phylogenomic databases

HOGENOM

Q7UIY1; -.

Genome annotation databases

CMR

Q7UIY1; RB12262.

Other

ProtoNet

Q7UIY1.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; FAD; Flavoprotein; Heme; Iron; Metal-binding; NAD; NADP; Oxidoreductase; Oxygen transport; Transport.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 408`	`408`	`Flavohemoprotein.`	`PRO_0000052443`
`DOMAIN`	`152 263`	`112`	`FAD-binding FR-type.`
`NP_BIND`	`205 208`	`4`	`FAD (By similarity).`
`NP_BIND`	`277 282`	`6`	`NADP (By similarity).`
`NP_BIND`	`398 401`	`4`	`FAD (By similarity).`
`REGION`	`1 138`	`138`	`Globin.`
`REGION`	`149 408`	`260`	`Reductase.`
`REGION`	`267 408`	`142`	`NAD or NADP-binding.`
`ACT_SITE`	`95 95`		`Charge relay system (By similarity).`
`ACT_SITE`	`137 137`		`Charge relay system (By similarity).`
`METAL`	`85 85`		`Iron (heme proximal ligand) (By similarity).`
`BINDING`	`190 190`		`FAD (By similarity).`
`SITE`	`29 29`	`1`	`Involved in heme-bound ligand stabilization and O-O bond activation (By similarity).`
`SITE`	`84 84`	`1`	`Influences the redox potential of the prosthetic heme and FAD groups (By similarity).`
`SITE`	`397 397`	`1`	`Influences the redox potential of the prosthetic heme and FAD groups (By similarity).`

Sequence information

Length: 408 AA [This is the length of the unprocessed precursor]

Molecular weight: 44911 Da [This is the MW of the unprocessed precursor]

CRC64: B33B7EBC66316C1C [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLSEKTIRIV KEITPLVAAN AETITRRFYE RMFEANPEVK AFFNQAHQHS GGQQKALAGA 

        70         80         90        100        110        120 
ICAYFTHIDN PAVLMPAVEL IAQKHVSLGI KPEHYPIVGS NLLAAIGDVM GDAATPEIVE 

       130        140        150        160        170        180 
AVSEAYGFLA DIFIGREGAI YEEQASMPGG WNGTRTFVVT KKVRESEIVT SFYLKPEDEG 

       190        200        210        220        230        240 
PLPPFKPGQY ITVHMDHPHT PTSPRNYSLS DCASQPHYRI SVKREERLVP DAPDGLISNH 

       250        260        270        280        290        300 
LHDGIEEGHR IELGPPCGEF TVDPATIAKP IVLIAGGIGV TPLLSMAKSI VHANPNAELH 

       310        320        330        340        350        360 
FIQAARNSKV HAFADELRRL AQAGPNVHTK VIYDSPLPGD VEEGKCDEAG FVTENQIRES 

       370        380        390        400 
TPFTDADFYF CGPKPFMKNV HSCLRELGVD EHRVRYEFFG PKEELVAV

Q7UIY1 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools