ID   MAO12_VIBVY             Reviewed;         559 AA.
AC   Q7MJC0;
DT   06-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   15-DEC-2003, sequence version 1.
DT   04-NOV-2008, entry version 34.
DE   RecName: Full=NAD-dependent malic enzyme 2;
DE            Short=NAD-ME 2;
DE            EC=1.1.1.38;
GN   Name=sfcA2; OrderedLocusNames=VV2242;
OS   Vibrio vulnificus (strain YJ016).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales;
OC   Vibrionaceae; Vibrio.
OX   NCBI_TaxID=196600;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14656965; DOI=10.1101/gr.1295503;
RA   Chen C.-Y., Wu K.-M., Chang Y.-C., Chang C.-H., Tsai H.-C.,
RA   Liao T.-L., Liu Y.-M., Chen H.-J., Shen A.B.-T., Li J.-C., Su T.-L.,
RA   Shao C.-P., Lee C.-T., Hor L.-I., Tsai S.-F.;
RT   "Comparative genome analysis of Vibrio vulnificus, a marine
RT   pathogen.";
RL   Genome Res. 13:2577-2587(2003).
CC   -!- CATALYTIC ACTIVITY: (S)-malate + NAD(+) = pyruvate + CO(2) + NADH.
CC   -!- COFACTOR: Divalent metal cations. Prefers magnesium or manganese
CC       (By similarity).
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SIMILARITY: Belongs to the malic enzymes family.
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DR   EMBL; BA000037; BAC95006.1; -; Genomic_DNA.
DR   RefSeq; NP_935035.1; -.
DR   HSSP; P27443; 1O0S.
DR   GeneID; 2625048; -.
DR   GenomeReviews; BA000037_GR; VV2242.
DR   KEGG; vvy:VV2242; -.
DR   NMPDR; fig|196600.1.peg.2310; -.
DR   HOGENOM; Q7MJC0; -.
DR   BioCyc; VVUL196600:VV2242-MON; -.
DR   GO; GO:0016619; F:malate dehydrogenase (oxaloacetate-decarbox...; IEA:HAMAP.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_01619; -; 1.
DR   InterPro; IPR015884; Malic_enzyme_CS.
DR   InterPro; IPR012301; Malic_N.
DR   InterPro; IPR012302; Malic_NAD_bd.
DR   InterPro; IPR001891; Malic_OxRdtase.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF00390; malic; 1.
DR   Pfam; PF03949; Malic_M; 1.
DR   PRINTS; PR00072; MALOXRDTASE.
DR   PROSITE; PS00331; MALIC_ENZYMES; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Metal-binding; NAD; Oxidoreductase.
FT   CHAIN         1    559       NAD-dependent malic enzyme 2.
FT                                /FTId=PRO_0000160239.
FT   ACT_SITE     98     98       Proton donor (By similarity).
FT   ACT_SITE    169    169       Proton acceptor (By similarity).
FT   METAL       240    240       Divalent metal cation (By similarity).
FT   METAL       241    241       Divalent metal cation (By similarity).
FT   METAL       264    264       Divalent metal cation (By similarity).
FT   BINDING     151    151       NAD (By similarity).
FT   BINDING     264    264       NAD (By similarity).
FT   BINDING     413    413       NAD (By similarity).
FT   SITE        264    264       Important for activity (By similarity).
SQ   SEQUENCE   559 AA;  62073 MW;  402FC1DAC6689471 CRC64;
     MSKKMFIRQA GNTLLNTPLL NKGSAFTLEE RKNFNLIGLL PANIETIDEQ VSRAYEQFSL
     FNSAMEKHIY LRNIQDTNET LYFRLINQHI EEMMPIIYTP TVGEACQKFS QIYRRNRGLF
     LSFEDQDELE AILNNAPNTH VKVIVITDGE RILGLGDQGI GGMGIPIGKL ALYTACGGIS
     PEHTLPIVLD VGTNNSALLS DPMYMGWRHP RITGDQYDDF VDDCLKAIRR RWPNALIQFE
     DFAQANAMPL LMRYQNQFCC FNDDIQGTAS VTVGTLLAAA HATGKKLSAQ KVLFAGAGSA
     GCGIAEAIVA QMVSEGISVQ QARSQVFMVD RWGMLEQEMP NLLPFQKPLA QPASLRTEWQ
     IEANREISLL DVIQHAHPDV LIGVTGVPGL FNQEIIEAMA EDCERPVVMP LSNPTSRVEA
     KPEDILMWTQ GQAIVATGSP FPDVVLAGKR YPIAQCNNSY IFPGVGLGVI SANAHRVTNE
     MLQQASITLA SLSPMLNGGN MLLPPLSEIQ NVSRKIALEV AKKAVEQGKA SHRTEERLLE
     RIDEEFWYAQ YCEYRRIAS
//