NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 10895 / NRRL Y-1056 / CBS 109.51;
DOI=10.1126/science.1095781; PubMed=15001715 [NCBI, ExPASy, EBI, Israel, Japan]
Dietrich F.S., Voegeli S., Brachat S., Lerch A., Gates K., Steiner S., Mohr C., Poehlmann R., Luedi P., Choi S., Wing R.A., Flavier A., Gaffney T.D., Philippsen P.;
"The Ashbya gossypii genome as a tool for mapping the ancient Saccharomyces cerevisiae genome.";
Science 304:304-307(2004).

Comments

FUNCTION: Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code (By similarity).
CATALYTIC ACTIVITY: Protein N₆,N⁶-dimethyl-L-lysine + 2-oxoglutarate + O₂ = protein N⁶-methyl-L-lysine + succinate + formaldehyde + CO₂.
CATALYTIC ACTIVITY: Protein N⁶-methyl-L-lysine + 2-oxoglutarate + O₂ = protein L-lysine + succinate + formaldehyde + CO₂.
COFACTOR: Binds 1 Fe(2+) ion per subunit (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).
DOMAIN: The JmjC domain mediates the demethylation activity (By similarity).
SIMILARITY: Belongs to the JHDM1 histone demethylase family.
SIMILARITY: Contains 1 JmjC domain.
SIMILARITY: Contains 1 PHD-type zinc finger.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AE016817; AAS51832.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_984008.1; -.

3D structure databases

ModBase

Q75AL5.

Organism-specific databases

AGD

ADL088W; -.

Family and domain databases

InterPro

IPR003347; TF_JmjC_AAH.
IPR001965; Znf_PHD.
Graphical view of domain structure.

SMART

SM00558; JmjC; 1.
SM00249; PHD; 1.
SMART graphical view of domain structure.

PROSITE

PS51184; JMJC; 1.
PS01359; ZF_PHD_1; FALSE_NEG.
PS50016; ZF_PHD_2; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q75AL5.

Genome annotation databases

4620150; -.

fig|33169.1.peg.1653; -.

Phylogenomic databases

HOGENOM

Q75AL5; -.

Other

ProtoNet

Q75AL5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chromatin regulator; Complete proteome; Dioxygenase; Iron; Metal-binding; Nucleus; Oxidoreductase; Transcription; Transcription regulation; Zinc; Zinc-finger.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 486`	`486`	`JmjC domain-containing histone demethylation protein 1.`	`PRO_0000226791`
`DOMAIN`	`245 404`	`160`	`JmjC.`
`ZN_FING`	`28 83`	`56`	`PHD-type; degenerate.`
`METAL`	`300 300`		`Iron; catalytic (By similarity).`
`METAL`	`302 302`		`Iron; catalytic (By similarity).`
`METAL`	`372 372`		`Iron; catalytic (By similarity).`
`BINDING`	`297 297`		`Substrate (By similarity).`
`BINDING`	`317 317`		`Substrate (By similarity).`

Sequence information

Length: 486 AA [This is the length of the unprocessed precursor]

Molecular weight: 55076 Da [This is the MW of the unprocessed precursor]

CRC64: 53A9F8861DCACDC7 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLQQVTSTTV TGADIQLRAK LLLFSMTDDQ CVYCKGKMEH RMWVQCEACP QWVHVQCIPE 

        70         80         90        100        110        120 
ECLSGGEYPS RSSDIAAFEC SAHGTARARL ALKGKRRRVE AKEEPERAGT RRYRLRKRGP 

       130        140        150        160        170        180 
LDYIALNEGQ DVRLRHEHPH RAAFQGCFTK WSGLGRTVTS AELQQSFAEL REPVLVADPE 

       190        200        210        220        230        240 
HSGMQTPAMD EQVLADVLGA DYSLDVMDVQ SQQNERWTMG QWKEYMHTAR GVRDRIRNVI 

       250        260        270        280        290        300 
SLEVSHVPEF GQRIRRPRAV EDNDLVDLVW PVQPAPEIGA KPKVQKYVLM SAANAYTDFH 

       310        320        330        340        350        360 
LDFAGTSVYY SLLRGAKQFL LFPPTPANLG AYKAWCADDN QGLIFLGDRL QDGVLFSLRP 

       370        380        390        400        410        420 
GDLFMIPSGF IHAVYTPEDS FVVGGNYLCL RDLSTHIRIV RIEQETQVPK KFTFPKFERV 

       430        440        450        460        470        480 
MGLTAEWLLE GLPERLQLIT HEHAVALLDY LRDTRLKYKP AHYHTKSTML ASLEKALEGC 


EPASGP

Q75AL5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools