ID   KO1_GIBIN               Reviewed;         525 AA.
AC   Q701P2;
DT   20-DEC-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-JUL-2004, sequence version 1.
DT   25-NOV-2008, entry version 33.
DE   RecName: Full=Ent-kaurene oxidase;
DE            EC=1.14.13.78;
DE   AltName: Full=Cytochrome P450-4;
DE   AltName: Full=Cytochrome P450 503A1;
GN   Name=CYP503A1;
OS   Gibberella intermedia (Bulb rot disease fungus) (Fusarium
OS   proliferatum).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina;
OC   Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae;
OC   Gibberella; Gibberella fujikuroi complex.
OX   NCBI_TaxID=42674;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=D02945;
RX   PubMed=15746349; DOI=10.1128/AEM.71.3.1462-1472.2005;
RA   Malonek S., Rojas M.C., Hedden P., Gaskin P., Hopkins P.,
RA   Tudzynski B.;
RT   "Functional characterization of two cytochrome P450 monooxygenase
RT   genes, P450-1 and P450-4, of the gibberellic acid gene cluster in
RT   Fusarium proliferatum (Gibberella fujikuroi MP-D).";
RL   Appl. Environ. Microbiol. 71:1462-1472(2005).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=16204516; DOI=10.1128/AEM.71.10.6014-6025.2005;
RA   Malonek S., Rojas M.C., Hedden P., Hopkins P., Tudzynski B.;
RT   "Restoration of gibberellin production in Fusarium proliferatum by
RT   functional complementation of enzymatic blocks.";
RL   Appl. Environ. Microbiol. 71:6014-6025(2005).
CC   -!- FUNCTION: Catalyzes three successive oxidations of the 4-methyl
CC       group of ent-kaurene giving kaurenoic acid, a key step in
CC       gibberellin (GA) biosynthesis.
CC   -!- CATALYTIC ACTIVITY: Ent-kaur-16-ene + NADPH + O(2) = ent-kaur-16-
CC       en-19-ol + NADP(+) + H(2)O.
CC   -!- CATALYTIC ACTIVITY: Ent-kaur-16-en-19-ol + NADPH + O(2) = ent-
CC       kaur-16-en-19-al + NADP(+) + 2 H(2)O.
CC   -!- CATALYTIC ACTIVITY: Ent-kaur-16-en-19-al + NADPH + O(2) = ent-
CC       kaur-16-en-19-oate + NADP(+) + H(2)O.
CC   -!- COFACTOR: Heme group (By similarity).
CC   -!- PATHWAY: Plant hormone biosynthesis; gibberellin biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Membrane; Single-pass membrane protein
CC       (Potential).
CC   -!- MISCELLANEOUS: This protein is poorly expressed and has no
CC       detectable ent-kaurene oxidase activity in this species.
CC       Consequently, this species does not produce gibberellins.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family.
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DR   EMBL; AJ628021; CAF31352.1; -; Genomic_DNA.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0009055; F:electron carrier activity; IEA:InterPro.
DR   GO; GO:0010241; F:ent-kaurene oxidase activity; IEA:EC.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   Gene3D; G3DSA:1.10.630.10; Cyt_P450; 1.
DR   PANTHER; PTHR19383; Cyt_P450; 1.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
PE   1: Evidence at protein level;
KW   Heme; Iron; Membrane; Metal-binding; Monooxygenase; NADP;
KW   Oxidoreductase; Transmembrane.
FT   CHAIN         1    525       Ent-kaurene oxidase.
FT                                /FTId=PRO_0000052209.
FT   TRANSMEM     31     51       Potential.
FT   METAL       466    466       Iron (heme axial ligand) (By similarity).
SQ   SEQUENCE   525 AA;  59974 MW;  4E05AE3CEC4D3451 CRC64;
     MNKSNSMNNT SLERLFQQLV LGLDGIPLMD VHWLIYVAFG AWLCSYVIHV LSSSSTVKVP
     VVGYRSVFEP TWLLRLRFVW EGGSIIGQGY NKFKDSIFQV RKLGTDIVII PPNFIDEVRK
     LSQDKTRSVE PFINDFAGQY TRGMVFLQSD LQNRVIQQRL TPKLVSLTKV MKEELDYALT
     KEIPDMKDDE WVEVDISSIM VRLISRISAR VFLGPEHCRN QEWLTNTAEY SESLFITGFI
     LRVVPHILRP FIAPLLPSYR TLLRNVSSGR RVIGDIIRSQ QGDGNEDILS WMRDAATGEE
     KQIDNIAQRM LILSLASIHT TAMTMTHAMY DLCARPEYIE PLRDEVKGVV DASGWDKTAL
     NRLHRLDSFL KESQRFNPVF LLTFNRIYHQ SMTLSDGTNL PSGTRIAVPS HAMLQDSAHV
     PGPTPPTEFD GFRYSKIRSD SNYAQKYLFS MTDSSNMAFG YGKYACPGRF YASNEMKLTL
     AILLLQFEFK LPDGKGRPRN ITIDSDMIPD PRARLCVRKR SLRDE
//