[1]	NUCLEOTIDE SEQUENCE [MRNA]. Hua X., Liu N., Liu H., Yang Y., Shan D., Zhang Y., Guo Y., Zhang M., Wu C., Li X., Zheng C.; "Cloning and characterization of GhZIP."; Submitted (NOV-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the formation of the isocyclic ring in chlorophyll biosynthesis. Mediates the cyclase reaction, which results in the formation of divinylprotochlorophyllide (Pchlide) characteristic of all chlorophylls from magnesium-protoporphyrin IX 13-monomethyl ester (MgPMME) (By similarity).
CATALYTIC ACTIVITY: Magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + H₂O.
CATALYTIC ACTIVITY: 13¹-hydroxy-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADP⁺ + 2 H₂O.
CATALYTIC ACTIVITY: 13¹-oxo-magnesium-protoporphyrin IX 13-monomethyl ester + NADPH + O₂ = divinylprotochlorophyllide + NADP⁺ + 2 H₂O.
COFACTOR: Iron (By similarity).
PATHWAY: Porphyrin metabolism; chlorophyll biosynthesis .
SUBCELLULAR LOCATION: Plastid, chloroplast (By similarity).
SIMILARITY: Belongs to the acsF family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY456957; AAR20445.2; ALT_INIT; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q6SJV8.

Family and domain databases

InterPro

IPR008434; AcsF.
IPR003251; Rubrerythrin.
Graphical view of domain structure.

Pfam

PF02915; Rubrerythrin; 1.
Pfam graphical view of domain structure.

TIGR02029; AcsF; 1.

Other

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Chlorophyll biosynthesis; Chloroplast; Iron; Metal-binding; NADP; Oxidoreductase; Photosynthesis; Plastid; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 43`	`43`	`Chloroplast (Potential).`
`CHAIN`	`44 405`	`362`	`Magnesium-protoporphyrin IX monomethyl ester [oxidative] cyclase, chloroplastic.`	`PRO_0000000602`

Sequence information

Length: 405 AA [This is the length of the unprocessed precursor]

Molecular weight: 47008 Da [This is the MW of the unprocessed precursor]

CRC64: 55BC9EEB09C0A807 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MATEMALVKP ISKFSTSSPI FSNSRYGKFT TVRMSSTSQS TTKAAAKGGK KAAKTAIKET 

        70         80         90        100        110        120 
LLTPRFYTTD FDEMEALFNT EINKNLNQSE FESLLQEFKT DYNQTHFVRN KEFKEAADKI 

       130        140        150        160        170        180 
DGPLRQIFVE FSERSCTAEF SGFLLYKELG RRLKKTNPVV AEIFSLMSRD EARHAGFLNK 

       190        200        210        220        230        240 
GLSDFNLALD LGFLTKARKY TFFKPKFIFY ATYLSEKIGY WRYITIYRHL KENPEYQCYP 

       250        260        270        280        290        300 
IFKYFENWCQ DENRHGDFFS ALLKAQPQFL NDWKAKLWSR FFCLSVYVTM YLNDCQRTAF 

       310        320        330        340        350        360 
YEGIGLDTKE FDMHVIIETN RTTARIFPAV LDVENPEFKR RLDKMVEINE QLLAVGETSD 

       370        380        390        400 
IPLVKNLKRI PLIAALASEL LATYLMPPIE SGSVDFAEFE PQLVY

Q6SJV8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools