[1]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). TISSUE=Liver; DOI=10.1074/jbc.M302149200; PubMed=12660232 [NCBI, ExPASy, EBI, Israel, Japan] Wu T., Yankovskaya V., McIntire W.S.; "Cloning, sequencing, and heterologous expression of the murine peroxisomal flavoprotein, N(1)-acetylated polyamine oxidase."; J. Biol. Chem. 278:20514-20525(2003).
[2]	NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 3; 4; 5; 6; 8; 9; 10; 11; 12 AND 13). Wang Y., Murray-Stewart T., Hacker A., Casero R.A. Jr.; "Polyamine oxidase."; Submitted (FEB-2004) to the EMBL/GenBank/DDBJ databases.
[3]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). DOI=10.1101/gr.1293003; PubMed=12975309 [NCBI, ExPASy, EBI, Israel, Japan] Clark H.F., Gurney A.L., Abaya E., Baker K., Baldwin D.T., Brush J., Chen J., Chow B., Chui C., Crowley C., Currell B., Deuel B., Dowd P., Eaton D., Foster J.S., Grimaldi C., Gu Q., Hass P.E., Heldens S., Huang A., Kim H.S., Klimowski L., Jin Y., Johnson S., Lee J., Lewis L., Liao D., Mark M.R., Robbie E., Sanchez C., Schoenfeld J., Seshagiri S., Simmons L., Singh J., Smith V., Stinson J., Vagts A., Vandlen R.L., Watanabe C., Wieand D., Woods K., Xie M.-H., Yansura D.G., Yi S., Yu G., Yuan J., Zhang M., Zhang Z., Goddard A.D., Wood W.I., Godowski P.J., Gray A.M.; "The secreted protein discovery initiative (SPDI), a large-scale effort to identify novel human secreted and transmembrane proteins: a bioinformatics assessment."; Genome Res. 13:2265-2270(2003).
[4]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). TISSUE=Brain; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[5]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 364-649 (ISOFORM 9). TISSUE=Testis; The German cDNA consortium; Submitted (JUL-2002) to the EMBL/GenBank/DDBJ databases.
[6]	BIOPHYSICOCHEMICAL PROPERTIES, CHARACTERIZATION, AND TISSUE SPECIFICITY. DOI=10.1042/BJ20021779; PubMed=12477380 [NCBI, ExPASy, EBI, Israel, Japan] Vujcic S., Liang P., Diegelman P., Kramer D.L., Porter C.W.; "Genomic identification and biochemical characterization of the mammalian polyamine oxidase involved in polyamine back-conversion."; Biochem. J. 370:19-28(2003).

Comments

FUNCTION: Flavoenzyme which catalyzes the oxidation of N(1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. Can also oxidize N(1)-acetylspermidine to putrescine. Substrate specificity: N(1)-acetylspermine = N(1)-acetylspermidine > N(1),N(12)-diacylspermine >> spermine. Does not oxidize spermidine. Plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.
CATALYTIC ACTIVITY: N¹-acetylspermine + O₂ + H₂O = N¹-acetylspermidine + 3-aminopropanal + H₂O₂.
COFACTOR: FAD (By similarity).
PATHWAY: Amine and polyamine metabolism; spermine metabolism .
SUBUNIT: Monomer (By similarity).
SUBCELLULAR LOCATION: Peroxisome (By similarity). Cytoplasm (By similarity).

ALTERNATIVE PRODUCTS: 11 named isoforms [FASTA] produced by alternative splicing.

Name	9
Isoform ID	Q6QHF9-1
This is the isoform sequence displayed in this entry.

Name	1
Isoform ID	Q6QHF9-2
Features which should be applied to build the isoform sequence: VSP_011249.

Name	3
Synonyms	7
Isoform ID	Q6QHF9-6
Features which should be applied to build the isoform sequence: VSP_011250, VSP_011251.

Name	4
Synonyms	2
Isoform ID	Q6QHF9-5
Features which should be applied to build the isoform sequence: VSP_011249, VSP_011255, VSP_011257.

Name	5
Isoform ID	Q6QHF9-4
Features which should be applied to build the isoform sequence: VSP_011249, VSP_011258.

Name	6
Isoform ID	Q6QHF9-8
Note: Ref.2 (AAS64377) sequence differs from that shown due to a frameshift in position 26.
Features which should be applied to build the isoform sequence: VSP_011248, VSP_011256.

Name	8
Isoform ID	Q6QHF9-3
Features which should be applied to build the isoform sequence: VSP_011249, VSP_011254.

Name	10
Isoform ID	Q6QHF9-7
Features which should be applied to build the isoform sequence: VSP_011252.

Name	11
Isoform ID	Q6QHF9-9
Features which should be applied to build the isoform sequence: VSP_011247, VSP_011253.

Name	12
Isoform ID	Q6QHF9-10
Features which should be applied to build the isoform sequence: VSP_011259, VSP_011260.

Name	13
Isoform ID	Q6QHF9-11
Features which should be applied to build the isoform sequence: VSP_011261, VSP_011262.

TISSUE SPECIFICITY: Widely expressed. Not detected in spleen. Expressed at lower level in neoplastic tissues.
INDUCTION: By polyamine analogs.
MISCELLANEOUS: Oxidizes N(1)-acetylated polyamines on the exo-side of their N(4)-amino groups. Plant PAO oxidizes spermine on the endo-side of the N(4)-nitrogen (By similarity).
SIMILARITY: Belongs to the flavin monoamine oxidase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AF226657; AAN40706.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF312698; AAO63265.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541513; AAS64373.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541514; AAS64374.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541515; AAS64375.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541516; AAS64376.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541517; AAS64377.1; ALT_FRAME; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541518; AAS64378.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541519; AAS64379.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541520; AAS64380.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541521; AAS64381.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541522; AAS64382.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541523; AAS64383.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY541524; AAS64384.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AY358418; AAQ88784.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC032778; AAH32778.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AL834535; CAD39191.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_690875.1; -.
NP_997010.1; -.
NP_997011.1; -.

UniGene

Hs.532469

3D structure databases

ModBase

Q6QHF9.

Enzyme and pathway databases

Reactome

REACT_2063; Metabolism of xenobiotics.

Organism-specific databases

H-InvDB

HIX0009334; -.
HIX0009335; -.

HGNC:20837; PAOX.

PA134907695; -.

Gene expression databases

ArrayExpress

Q6QHF9; -.

CleanEx

HS_PAOX; -.

GermOnline

ENSG00000148832; Homo sapiens.

Ontologies

GO:0005777;	Cellular component: peroxisome (inferred from electronic annotation from UniProtKB-KW).
GO:0046592;	Molecular function: polyamine oxidase activity (inferred from electronic annotation from EC).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002937; Amino_oxidase.
Graphical view of domain structure.

Pfam

PF01593; Amino_oxidase; 2.
Pfam graphical view of domain structure.

Genome annotation databases

Ensembl

ENSG00000148832; Homo sapiens. [Contig view]

GeneID

196743; -.

KEGG

hsa:196743; -.

Phylogenomic databases

HOVERGEN

Q6QHF9; -.

Other

NextBio

89545; -.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Cytoplasm; FAD; Flavoprotein; Oxidoreductase; Peroxisome.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`INIT_MET`	`1 1`		`Removed (By similarity).`
`CHAIN`	`2 649`	`648`	`Peroxisomal N(1)-acetyl-spermine/spermidine oxidase.`	`PRO_0000099875`
`MOTIF`	`647 649`	`3`	`Microbody targeting signal (Potential).`
`VAR_SEQ`	`1 267`		`Missing (in isoform 11).`	`VSP_011247`
`VAR_SEQ`	`14 45`		`PRVLVVGGGIAGLGAAQRLCGHSAFPHLRVLE -> GHGPRRGPHPLGALLRWRGGGGRALDPWALPG (in isoform 13).`	`VSP_011261`
`VAR_SEQ`	`23 83`		`IAGLGAAQRLCGHSAFPHLRVLEATARAGGRIRSERCFGG` `VVEVGAHWIHGPSRGNPVFQL -> SSRSCLRGKPHIARFTPRRTGLCCRDGWRPTASSVCGPRR` `CSSPGRGSSWAQPTLFHPCRG (in isoform 12).`	`VSP_011259`
`VAR_SEQ`	`31 168`		`Missing (in isoform 1, isoform 5, isoform 4 and isoform 8).`	`VSP_011249`
`VAR_SEQ`	`46 649`		`Missing (in isoform 13).`	`VSP_011262`
`VAR_SEQ`	`61 80`		`GGVVEVGAHWIHGPSRGNPV -> RVSRTHKLHDGRPAGGHCSF (in isoform 6).`	`VSP_011248`
`VAR_SEQ`	`62 69`		`GVVEVGAH -> AIKDSQTA (in isoform 3).`	`VSP_011250`
`VAR_SEQ`	`70 649`		`Missing (in isoform 3).`	`VSP_011251`
`VAR_SEQ`	`73 489`		`Missing (in isoform 10).`	`VSP_011252`
`VAR_SEQ`	`81 649`		`Missing (in isoform 6).`	`VSP_011256`
`VAR_SEQ`	`84 649`		`Missing (in isoform 12).`	`VSP_011260`
`VAR_SEQ`	`338 649`		`MDLVALAPFGEYTVLPGLDCTFSKGYQGLTNCMMAALPED` `TVVFEKPVKTIHWNGSFQEAAFPGETFPVSVECEDGDRFP` `AHHVIVTVPLGFLREHLDTFFDPPLPAEKAEAIRKIGFGT` `NNKIFLEFEEPFWEPDCQLIQLVWEDTSPLEDAAPELQDA` `WFRKLIGFVVLPAFASVHVLCGFIAGLESEFMETLSDEEV` `LLCLTQVLRRVTGNPRLPAPKSVLRSRWHSAPYTRGSYSY` `VAVGSTGGDLDLLAQPLPADGAGAQLQILFAGEATHRTFY` `STTHGALLSGWREADRLLSLWAPQVQQPRPRL -> TGLCCRDGGRPTASSVCGPRRCSSPGRGSSWAQPTLF` `HPCRG (in isoform 11).`	`VSP_011253`
`VAR_SEQ`	`429 463`		`FLREHLDTFFDPPLPAEKAEAIRKIGFGTNNKIFL -> LSTFSVGSLPDLSLSSWRLCRMKKYFCVSPKCSGE (in isoform 4).`	`VSP_011255`
`VAR_SEQ`	`435 482`		`Missing (in isoform 8).`	`VSP_011254`
`VAR_SEQ`	`464 649`		`Missing (in isoform 4).`	`VSP_011257`
`VAR_SEQ`	`550 649`		`GNPRLPAPKSVLRSRWHSAPYTRGSYSYVAVGSTGGDLDL` `LAQPLPADGAGAQLQILFAGEATHRTFYSTTHGALLSGWR` `EADRLLSLWAPQVQQPRPRL -> APDPVCGGSHTSHVLLHDARGSAVGMEGGRPPPQSVGPAG` `AAAQAEALAGPSLLCSTRVGGRLGPSFLLTDFSLA (in isoform 5).`	`VSP_011258`
`CONFLICT`	`23 23`		`I -> M (in Ref. 2; AAS64378).`
`CONFLICT`	`40 40`		`H -> Q (in Ref. 2; AAS64378).`
`CONFLICT`	`186 186`		`A -> T (in Ref. 2; AAS64379).`
`CONFLICT`	`214 214`		`R -> Q (in Ref. 1; AAN40706).`
`CONFLICT`	`223 223`		`A -> V (in Ref. 1; AAN40706).`
`CONFLICT`	`376 376`		`E -> G (in Ref. 2; AAS64376).`
`CONFLICT`	`396 396`		`E -> K (in Ref. 2; AAS64373).`
`CONFLICT`	`502 502`		`L -> F (in Ref. 2; AAS64381).`
`CONFLICT`	`506 506`		`V -> G (in Ref. 1; AAN40706).`
`CONFLICT`	`551 551`		`N -> S (in Ref. 2; AAS64379).`

Sequence information

Length: 649 AA [This is the length of the unprocessed precursor]

Molecular weight: 70290 Da [This is the MW of the unprocessed precursor]

CRC64: D332E2AA7242F609 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MESTGSVGEA PGGPRVLVVG GGIAGLGAAQ RLCGHSAFPH LRVLEATARA GGRIRSERCF 

        70         80         90        100        110        120 
GGVVEVGAHW IHGPSRGNPV FQLAAEYGLL GEKELSQENQ LVETGGHVGL PSVSYASSGT 

       130        140        150        160        170        180 
SVSLQLVAEM ATLFYGLIDQ TREFLHAAET PVPSVGEYLK KEIGQHVARL CGHSAFPHLR 

       190        200        210        220        230        240 
VLEATARAGG RIRSERCFGG VVEVGAHWIH GPSRGNPVFQ LAAEYGLLGE KELSQENQLV 

       250        260        270        280        290        300 
ETGGHVGLPS VSYASSGASV SLQLVAEMAT LFYGLIDQTR EFLHAAETPV PSVGEYLKKE 

       310        320        330        340        350        360 
IGQHVAGWTE DEETRKLKLA VLNSFFNLEC CVSGTHSMDL VALAPFGEYT VLPGLDCTFS 

       370        380        390        400        410        420 
KGYQGLTNCM MAALPEDTVV FEKPVKTIHW NGSFQEAAFP GETFPVSVEC EDGDRFPAHH 

       430        440        450        460        470        480 
VIVTVPLGFL REHLDTFFDP PLPAEKAEAI RKIGFGTNNK IFLEFEEPFW EPDCQLIQLV 

       490        500        510        520        530        540 
WEDTSPLEDA APELQDAWFR KLIGFVVLPA FASVHVLCGF IAGLESEFME TLSDEEVLLC 

       550        560        570        580        590        600 
LTQVLRRVTG NPRLPAPKSV LRSRWHSAPY TRGSYSYVAV GSTGGDLDLL AQPLPADGAG 

       610        620        630        640 
AQLQILFAGE ATHRTFYSTT HGALLSGWRE ADRLLSLWAP QVQQPRPRL

Q6QHF9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools