[1]	NUCLEOTIDE SEQUENCE [MRNA], AND DEVELOPMENTAL STAGE. DOI=10.1016/j.mce.2003.11.010; PubMed=15026171 [NCBI, ExPASy, EBI, Israel, Japan] Mindnich R., Deluca D., Adamski J.; "Identification and characterization of 17 beta-hydroxysteroid dehydrogenases in the zebrafish, Danio rerio."; Mol. Cell. Endocrinol. 215:19-30(2004).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. STRAIN=AB; NIH - Zebrafish Gene Collection (ZGC) project; Submitted (DEC-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the transformation of estrone (E1) into estradiol (E2), suggesting a central role in estrogen formation (By similarity).
CATALYTIC ACTIVITY: Estradiol-17-beta + NAD(P)⁺ = estrone + NAD(P)H.
PATHWAY: Steroid metabolism; estrogen biosynthesis .
SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Multi-pass membrane protein (By similarity).
DEVELOPMENTAL STAGE: Expressed throughout development. Weakly expressed during early developmental stages from shield to tailbud.
SIMILARITY: Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY551082; AAS58452.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC048053; AAH48053.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC063943; AAH63943.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_957175.1; -.

UniGene

Dr.29406

3D structure databases

ModBase

Q6P3L6.

Organism-specific databases

ZFIN

ZDB-GENE-030131-5628; hsd17b12a.

Gene expression databases

ArrayExpress

Q6P3L6; -.

Ontologies

GO:0005789;	Cellular component: endoplasmic reticulum membrane (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR002198; DHase_sc/Rdtase_SDR.
IPR002347; Glc/ribitol_DHase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

PANTHER

PTHR19410; ADH_short_C2; 1.

Pfam

PF00106; adh_short; 1.
Pfam graphical view of domain structure.

PRINTS

PR00081; GDHRDH.
PR00080; SDRFAMILY.

PROSITE

PS00061; ADH_SHORT; 1.

BLOCKS

Q6P3L6.

Genome annotation databases

Ensembl

ENSDARG00000015709; Danio rerio. [Contig view]

GeneID

327417; -.

KEGG

dre:327417; -.

Phylogenomic databases

HOVERGEN

Q6P3L6; -.

Other

ProtoNet

Q6P3L6.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Endoplasmic reticulum; Lipid synthesis; Membrane; NADP; Oxidoreductase; Steroid biosynthesis; Transmembrane.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 319`	`319`	`Estradiol 17-beta-dehydrogenase 12-A.`	`PRO_0000248371`
`TRANSMEM`	`17 37`	`21`	`Potential.`
`TRANSMEM`	`188 208`	`21`	`Potential.`
`TRANSMEM`	`282 302`	`21`	`Potential.`
`NP_BIND`	`56 85`	`30`	`NADP (By similarity).`
`ACT_SITE`	`208 208`		`Proton acceptor (By similarity).`
`BINDING`	`195 195`		`Substrate (By similarity).`
`CONFLICT`	`187 187`		`K -> E (in Ref. 2; AAH48053).`

Sequence information

Length: 319 AA [This is the length of the unprocessed precursor]

Molecular weight: 35551 Da [This is the MW of the unprocessed precursor]

CRC64: BA0892BF0B7A82C8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MESFNVVETL QPAERALFWV GALITASLAL YVVYKTITGF RIWVLGNGDL LSPKLGKWAV 

        70         80         90        100        110        120 
VTGATDGIGK SYAEELARRG FSMMLISRSQ EKLDDVAKSL ESTYKVETKT IAVDFSQIDV 

       130        140        150        160        170        180 
YPKIEKGLAG LEIGILVNNV GISYSYPEFF LHIPDLENFI TTMINVNITS VCQMTRLVLP 

       190        200        210        220        230        240 
RMEARAKGVI LNISSASGMF PVPLLTIYSS TKAFVDFFSR GLQTEYKCKG IIIQSVLPFF 

       250        260        270        280        290        300 
VATKMTKIRK PTLDKPTPER YVAAELNTVG LQDQTNGYFP HAVMGWVTTI LAPIDLVLNL 

       310 
GLRMNKAQRG GYLRRRKLR

Q6P3L6 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools