NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=ATCC 15356 / HD100 / DSM 50701 / NCIB 9529;
DOI=10.1126/science.1093027; PubMed=14752164 [NCBI, ExPASy, EBI, Israel, Japan]
Rendulic S., Jagtap P., Rosinus A., Eppinger M., Baar C., Lanz C., Keller H., Lambert C., Evans K.J., Goesmann A., Meyer F., Sockett R.E., Schuster S.C.;
"A predator unmasked: life cycle of Bdellovibrio bacteriovorus from a genomic perspective.";
Science 303:689-692(2004).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BX842646; CAE77766.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_967112.1; -.

3D structure databases

ModBase

Q6MRJ2.

Enzyme and pathway databases

BioCyc

BBAC264462:BD0087-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

ProDom

PD003330; GTP_cyclohydroI; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

Q6MRJ2.

Genome annotation databases

2734018; -.

BX842601_GR; Bd0087.

bba:Bd0087; -.

fig|264462.1.peg.82; -.

Phylogenomic databases

HOGENOM

Q6MRJ2; -.

Genome annotation databases

CMR

Q6MRJ2; Bd0087.

Other

ProtoNet

Q6MRJ2.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 170`	`170`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000162962`

Sequence information

Length: 170 AA [This is the length of the unprocessed precursor]

Molecular weight: 19733 Da [This is the MW of the unprocessed precursor]

CRC64: 1531479092C55AED [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKKKNGRDSK ELADFALGES QTNYPETYAP EVLEAFDNKN PGKIAWTTFV CTEFTSLCPK 

        70         80         90        100        110        120 
TRQPDFAKIF INYIADKKMV ESKSLKLYLF SFRNHGDFHE DCVQTICDDL VKLMKPKYIE 

       130        140        150        160        170 
VIGEFTPRGG IAIYPYANYA AKDKFFQELY KKRMSEYAPG KYSMELSKLY

Q6MRJ2 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools