Enoyl-CoA hydratase/Delta(3)-cis-Delta(2)-trans-enoyl-CoA isomerase/3-hydroxybutyryl-CoA epimerase
     (EC 4.2.1.17)
     (EC 5.3.3.8)
     (EC 5.1.2.3)
3-hydroxyacyl-CoA dehydrogenase
     (EC 1.1.1.35)

Gene name

	Name:	fadB
	OrderedLocusNames:	PBPRA0064

From

Photobacterium profundum (Photobacterium sp. (strain SS9))

[TaxID: 74109]

[HAMAP proteome]

Taxonomy

Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Photobacterium.

Protein existence

3: Inferred from homology;

References

[1]

NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1126/science.1103341; PubMed=15746425 [NCBI, ExPASy, EBI, Israel, Japan]
Vezzi A., Campanaro S., D'Angelo M., Simonato F., Vitulo N., Lauro F.M., Cestaro A., Malacrida G., Simionati B., Cannata N., Romualdi C., Bartlett D.H., Valle G.;
"Life at depth: Photobacterium profundum genome sequence and expression analysis.";
Science 307:1459-1461(2005).

Comments

FUNCTION: Catalyzes the formation of an hydroxyacyl-CoA by addition of water on enoyl-CoA. Also exhibits 3-hydroxyacyl-CoA epimerase and 3-hydroxyacyl-CoA dehydrogenase activities (By similarity).
CATALYTIC ACTIVITY: (S)-3-hydroxyacyl-CoA + NAD⁺ = 3-oxoacyl-CoA + NADH.
CATALYTIC ACTIVITY: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H₂O.
CATALYTIC ACTIVITY: (S)-3-hydroxybutanoyl-CoA = (R)-3-hydroxybutanoyl-CoA.
CATALYTIC ACTIVITY: (3Z)-dodec-3-enoyl-CoA = (2E)-dodec-2-enoyl-CoA.
PATHWAY: Lipid metabolism; fatty acid beta-oxidation .
SUBUNIT: Heterotetramer of two alpha chains (fadB) and two beta chains (fadA) (By similarity).
SIMILARITY: In the N-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
SIMILARITY: In the C-terminal section; belongs to the 3-hydroxyacyl-CoA dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR378663; CAG18519.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_128321.1; -.

3D structure databases

ModBase

Q6LW06.

Enzyme and pathway databases

BioCyc

PPRO298386:PBPRA0064-MON; -.

Ontologies

GO:0016507;	Cellular component: fatty acid beta-oxidation multienzyme complex (inferred from electronic annotation from InterPro).
GO:0003857;	Molecular function: 3-hydroxyacyl-CoA dehydrogenase activity (inferred from electronic annotation from HAMAP).
GO:0008692;	Molecular function: 3-hydroxybutyryl-CoA epimerase activity (inferred from electronic annotation from HAMAP).
GO:0050662;	Molecular function: coenzyme binding (inferred from electronic annotation from InterPro).
GO:0004165;	Molecular function: dodecenoyl-CoA delta-isomerase activity (inferred from electronic annotation from HAMAP).
GO:0004300;	Molecular function: enoyl-CoA hydratase activity (inferred from electronic annotation from HAMAP).
GO:0009062;	Biological process: fatty acid catabolic process (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

HAMAP

MF_01621; -; 1.
PBIL [Tree]

InterPro

IPR006180; 3-OHacyl-CoA_DHase_CS.
IPR006176; 3-OHacyl-CoA_DHase_NAD-bd.
IPR006108; 3HC_DHase_C.
IPR001753; Crotonase_core.
IPR013328; DHase_multihelical.
IPR012799; FadB.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.
G3DSA:1.10.1040.10; Opine_DH; 1.

Pfam

PF00725; 3HCDH; 1.
PF02737; 3HCDH_N; 1.
PF00378; ECH; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR02437; FadB; 1.

PROSITE

PS00067; 3HCDH; 1.
PS00166; ENOYL_COA_HYDRATASE; 1.

ProtoNet

Q6LW06.

Genome annotation databases

GeneID

3123934; -.

GenomeReviews

CR354531_GR; PBPRA0064.

KEGG

ppr:PBPRA0064; -.

NMPDR

fig|298386.1.peg.2294; -.

Phylogenomic databases

HOGENOM

Q6LW06; -.

Genome annotation databases

CMR

Q6LW06; PBPRA0064.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Fatty acid metabolism; Isomerase; Lipid degradation; Lipid metabolism; Lyase; Multifunctional enzyme; NAD; Oxidoreductase.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 720`	`720`	`Fatty acid oxidation complex subunit alpha.`	`PRO_0000109272`
`REGION`	`1 189`	`189`	`Enoyl-CoA hydratase/isomerase (By similarity).`
`REGION`	`311 720`	`410`	`3-hydroxyacyl-CoA dehydrogenase (By similarity).`

Sequence information

Length: 720 AA [This is the length of the unprocessed precursor]

Molecular weight: 77647 Da [This is the MW of the unprocessed precursor]

CRC64: 859AC731FF016A06 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MIYQGETLSV RYLDDGIAEL NLNAPGAVNK FDLKTLECLN EALNALYQQS DLKGLLITSD 

        70         80         90        100        110        120 
KDAFIVGADI TEFLGLFAKP AEELSQWLTR ANDIFNKLED LPVPTLSAIN GHALGGGCEC 

       130        140        150        160        170        180 
VLATDFRLAD ITARIGLPET RLGIMPGFGG TVRLPRLLGA DSAMEIITAG KDKKAQDALK 

       190        200        210        220        230        240 
LGLVDAVVAP TALKDAALSM IKDAIAGKLD WQKRRAQKKA PLTLNKIEAT MSFTMAKAMV 

       250        260        270        280        290        300 
AQVAGKHYPA PMTAVIAIEA AARMSRDEAL VVENKHFITL AKTDVAQSLV GIFLNDQYIK 

       310        320        330        340        350        360 
GKAKKAAKEG QPTKKGVVLG AGIMGGGIAY QSALKGVPVL MKDIAVPSLD LGMAEAAKLL 

       370        380        390        400        410        420 
NKQLERGRID GVKMAKVLSG ITPSLHYAGA EDADIVVEAV VENPKIKAAV LAEVESNVSD 

       430        440        450        460        470        480 
TTVIASNTST IPINLLAQSL KRPENFCGMH FFNPVHRMPL VEIIRGEHTS EETISRVVAY 

       490        500        510        520        530        540 
AAKMGKSPIV VNDCPGFFVN RVLFPYFAGF SLLLRDGADF TQVDKVMEKQ FGWPMGPAYL 

       550        560        570        580        590        600 
LDVVGIDTAH HAQAVMAEGF PDRMGKNYKD AVDVMFEQQR FGQKNGNGFF AYSVDRRGKP 

       610        620        630        640        650        660 
KKNVDPAVAE LLAPVLGAAT DFTSDEIIAR MMIPMINEVV RCLEECIIAT PAEADMALVY 

       670        680        690        700        710        720 
GLGFPPFRGG VFRYIDTLGL AEYVAMADKF AHLGAVYEVP TGLREKAAKG ESYYTQQVNA

Q6LW06 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools