FUNCTION: Involved in ds DNA break repair. Has a role in non-homologous integration (NHI) pathways where it is required in the final step of non-homologus end-joining (By similarity).
CATALYTIC ACTIVITY: ATP + (deoxyribonucleotide)(n) + (deoxyribonucleotide)(m) = AMP + diphosphate + (deoxyribonucleotide)(n+m).
SUBCELLULAR LOCATION: Nucleus (By similarity).
SIMILARITY: Belongs to the ATP-dependent DNA ligase family.
SIMILARITY: Contains 2 BRCT domains.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR382130; CAG81303.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

XP_503109.1; -.

3D structure databases

ModBase

Q6C8A3.

Ontologies

GO:0005634;	Cellular component: nucleus (inferred from electronic annotation from UniProtKB-KW).
GO:0005524;	Molecular function: ATP binding (inferred from electronic annotation from InterPro).
GO:0003910;	Molecular function: DNA ligase (ATP) activity (inferred from electronic annotation from InterPro).
GO:0006310;	Biological process: DNA recombination (inferred from electronic annotation from InterPro).
GO:0006281;	Biological process: DNA repair (inferred from electronic annotation from InterPro).
GO:0006260;	Biological process: DNA replication (inferred from electronic annotation from InterPro).
QuickGo view.

Family and domain databases

InterPro

IPR001357; BRCT.
IPR000977; DNA_ligase.
IPR012309; DNA_ligase_A_C.
IPR012310; DNA_ligase_A_M.
IPR012340; NA-bd_OB-fold.
Graphical view of domain structure.

Gene3D

G3DSA:2.40.50.140; OB_NA_bd_sub; 1.

Pfam

PF04679; DNA_ligase_A_C; 1.
PF01068; DNA_ligase_A_M; 1.
Pfam graphical view of domain structure.

SMART

SM00292; BRCT; 2.
SMART graphical view of domain structure.

TIGRFAMs

TIGR00574; dnl1; 1.

PROSITE

PS50172; BRCT; 2.
PS50160; DNA_LIGASE_A3; 1.
PROSITE graphical view of domain structure (profiles).

ProtoNet

Q6C8A3.

Genome annotation databases

GeneID

2911076; -.

KEGG

yli:YALI0D21384g; -.

Phylogenomic databases

HOGENOM

Q6C8A3; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

ATP-binding; Complete proteome; DNA damage; DNA recombination; DNA repair; DNA replication; Ligase; Nucleotide-binding; Nucleus; Repeat.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 956`	`956`	`DNA ligase 4.`	`PRO_0000278386`
`DOMAIN`	`700 793`	`94`	`BRCT 1.`
`DOMAIN`	`857 956`	`100`	`BRCT 2.`
`ACT_SITE`	`309 309`		`N6-AMP-lysine intermediate (By similarity).`

Sequence information

Length: 956 AA [This is the length of the unprocessed precursor]

Molecular weight: 108061 Da [This is the MW of the unprocessed precursor]

CRC64: 5169CFB807F4183B [This is a checksum on the sequence]

        10         20         30         40         50         60 
MSSERRPELE ETAVDPATGS AASRKFSIVQ DAVETTIVAP TNHGPSPRFS TLVRNLFEPL 

        70         80         90        100        110        120 
VNLSAVVAAL RKKPTEAKAH IASQFIKGWV EEVGKDIYPA FRLILPDKDR ERAVYGLKEK 

       130        140        150        160        170        180 
ALGRLWVKVL NLAKDSPDAK ALSEWKQGGN ESAGNFSKRC YEVLSKRTSL TDYGHMTVDE 

       190        200        210        220        230        240 
VNERLDLLAD GETDQAKQIE ILTYFYKHMN ATELKWLVNI ILRQMKMNAT EKVFFEPWHP 

       250        260        270        280        290        300 
DAESLFNVTA SLKRVCWELT DPTKRLTSAE AQVSLFACFM PQIAAFPKYS GQDIAGKHFK 

       310        320        330        340        350        360 
GRPFYIEEKI DGERMQMHMS EYGNKFHWWS RRSKDFTETY GNSLDDASGS LTKRLRGIIN 

       370        380        390        400        410        420 
PKVRNCVLDG EMVAYDPATK KIIPFGTLRT ANRNEQNDLN LTKPMFMVFD ILLLNDKPLV 

       430        440        450        460        470        480 
DYTLAERKRT LRTIFARTDN ETVGQEGVLE VLPYTEATTA AEIETCMRKI IAESSEGLVI 

       490        500        510        520        530        540 
KDPTSVYRVN TRDDSWLKMK PEYMSEFGEK LDVVIIGGYY GSGKRGSILS SYLCGLRADG 

       550        560        570        580        590        600 
SDQFWSFFKV GGGLTAGDYQ AIRTKTEGKW KRWDKNDKPK NVLLAGPNGD LERPDVWIEP 

       610        620        630        640        650        660 
SDSVVVEVKA ASVVASDQYK VGLCLRFPRF RALRLDKTWE DGLTISQFAE LRQTVEMEAE 

       670        680        690        700        710        720 
NKELELEDRK RRNAGPGRGA KRLKLANVSS DEDELGTDER PTSVFKATSF AVLSDMSSPR 

       730        740        750        760        770        780 
YMSKAAVENL IKKHGGTVFQ TVEGPHTIPV ADTRTIKVQA LTKRVHGVDV IRPNWLLDCI 

       790        800        810        820        830        840 
NEEKLVALEP RNLLESSAET LALAKTNVDE FGDSYTRPLT YKEMQEVLRF MDQFDLDQTN 

       850        860        870        880        890        900 
PPDLMMEVLE TNDGAVPKGM LFYGKKVYMS TSNMDTVALE TQFRAYDALR CLQFGGANLV 

       910        920        930        940        950 
TDMKDLVVAV AKTEEEAKEL RRVSSEQVFP FRVVSIKWVE ESWKNGTVEI EDDYPL

Q6C8A3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools