3-beta-hydroxy-Delta(5)-steroid dehydrogenase
     (EC 1.1.1.145)
     (3-beta-hydroxy-5-ene steroid dehydrogenase)
     (Progesterone reductase)
Steroid Delta-isomerase
     (EC 5.3.3.1)
     (Delta-5-3-ketosteroid isomerase)

Gene name

OrderedLocusNames:

FPV046

From

Fowlpox virus (FPV)

[TaxID: 10261]

Taxonomy

Viruses; dsDNA viruses, no RNA stage; Poxviridae; Chordopoxvirinae; Avipoxvirus.

Virus host

Vertebrata [TaxID: 7742]

Protein existence

3: Inferred from homology;

References

[1]	NUCLEOTIDE SEQUENCE [GENOMIC DNA]. STRAIN=FP-9 / Isolate HP-438; PubMed=8077953 [NCBI, ExPASy, EBI, Israel, Japan] Skinner M.A., Moore J.B., Binns M.M., Smith G.L., Boursnell M.E.G.; "Deletion of fowlpox virus homologues of vaccinia virus genes between the 3 beta-hydroxysteroid dehydrogenase (A44L) and DNA ligase (A50R) genes."; J. Gen. Virol. 75:2495-2498(1994).
[2]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. DOI=10.1128/JVI.74.8.3815-3831.2000; PubMed=10729156 [NCBI, ExPASy, EBI, Israel, Japan] Afonso C.L., Tulman E.R., Lu Z., Zsak L., Kutish G.F., Rock D.L.; "The genome of fowlpox virus."; J. Virol. 74:3815-3831(2000).

Comments

FUNCTION: 3-beta-HSD is a bifunctional enzyme, that catalyzes the oxidative conversion of Delta(5)-ene-3-beta-hydroxy steroid, and the oxidative conversion of ketosteroids. The 3-beta-HSD enzymatic system plays a crucial role in the biosynthesis of all classes of hormonal steroids (By similarity).
CATALYTIC ACTIVITY: A 3-beta-hydroxy-Delta⁵-steroid + NAD⁺ = a 3-oxo-Delta⁵-steroid + NADH.
CATALYTIC ACTIVITY: A 3-oxo-Delta⁵-steroid = a 3-oxo-Delta⁴-steroid.
PATHWAY: Lipid metabolism; steroid biosynthesis .
SIMILARITY: Belongs to the 3-beta-HSD family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

Z29716; CAA82802.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
AF198100; AAF44390.1; -; Genomic_DNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

PIR

S41971; S41971.

RefSeq

NP_039008.1; -.

3D structure databases

ModBase

Q67477.

Ontologies

GO:0003854;	Molecular function: 3-beta-hydroxy-delta5-steroid dehydrogenase activity (inferred from electronic annotation from InterPro).
GO:0005488;	Molecular function: binding (inferred from electronic annotation from InterPro).
GO:0004769;	Molecular function: steroid delta-isomerase activity (inferred from electronic annotation from EC).
GO:0006700;	Biological process: C21-steroid hormone biosynthetic process (inferred from electronic annotation from UniProtKB-KW).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR002225; 3Beta_OHSteriod_DHase/Estase.
IPR016040; NAD(P)-bd.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.50.720; NAD(P)-bd; 1.

Pfam

PF01073; 3Beta_HSD; 1.
Pfam graphical view of domain structure.

ProtoNet

Q67477.

Genome annotation databases

GeneID

1486594; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Isomerase; Multifunctional enzyme; NAD; Oxidoreductase; Steroidogenesis.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 370`	`370`	`3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase.`	`PRO_0000087797`
`CONFLICT`	`38 43`		`IRIDQW -> LDRPM (in Ref. 1; CAA82802).`
`CONFLICT`	`251 251`		`N -> K (in Ref. 1; CAA82802).`

Sequence information

Length: 370 AA [This is the length of the unprocessed precursor]

Molecular weight: 42349 Da [This is the MW of the unprocessed precursor]

CRC64: 730FE72BA84FA681 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MRTLVYVVTG GCGFLGRHII NNLILFESSL KEVRVYDIRI DQWLLDLVEK CNIIKIVPVI 

        70         80         90        100        110        120 
GDVRNKSTLD EALRSADVVI HIASINDVAG KFTNDSIMDV NINGTKNVVD SCLYNGVRVL 

       130        140        150        160        170        180 
VYTSSYSAVG PNFLGDAMIR GNENTYYQSN HKEAYPLSKQ LSEKYILEAN GTMSNIGLRL 

       190        200        210        220        230        240 
CTCALRPLGV FGEYCPVLET LYRRSYKSRK MYKYADDKVF HSRVYAGNVA WMHILAARNM 

       250        260        270        280        290        300 
IENGQHSPLC NNVYYCYDTS PTEHYHDFNM HFFNQLGMDL RNTCLPLWCL RFIANINKGL 

       310        320        330        340        350        360 
RVLLSPICSY TPLLNPYTLI KECTTFTIET DKAFKDFGYV PLYTWEESRS KTQLWIRELE 

       370 
AKSSSQKPKS

Q67477 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools