[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. TISSUE=Kidney; DOI=10.1101/gr.2596504; PubMed=15489334 [NCBI, ExPASy, EBI, Israel, Japan] The MGC Project Team; "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."; Genome Res. 14:2121-2127(2004).
[2]	PROTEIN SEQUENCE OF 55-75; 162-174; 327-340 AND 444-453, AND MASS SPECTROMETRY. STRAIN=Sprague-Dawley; TISSUE=Hippocampus, and Spinal cord; Lubec G., Afjehi-Sadat L., Chen W.-Q.; Submitted (APR-2007) to UniProtKB.

Comments

FUNCTION: ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation (By similarity).
CATALYTIC ACTIVITY: An aldehyde + NAD⁺ + H₂O = an acid + NADH.
PATHWAY: Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2 .
SUBUNIT: Homotetramer (By similarity).
SUBCELLULAR LOCATION: Mitochondrion matrix (By similarity).
SIMILARITY: Belongs to the aldehyde dehydrogenase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC081884; AAH81884.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001011975.1; -.

UniGene

Rn.12547

3D structure databases

SMR

Q66HF8; 26-519.

ModBase

Q66HF8.

Organism-specific databases

RGD

1306737; Aldh1b1.

Gene expression databases

ArrayExpress

Q66HF8; -.

Ontologies

GO:0005759;	Cellular component: mitochondrial matrix (inferred from electronic annotation from UniProtKB-SubCell).
QuickGo view.

Family and domain databases

InterPro

IPR016160; Ald_DHase_CS.
IPR016162; Ald_DHase_N.
IPR015590; Aldehyde_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.605.10; Aldehyde_dehydrogenase_N; 1.

PANTHER

PTHR11699; Aldehyde_dehyd; 1.

Pfam

PF00171; Aldedh; 1.
Pfam graphical view of domain structure.

PROSITE

PS00070; ALDEHYDE_DEHYDR_CYS; 1.
PS00687; ALDEHYDE_DEHYDR_GLU; 1.

BLOCKS

Q66HF8.

Genome annotation databases

Ensembl

ENSRNOG00000011497; Rattus norvegicus. [Contig view]

GeneID

298079; -.

KEGG

rno:298079; -.

Phylogenomic databases

HOVERGEN

Q66HF8; -.

Other

ProtoNet

Q66HF8.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Direct protein sequencing; Mitochondrion; NAD; Oxidoreductase; Transit peptide.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 19`	`19`	`Mitochondrion (Potential).`
`CHAIN`	`20 519`	`500`	`Aldehyde dehydrogenase X, mitochondrial.`	`PRO_0000271413`
`NP_BIND`	`264 269`	`6`	`NAD (By similarity).`
`ACT_SITE`	`287 287`		`Proton acceptor (By similarity).`
`ACT_SITE`	`321 321`		`Nucleophile (By similarity).`
`SITE`	`188 188`	`1`	`Transition state stabilizer (By similarity).`

Sequence information

Length: 519 AA [This is the length of the unprocessed precursor]

Molecular weight: 57625 Da [This is the MW of the unprocessed precursor]

CRC64: 6D25E0E806F8EA50 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLNARFLVPR LLCLQGRTTS YSTAAALPNP IPNPEIRYNQ LFINNEWHDA VSKKTFPTVN 

        70         80         90        100        110        120 
PTTGEVIGHV AEGDRADVDL AVRAAREAFR LGSPWRRMDA SERGRLLNRL ADLVERDRVY 

       130        140        150        160        170        180 
LASLETLDNG KPFQESYVLD LDEVIKVYRY LAGWADKWHG KTIPMDGEHF CFTRHEPVGV 

       190        200        210        220        230        240 
CGQIIPWNFP LVMQGWKLAP ALATGNTVVM KVAEQTPLSA LYLASLIKEA GFPPGVVNII 

       250        260        270        280        290        300 
TGYGPTAGAA IAQHMDVDKV AFTGSTEVGH LIQKAAGDSN LKRVTLELGG KSPSIVLADA 

       310        320        330        340        350        360 
DMDHAVDQCH EALFFNMGQC CCAGSRTFVE ESIYHEFLER TVEKAKKRKV GNPFELDTQQ 

       370        380        390        400        410        420 
GPQVDKEQFE KILGYIRLGQ KEGAKLLCGG ERFGERGFFI KPTVFGNVQD DMRIAREEIF 

       430        440        450        460        470        480 
GPVQPLFKFK KIEEVIQRAN NTRYGLAAAV FTRDLDKALY FSQALQAGTV WVNTYNIVTC 

       490        500        510 
HTPFGGFKES GNGRELGEDG LKAYTEVKTV TIKVSEKNS

Q66HF8 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools