NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=YCH46;
DOI=10.1073/pnas.0404172101; PubMed=15466707 [NCBI, ExPASy, EBI, Israel, Japan]
Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.;
"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation.";
Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AP006841; BAD48217.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_098751.1; -.

3D structure databases

ModBase

Q64WA9.

Enzyme and pathway databases

BioCyc

BFRA295405:BF1466-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0033739;	Molecular function: queuine synthase activity (inferred from electronic annotation from EC).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

Q64WA9.

Genome annotation databases

GeneID

3082339; -.

GenomeReviews

AP006841_GR; BF1466.

KEGG

bfr:BF1466; -.

Phylogenomic databases

HOGENOM

Q64WA9; -.

Genome annotation databases

CMR

Q64WA9; BF1466.

Other

ProtoNet

Q64WA9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 151`	`151`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000162959`

Sequence information

Length: 151 AA [This is the length of the unprocessed precursor]

Molecular weight: 17708 Da [This is the MW of the unprocessed precursor]

CRC64: 7C7AAF7B1F0282D8 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MTELKEQLSL LGRKTEYKQD YAPEVLEAFD NKHPENDYWV RFNCPEFTSL CPITGQPDFA 

        70         80         90        100        110        120 
EIRISYLPDV KMVESKSLKL YLFSFRNHGA FHEDCVNIIM KDLIRLMDPK YIEVTGIFTP 

       130        140        150 
RGGISIYPYA NYGRPGTKYE EMATHRLMNH E

Q64WA9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools