[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). TISSUE=Embryo; NIH - Xenopus Gene Collection (XGC) project; Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Histone demethylase that specifically demethylates 'Lys-36' of histone H3, thereby playing a central role in histone code. Preferentially demethylates dimethylated H3 'Lys-36' residue while it has weak or no activity for mono- and tri-methylted H3 'Lys-36' (By similarity).
CATALYTIC ACTIVITY: Protein N₆,N⁶-dimethyl-L-lysine + 2-oxoglutarate + O₂ = protein N⁶-methyl-L-lysine + succinate + formaldehyde + CO₂.
CATALYTIC ACTIVITY: Protein N⁶-methyl-L-lysine + 2-oxoglutarate + O₂ = protein L-lysine + succinate + formaldehyde + CO₂.
COFACTOR: Binds 1 Fe(2+) ion per subunit (By similarity).
SUBCELLULAR LOCATION: Nucleus (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	Q640I9-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	Q640I9-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_017479, VSP_017480.

DOMAIN: The JmjC domain mediates the demethylation activity (By similarity).
SIMILARITY: Belongs to the JHDM1 histone demethylase family.
SIMILARITY: Contains 1 CXXC-type zinc finger.
SIMILARITY: Contains 1 F-box domain.
SIMILARITY: Contains 1 JmjC domain.
SIMILARITY: Contains 4 LRR (leucine-rich) repeats.
SIMILARITY: Contains 1 PHD-type zinc finger.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

BC073414; AAH73414.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
BC082636; AAH82636.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001085840.1; -.

UniGene

Xl.52422

3D structure databases

SMR

Q640I9; 35-361.

ModBase

Q640I9.

Ontologies

GO:0051864;	Molecular function: histone demethylase activity (H3-K36 specific) (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001810; F-box.
IPR013129; TF_JmjC.
IPR003347; TF_JmjC_AAH.
IPR002857; Znf_CXXC.
IPR001965; Znf_PHD.
Graphical view of domain structure.

Pfam

PF00646; F-box; 1.
PF02373; JmjC; 1.
PF02008; zf-CXXC; 1.
Pfam graphical view of domain structure.

SMART

SM00558; JmjC; 1.
SM00249; PHD; 1.
SMART graphical view of domain structure.

PROSITE

PS50181; FBOX; FALSE_NEG.
PS51184; JMJC; 1.
PS51058; ZF_CXXC; 1.
PS01359; ZF_PHD_1; FALSE_NEG.
PS50016; ZF_PHD_2; 1.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q640I9.

Genome annotation databases

GeneID

444267; -.

KEGG

xla:444267; -.

Phylogenomic databases

HOVERGEN

Q640I9; -.

Other

ProtoNet

Q640I9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Chromatin regulator; Coiled coil; Dioxygenase; Iron; Leucine-rich repeat; Metal-binding; Nucleus; Oxidoreductase; Phosphoprotein; Repeat; Transcription; Transcription regulation; Zinc; Zinc-finger.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 1259`	`1259`	`JmjC domain-containing histone demethylation protein 1B.`	`PRO_0000226786`
`DOMAIN`	`147 315`	`169`	`JmjC.`
`DOMAIN`	`985 1030`	`46`	`F-box.`
`REPEAT`	`1071 1094`	`24`	`LRR 1.`
`REPEAT`	`1135 1158`	`24`	`LRR 2.`
`REPEAT`	`1192 1216`	`25`	`LRR 3.`
`REPEAT`	`1217 1259`	`43`	`LRR 4.`
`ZN_FING`	`567 613`	`47`	`CXXC-type.`
`ZN_FING`	`620 686`	`67`	`PHD-type.`
`COILED`	`864 891`	`28`	`Potential.`
`COMPBIAS`	`688 799`	`112`	`Lys-rich.`
`METAL`	`211 211`		`Iron (catalytic) (By similarity).`
`METAL`	`213 213`		`Iron (catalytic) (By similarity).`
`METAL`	`283 283`		`Iron (catalytic) (By similarity).`
`BINDING`	`208 208`		`Substrate (By similarity).`
`BINDING`	`228 228`		`Substrate (By similarity).`
`VAR_SEQ`	`1 521`		`Missing (in isoform 2).`	`VSP_017479`
`VAR_SEQ`	`522 539`		`NLAISGVPVVMWPKKPSK -> MALSLSNDDEEYDSEQEQ (in isoform 2).`	`VSP_017480`
`CONFLICT`	`919 919`		`N -> H (in Ref. 1; AAH73414).`

Sequence information

Length: 1259 AA [This is the length of the unprocessed precursor]

Molecular weight: 144054 Da [This is the MW of the unprocessed precursor]

CRC64: 8FAAAE440E4765C3 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MEAKKDSARR LRSIDRTRYA EHEDLSDVED MVSIRGFSLE QKVSCNAYRG NFVRYMEGKD 

        70         80         90        100        110        120 
FTYEYVQREA LQTPLLFSTK EGLGIKMPEQ DFTVRDVKLL VGSRRVVDVM DVNTQKSIDM 

       130        140        150        160        170        180 
SMSQFVRYYE TPEMEREKLY NVISLEFSHT KLERVVKRPT VVDAVDWVDN MWPRHLKEQQ 

       190        200        210        220        230        240 
KESTNVIAEM KYPKVKKYCL MSVKGCYTDF HIDFGGTSVW YHVFRGGKVF WLIPPTGHNL 

       250        260        270        280        290        300 
QLYEEWLLSG KQTDIFLGDR SEGCQRIELK QGQTFFIPSG WIHAVYTPAD SLVFGGNILH 

       310        320        330        340        350        360 
SFNIPMQLRV FEIEDRTRVN AKFRYPFYYE MCWYVLERYV CTLTKRSHLI KEYQRESMIT 

       370        380        390        400        410        420 
DRKPSLDSHS SDSWLEMEEE SCDIHIKEEK GNLVEKPSKQ SGDESSTTNS THSNGKDAAE 

       430        440        450        460        470        480 
KKQKATLMQQ LKRTLSNDSD ESSKSIVHSD FPKTPTGSPA TDMPCKLTHL TEFELKGLKA 

       490        500        510        520        530        540 
LVEKLESLPE NKKCVPEGIE DPQALLEDMK TVLKEHADDD NNLAISGVPV VMWPKKPSKN 

       550        560        570        580        590        600 
RAVGRPKGKI ASSPAVKLSA NRTSAGARRR RTRCRKCEAC LRTECGECHF CKDMKKFGGP 

       610        620        630        640        650        660 
GRMKQSCIMR QCIAPVLPHT AVCLVCGEAG KEDCVEGQEA KFNVMLMECS ICNEIIHPGC 

       670        680        690        700        710        720 
LKTKESDGVV NDELPNCWEC PKCNHAGKTG KVYKQKRGPG FKYASNLPGS LLKEQKVNRD 

       730        740        750        760        770        780 
NKELTEIVKK KVEREETPKQ IPEEQPKKKP TEGIIKKKPE DGHVRKKRKF EKPPDPSVRK 

       790        800        810        820        830        840 
RLKLVKEEKL LRKKRRSWKT PDERAMMAKT LRRIKQEPDD DLTEAAPKAK ESDQSRSSSP 

       850        860        870        880        890        900 
TAGPSTEGSE PKEKKKIRRK RRVSNKELSK ELSKELNQEI QKTESSLASE NHHPIKSEPE 

       910        920        930        940        950        960 
SDNEESKKCI STNGERMHNF SKGLNGTPKE LRHELFPSLK TTPRANNRPP PSLSPPKCMQ 

       970        980        990       1000       1010       1020 
MERHVIRPPP ISPPPDSLPL ANGAAHVMQR EVWMAIFSYL SHRDLCICMR ICRTWNRWCC 

      1030       1040       1050       1060       1070       1080 
DKRLWTQIDL NRCKSITPLM LSGIIRRQPA SLDLSWTNIS KKQLSWLINR LPALRDLNLS 

      1090       1100       1110       1120       1130       1140 
GCSWIAVSAL CSSCCPLLRT LNVQWVEGLK DAQMRDLLSP PTDNRPGQID NRSKLRNITE 

      1150       1160       1170       1180       1190       1200 
LRLAGLDITD ASLRLMIRHM PLLAKLDLSY CNHVTDQSIN LLTAVGTSTR DTLLEMNLSD 

      1210       1220       1230       1240       1250 
CNNVTDQCLT FFKRCGNICL IDLRFCKQVS KESCEQFIAE MSVIVQFGQT EEKLLQKVS

Q640I9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools