NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
STRAIN=CB;
TISSUE=Bursa of Fabricius;
DOI=10.1186/gb-2004-6-1-r6; PubMed=15642098 [NCBI, ExPASy, EBI, Israel, Japan]
Caldwell R.B., Kierzek A.M., Arakawa H., Bezzubov Y., Zaim J., Fiedler P., Kutter S., Blagodatski A., Kostovska D., Koter M., Plachy J., Carninci P., Hayashizaki Y., Buerstedde J.-M.;
"Full-length cDNAs from chicken bursal lymphocytes to facilitate gene function analysis.";
Genome Biol. 6:RESEARCH006.1-RESEARCH006.9(2005).

Comments

FUNCTION: Catalyzes the post-translational formation of 4-hydroxyproline in -Xaa-Pro-Gly- sequences in collagens and other proteins (By similarity).
CATALYTIC ACTIVITY: Procollagen L-proline + 2-oxoglutarate + O₂ = procollagen trans-4-hydroxy-L-proline + succinate + CO₂.
COFACTOR: Iron (By similarity).
COFACTOR: Ascorbate (By similarity).
SUBUNIT: Heterotetramer of two alpha-2 chains and two beta chains (the beta chain is the multi-functional PDI) (By similarity).
SUBCELLULAR LOCATION: Endoplasmic reticulum lumen (By similarity).
SIMILARITY: Belongs to the P4HA family.
SIMILARITY: Contains 1 PKHD (prolyl/lysyl hydroxylase) domain.
SIMILARITY: Contains 1 TPR repeat.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AJ719729; CAG31388.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001006155.1; -.

UniGene

Gga.5799

3D structure databases

ModBase

Q5ZLK5.

Ontologies

GO:0005788;	Cellular component: endoplasmic reticulum lumen (inferred from electronic annotation from UniProtKB-SubCell).
GO:0004656;	Molecular function: procollagen-proline 4-dioxygenase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR005123; 2OG-FeII_Oase.
IPR006620; Pro_4_hyd_alph.
IPR013547; Pro_4_hyd_alph_N.
IPR011990; TPR-like_helical.
IPR013026; TPR_region.
Graphical view of domain structure.

Gene3D

G3DSA:1.25.40.10; TPR-like_helical; 1.

Pfam

PF03171; 2OG-FeII_Oxy; 1.
PF08336; P4Ha_N; 1.
Pfam graphical view of domain structure.

SMART

SM00702; P4Hc; 1.
SMART graphical view of domain structure.

PROSITE

PS50005; TPR; FALSE_NEG.
PS50293; TPR_REGION; FALSE_NEG.
PROSITE graphical view of domain structure (profiles).

BLOCKS

Q5ZLK5.

Genome annotation databases

Ensembl

ENSGALG00000006677; Gallus gallus. [Contig view]

GeneID

416326; -.

KEGG

gga:416326; -.

Phylogenomic databases

HOVERGEN

Q5ZLK5; -.

Other

ProtoNet

Q5ZLK5.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Dioxygenase; Endoplasmic reticulum; Glycoprotein; Iron; Metal-binding; Oxidoreductase; Signal; TPR repeat; Vitamin C.

Features

Feature table viewer

Feature aligner

`Key`	`From To`	`Length`	`Description`	`FTId`
`SIGNAL`	`1 21`	`21`	`Potential.`
`CHAIN`	`22 534`	`513`	`Prolyl 4-hydroxylase subunit alpha-2.`	`PRO_0000041836`
`REPEAT`	`207 240`	`34`	`TPR.`
`DOMAIN`	`337 518`	`182`	`PKHD.`
`ACT_SITE`	`510 510`		`Potential.`
`METAL`	`431 431`		`Iron (By similarity).`
`METAL`	`433 433`		`Iron (By similarity).`
`METAL`	`500 500`		`Iron (By similarity).`
`CARBOHYD`	`115 115`		`N-linked (GlcNAc...) (Potential).`
`CARBOHYD`	`263 263`		`N-linked (GlcNAc...) (Potential).`

Sequence information

Length: 534 AA [This is the length of the unprocessed precursor]

Molecular weight: 61435 Da [This is the MW of the unprocessed precursor]

CRC64: F3176919EBDEEBB1 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MKPWLCLVFF TSAFLIWHAE AEFFTSIGQM TDLIYAEKDL VQSLKEYIRA EETKLSQIKS 

        70         80         90        100        110        120 
WAEKMDVLTS KSTSDPEGYL AHPVNAYKLV KRLNTDWLEL ENLVLQDTTN GFITNLTIQR 

       130        140        150        160        170        180 
QFFPTEEDET GAAKALMRLQ DTYKLDPETL SRGNLPGTKY RSSLTVSDCF GMGKTAYNDG 

       190        200        210        220        230        240 
DYYHTVLWME QALKQHDEGE DTTVSKVEIL DYLSYAVFQF GDLHRAMELT RRLISLDSTH 

       250        260        270        280        290        300 
ERAGSNLRYF EKLLEKEREK PSNKTVATTE PVVQSGAYER PLDYLPERDI YEALCRGEGV 

       310        320        330        340        350        360 
KMTPRRQKRL FCRYHDGNRN PHLLIAPFKE EDEWDSPHIV RYYDVMSDEE IEKIKQLAKP 

       370        380        390        400        410        420 
KLARATVRDP KTGVLTVASY RVSKSSWLEE DDDPVVAKVN QRMQQITGLT VKTAELLQVA 

       430        440        450        460        470        480 
NYGMGGQYEP HFDFSRRPFD STLKSEGNRL ATFLNYMSDV EAGGATVFPD FGAAIWPKKG 

       490        500        510        520        530 
TAVFWYNLFR SGEGDYRTRH AACPVLVGCK WVSNKWFHER GNEFLRPCGR TEVD

Q5ZLK5 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools