[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Takaki Y., Kageyama Y., Shimamura S., Suzuki H., Nishi S., Hatada Y., Kawai S., Ito S., Horikoshi K.; "The complete genome sequence of the alkaliphilic Bacillus clausii KSM-K16."; Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 1 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AP006627; BAD64661.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_175622.1; -.

3D structure databases

ModBase

Q5WG44.

Enzyme and pathway databases

BioCyc

BCLA66692:ABC2126-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00818; -; 1.
PBIL [Tree]

InterPro

IPR016856; CN_OxRdtase_NADPH-dep_QueF.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF027377; Nitrile_oxidored_QueF; 1.

ProDom

PD003330; GTP_cyclohydroI; 1.
[Domain structure / List of seq. sharing at least 1 domain]

TIGRFAMs

TIGR03139; QueF-II; 1.

BLOCKS

Q5WG44.

Genome annotation databases

GeneID

3203664; -.

GenomeReviews

AP006627_GR; ABC2126.

KEGG

bcl:ABC2126; -.

NMPDR

fig|66692.3.peg.2430; -.

Phylogenomic databases

HOGENOM

Q5WG44; -.

Genome annotation databases

CMR

Q5WG44; ABC2126.

Other

ProtoNet

Q5WG44.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 165`	`165`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000162954`

Sequence information

Length: 165 AA [This is the length of the unprocessed precursor]

Molecular weight: 19531 Da [This is the MW of the unprocessed precursor]

CRC64: 2ADAD4A73579A0CF [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAGRQEKELQ NVTLLGSEQT EYKYSYDPRV LETFDNQHPY RDYMVKFNCP EFTTLCPKTG 

        70         80         90        100        110        120 
QPDFATLYIS YIPEQKMVES KSLKLYLFSF RNHGDFHEDS VNTIMNDLIE LMDPRYIEVW 

       130        140        150        160 
GKFTPRGGIS IDPYCNYGKK GTKYEEIANY RLMNHDLYPE TITNR

Q5WG44 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools