NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
STRAIN=Rowbotham-Bradford;
DOI=10.1126/science.1101485; PubMed=15486256 [NCBI, ExPASy, EBI, Israel, Japan]
Raoult D., Audic S., Robert C., Abergel C., Renesto P., Ogata H., La Scola B., Susan M., Claverie J.-M.;
"The 1.2-megabase genome sequence of Mimivirus.";
Science 306:1344-1350(2004).

Comments

FUNCTION: Catalyzes the 14-alpha demethylation of obtusifoliol to 4 alpha-methyl-5 alpha-ergosta-8,14,24(28)-trien-3 beta-ol (By similarity).
CATALYTIC ACTIVITY: Obtusifoliol + 3 O₂ + 3 NADPH = 4-alpha-methyl-5-alpha-ergosta-8,14,24²⁸-trien-3-beta-ol + formate + 3 NADP⁺ + 4 H₂O.
COFACTOR: Heme group (By similarity).
PATHWAY: Steroid metabolism; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6 .
SUBCELLULAR LOCATION: Membrane (Potential).
SIMILARITY: Belongs to the cytochrome P450 family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY653733; AAV51068.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_143162.1; -.

3D structure databases

ModBase

Q5UQI3.

Ontologies

GO:0008398;	Molecular function: sterol 14-demethylase activity (inferred from electronic annotation from EC).
QuickGo view.

Family and domain databases

InterPro

IPR001128; Cyt_P450.
IPR002403; Cyt_P450_E_grp-IV.
Graphical view of domain structure.

Gene3D

G3DSA:1.10.630.10; Cyt_P450; 1.

PANTHER

PTHR19383; Cyt_P450; 1.

Pfam

PF00067; p450; 1.
Pfam graphical view of domain structure.

PRINTS

PR00465; EP450IV.

PROSITE

PS00086; CYTOCHROME_P450; 1.

BLOCKS

Q5UQI3.

Genome annotation databases

GeneID

3162320; -.

Other

ProtoNet

Q5UQI3.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Heme; Iron; Lipid synthesis; Membrane; Metal-binding; Methyltransferase; Monooxygenase; NADP; Oxidoreductase; Steroid biosynthesis; Sterol biosynthesis; Transferase.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`CHAIN`	`1 709`	`709`	`Probable cytochrome P450 51.`	`PRO_0000052015`
`METAL`	`425 425`		`Iron (heme axial ligand) (By similarity).`

Sequence information

Length: 709 AA [This is the length of the unprocessed precursor]

Molecular weight: 82186 Da [This is the MW of the unprocessed precursor]

CRC64: FCE6AF553DEF2F6F [This is a checksum on the sequence]

        10         20         30         40         50         60 
MLFELSIGAI IGFLTLYLLK RFNESKNFIT PDNLKKIPIV EGAVPVLGHG PAFSKDIMQF 

        70         80         90        100        110        120 
MKNCYKKYGS VFQLKIFRTN MVVLCDRKLS EEFFKSREND MSLYDVLNRL FFGLAFSDKP 

       130        140        150        160        170        180 
DSLEFIIKMV KKTITIRYDD FAPKIMDEAQ RLTKIMRESH SGKKLDMIPE IIKFVSRTSA 

       190        200        210        220        230        240 
RCFIAMDIDD EFYDALNKFT NLLNKIVVLT YFVPHWLLNA TLNRFMLRKY RMRMTKLLEN 

       250        260        270        280        290        300 
EIEKYRTDLN KSDSLLFRKC VDHIDPETGA TLTNQDIGDI VVCLLYVSSE NTSLLATNCL 

       310        320        330        340        350        360 
IDLTLNPKYW DLIKSECSAM IALGDYKNLF KAPLLNSIVM ESARLNSHVF ALARKPKTVN 

       370        380        390        400        410        420 
RIGDYFVADN VDTISLCEPA LMKFEIASDV YANPNSYDPV RFMAPRNEPK DSGHVMNWGK 

       430        440        450        460        470        480 
GVHECPGKQF AIYEVKAAIA YIVTNFERFE FNHNDLKINY FSPSAMCEKN ISVEFIPSQQ 

       490        500        510        520        530        540 
NIHNIVYKDR TYIVEHIKCN ETSAWLIYNA LDRQQQREYY QYTYEISTDS QEHKLIEKAG 

       550        560        570        580        590        600 
PHKPFPIAYD KLVYTGQSNC MTPTKWYDFA SDIWELLTEN YAELGFPIYD DKIRNFVPNS 

       610        620        630        640        650        660 
FYGQLYSVES IMPTHRDQHV DYGLSISIGS NCEFVIEDKT ILLPSGSVLI GDFSKISHSV 

       670        680        690        700 
SKIFHEKPDH LSDFEFFNRV RFSAQIRSID PDVQPLMTTQ EFLDMISEY

Q5UQI3 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools