[1]	NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). TISSUE=Heart; The German cDNA consortium; Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.

Comments

CATALYTIC ACTIVITY: Isocitrate + NAD⁺ = 2-oxoglutarate + CO₂ + NADH.
COFACTOR: Binds 1 magnesium or manganese ion per subunit (By similarity).
SUBUNIT: Heterooligomer of subunits alpha, beta, and gamma in the apparent ratio of 2:1:1 (By similarity).
SUBCELLULAR LOCATION: Mitochondrion (By similarity).

ALTERNATIVE PRODUCTS: 2 named isoforms [FASTA] produced by alternative splicing.

Name	1
Isoform ID	Q5RBT4-1
This is the isoform sequence displayed in this entry.

Name	2
Isoform ID	Q5RBT4-2
Note: No experimental confirmation available.
Features which should be applied to build the isoform sequence: VSP_014101.

SIMILARITY: Belongs to the isocitrate and isopropylmalate dehydrogenases family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR858549; CAH90776.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]
CR859803; CAH91961.1; -; mRNA.	[EMBL / GenBank / DDBJ] [CoDingSequence]

3D structure databases

ModBase

Q5RBT4.

Family and domain databases

InterPro

IPR004434; IsoCit_DHase_NAD_mit.
IPR001804; IsoCit_IM_DHase.
Graphical view of domain structure.

Gene3D

G3DSA:3.40.718.10; IDH_IMDH; 1.

PANTHER

PTHR11835; IDH_IMDH_dimeric; 1.

Pfam

PF00180; Iso_dh; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00175; mito_nad_idh; 1.

PROSITE

PS00470; IDH_IMDH; 1.

BLOCKS

Q5RBT4.

Phylogenomic databases

HOVERGEN

Q5RBT4; -.

Other

ProtoNet

Q5RBT4.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Alternative splicing; Magnesium; Manganese; Metal-binding; Mitochondrion; NAD; Oxidoreductase; Transit peptide; Tricarboxylic acid cycle.

Features

Feature table viewer

`Key`	`From To`	`Length`	`Description`	`FTId`
`TRANSIT`	`1 34`	`34`	`Mitochondrion (By similarity).`
`CHAIN`	`35 385`	`351`	`Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial.`	`PRO_0000014446`
`METAL`	`251 251`		`Magnesium or manganese (By similarity).`
`BINDING`	`133 133`		`Substrate (By similarity).`
`BINDING`	`164 164`		`Substrate (By similarity).`
`BINDING`	`251 251`		`Substrate (By similarity).`
`SITE`	`218 218`	`1`	`Critical for catalysis (By similarity).`
`VAR_SEQ`	`367 385`		`STTTDFIKSVIGHLHPHGS -> ATCHDFTEAVIAALPHP (in isoform 2).`	`VSP_014101`
`CONFLICT`	`171 171`		`C -> Y (in Ref. 1; CAH90776).`
`CONFLICT`	`361 361`		`R -> S (in Ref. 1; CAH91961).`

Sequence information

Length: 385 AA [This is the length of the unprocessed precursor]

Molecular weight: 42166 Da [This is the MW of the unprocessed precursor]

CRC64: 6341167AD11A7615 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MAAVSGVRWL TRALVSAGNP GAWRGLSTSA AAHAASRSQA EDVRVEGSFP VTMLPGDGVG 

        70         80         90        100        110        120 
PELMHAVKEV FKAAAVPVEF QEHHLSEVQN MASEEKLEQV LSSMKENKVA IIGKIHTPME 

       130        140        150        160        170        180 
YKGELASYDM RLRRKLDLFA NVVHVKSLPG YMTRHNNLDL VIIREQTEGE CSSLEHESAR 

       190        200        210        220        230        240 
GVIECLKIVT RAKSQRIAKF AFDYATKKGR SKVIAVHKAN IMKLGDGLFL QCCEEVAELY 

       250        260        270        280        290        300 
PKIKFETMII DNCCMQLVQN PYQFDVLVMP NLYGNIIDNL AAGLVGGAGV VPGESYSAEY 

       310        320        330        340        350        360 
AVFETGARHP FAQAVGRNIA NPTAMLLSAS NMLRHLNLEY HSSMIADAVK KVIKVGKVRT 

       370        380 
RDMGGYSTTT DFIKSVIGHL HPHGS

Q5RBT4 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
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	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools