NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
DOI=10.1007/s00203-004-0742-9; PubMed=15551059 [NCBI, ExPASy, EBI, Israel, Japan]
Rabus R., Kube M., Heider J., Beck A., Heitmann K., Widdel F., Reinhardt R.;
"The genome sequence of an anaerobic aromatic-degrading denitrifying bacterium, strain EbN1.";
Arch. Microbiol. 183:27-36(2005).

Comments

FUNCTION: Catalyzes the NADPH-dependent reduction of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) (By similarity).
CATALYTIC ACTIVITY: 7-aminomethyl-7-carbaguanine + 2 NADP⁺ = 7-cyano-7-carbaguanine + 2 NADPH.
PATHWAY: tRNA modification; queuosine-tRNA biosynthesis .
SUBCELLULAR LOCATION: Cytoplasm (Probable).
SIMILARITY: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

CR555306; CAI07092.1; -; Genomic_DNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

YP_157993.1; -.

3D structure databases

ModBase

Q5P6G9.

Enzyme and pathway databases

BioCyc

ASP76114:EBA1782-MON; -.

Ontologies

GO:0005737;	Cellular component: cytoplasm (inferred from electronic annotation from HAMAP).
GO:0046857;	Molecular function: oxidoreductase activity, acting on other nitrogenous compounds as donors, with NAD or NADP as acceptor (inferred from electronic annotation from HAMAP).
GO:0008616;	Biological process: queuosine biosynthetic process (inferred from electronic annotation from HAMAP).
QuickGo view.

Family and domain databases

HAMAP

MF_00817; -; 1.
PBIL [Tree]

InterPro

IPR016428; CN_OxRdtase_NADPH-dep_YqcD.
IPR001474; GTP_CycOHase_I.
Graphical view of domain structure.

Pfam

PF01227; GTP_cyclohydroI; 1.
Pfam graphical view of domain structure.

PIRSF

PIRSF004750; Nitrile_oxidored_YqcD_prd; 1.

TIGRFAMs

TIGR03138; QueF; 1.

BLOCKS

Q5P6G9.

Genome annotation databases

GeneID

3182983; -.

GenomeReviews

CR555306_GR; AZOSEA09670.

KEGG

eba:ebA1782; -.

NMPDR

fig|76114.4.peg.484; -.

Phylogenomic databases

HOGENOM

Q5P6G9; -.

Genome annotation databases

CMR

Q5P6G9; AZOSEA09670.

Other

ProtoNet

Q5P6G9.

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Complete proteome; Cytoplasm; NADP; Oxidoreductase; Queuosine biosynthesis.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 283`	`283`		`NADPH-dependent 7-cyano-7-deazaguanine reductase.`	`PRO_0000163019`

Sequence information

Length: 283 AA [This is the length of the unprocessed precursor]

Molecular weight: 32063 Da [This is the MW of the unprocessed precursor]

CRC64: DE1D24A620496505 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MNPSDTHDHG AEASPLGQSV VYRDSYAPEL LFPIARQIKR DELGLAPDTL PFVGEDLWNA 

        70         80         90        100        110        120 
YELSWLNSRG KPVAAIGTFR VPVTSPRLIE SKSLKLYLNS FNQHRFVDPT LVHDTLTRDL 

       130        140        150        160        170        180 
STAAGAPVAV SIEVLSRRPT RVFGYPHGVL LDEIDIEIDT YRTDPAFLQS DFNADEVSET 

       190        200        210        220        230        240 
LYSHLLKSNC LVTGQPDWGT IVLRYIGPPI DRAGLLRYIV SFRSHNEFHE QCVERIFCDV 

       250        260        270        280 
LRRCAPRHLA VWARYTRRGG LDINPFRSTG HFTQFDNIAE VRQ

Q5P6G9 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools