[1]	NUCLEOTIDE SEQUENCE [MRNA]. TISSUE=Liver; Charest R., Beaudry D., Girard C., Palin M.-F.; "Interactions folic acid-vitamin B12-methionine: effects on liver metabolism and production of dairy cows."; Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.

Comments

FUNCTION: Catalyzes the conversion of 5,10-methylenetetrahydrofolate to 5-methyltetrahydrofolate, a co-substrate for homocysteine remethylation to methionine (By similarity).
CATALYTIC ACTIVITY: 5-methyltetrahydrofolate + NAD(P)⁺ = 5,10-methylenetetrahydrofolate + NAD(P)H.
COFACTOR: FAD (By similarity).
ENZYME REGULATION: Allosterically regulated by S-adenosylmethionine (By similarity).
PATHWAY: One-carbon metabolism; tetrahydrofolate pathway .
SUBUNIT: Homodimer (By similarity).
SIMILARITY: Belongs to the methylenetetrahydrofolate reductase family.

Copyright

Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms. Distributed under the Creative Commons Attribution-NoDerivs License.

Cross-references

Sequence databases

EMBL

AY854631; AAW39033.1; -; mRNA.

[EMBL / GenBank / DDBJ] [CoDingSequence]

RefSeq

NP_001011685.1; -.

UniGene

Bt.26830

3D structure databases

ModBase

Q5I598.

Ontologies

GO:0004489;	Molecular function: methylenetetrahydrofolate reductase (NADPH) activity (inferred from electronic annotation from InterPro).
GO:0055114;	Biological process: oxidation reduction (inferred from electronic annotation from UniProtKB-KW).
QuickGo view.

Family and domain databases

InterPro

IPR004621; Fadh2_euk.
IPR003171; Mehydrof_redctse.
Graphical view of domain structure.

Pfam

PF02219; MTHFR; 1.
Pfam graphical view of domain structure.

TIGRFAMs

TIGR00677; fadh2_euk; 1.

ProtoNet

Q5I598.

Genome annotation databases

Ensembl

ENSBTAG00000020698; Bos taurus. [Contig view]

GeneID

497032; -.

KEGG

bta:497032; -.

Phylogenomic databases

HOVERGEN

Q5I598; -.

Other

UniRef

View cluster of proteins with at least 50% / 90% / 100% identity.

Keywords

Allosteric enzyme; FAD; Flavoprotein; NADP; Oxidoreductase.

Features

Feature table viewer

`Key`	`From To`	`Length`		`Description`	`FTId`
`CHAIN`	`1 655`	`655`		`Methylenetetrahydrofolate reductase.`	`PRO_0000236275`

Sequence information

Length: 655 AA [This is the length of the unprocessed precursor]

Molecular weight: 74485 Da [This is the MW of the unprocessed precursor]

CRC64: 8F88D084036BD3A5 [This is a checksum on the sequence]

        10         20         30         40         50         60 
MVNEPRGNGS PGPRWEGSSS GSESSRTSSR CSTPGLDPER CERLREKMKR KMDSGDKWFS 

        70         80         90        100        110        120 
LEFFPPRTAQ GAVNLISRFD RMGAGGPLFV DVTWHPAGDP GSDKETSSMV IASTAVNYCG 

       130        140        150        160        170        180 
LETILHMTCC HQSREEITGH LNKAKQLGLK NILALRGDPI GDQWEEEEGG FNYATDLVKH 

       190        200        210        220        230        240 
IRNEFGDYFD VCVAGYPKGH PEGESFEADL KHLKEKVAAG ADFIITQLFF EAETFFRFVK 

       250        260        270        280        290        300 
ACSEIGITCP VLPGIFPIQG YHSLRQLVKL SKLEVPQQIK DVIEPIKDND AAIRNYGIEQ 

       310        320        330        340        350        360 
AVSLCQELLA SGLVPGLHFY TLNREVATIE VLKRLGLWIE DPRRPLPWAL SAHPKRRVED 

       370        380        390        400        410        420 
VRPIFWASRP KSYIYRTQEW DEFPNGRWGN SSSPAFGELK DYYLFYLKSK SPKEELLKMW 

       430        440        450        460        470        480 
GEELTSEESV FQVFAHHLSG EPNQNGYKVT CLPWNDEPLA AETSLMKEEL LRVNRRGILT 

       490        500        510        520        530        540 
INSQPNINGK PSSDPIVGWG PSGGYVFQKA YLEFFTSRET VEALLQVLKK YELRVNYHIV 

       550        560        570        580        590        600 
DVKGENITNA PELQPNAVTW GIFPGREIIQ PTVVDPVSFM FWKDEAFALW IEQWGKLYEE 

       610        620        630        640        650 
ESPSRMIIQY IHDNYFLVNL VDNEFPLDNC LWQVVEDTFE LLSRPPQDKR ETEAL

Q5I598 in FASTA format

View entry in original UniProtKB/Swiss-Prot format
View entry in raw text format (no links)
Report form for errors/updates in this UniProtKB/Swiss-Prot entry

	BLAST submission on ExPASy/SIB or at NCBI (USA)		Sequence analysis tools: ProtParam, ProtScale, Compute pI/Mw, PeptideMass, PeptideCutter, Dotlet (Java)
	ScanProsite, MotifScan		Submit a homology modeling request to SWISS-MODEL
	NPSA Sequence analysis tools