ID   LDH1_LACAC              Reviewed;         323 AA.
AC   Q5FMB0;
DT   30-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   04-NOV-2008, entry version 36.
DE   RecName: Full=L-lactate dehydrogenase 1;
DE            Short=L-LDH 1;
DE            EC=1.1.1.27;
GN   Name=ldh1; OrderedLocusNames=LBA0271;
OS   Lactobacillus acidophilus.
OC   Bacteria; Firmicutes; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=1579;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R.,
RA   Buck B.L., McAuliffe O., Souther N., Dobson A., Duong T., Callanan M.,
RA   Lick S., Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- CATALYTIC ACTIVITY: (S)-lactate + NAD(+) = pyruvate + NADH.
CC   -!- PATHWAY: Fermentation; pyruvate fermentation to lactate; (S)-
CC       lactate from pyruvate: step 1/1.
CC   -!- SUBUNIT: Homotetramer (By similarity).
CC   -!- SUBCELLULAR LOCATION: Cytoplasm (By similarity).
CC   -!- SIMILARITY: Belongs to the LDH/MDH superfamily. LDH family.
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DR   EMBL; CP000033; AAV42164.1; -; Genomic_DNA.
DR   RefSeq; YP_193195.1; -.
DR   GeneID; 3252415; -.
DR   GenomeReviews; CP000033_GR; LBA0271.
DR   KEGG; lac:LBA0271; -.
DR   NMPDR; fig|272621.3.peg.310; -.
DR   HOGENOM; Q5FMB0; -.
DR   BioCyc; LACI272621:LBA0271-MON; -.
DR   GO; GO:0005737; C:cytoplasm; IEA:HAMAP.
DR   GO; GO:0004459; F:L-lactate dehydrogenase activity; IEA:HAMAP.
DR   GO; GO:0019642; P:anaerobic glycolysis; IEA:HAMAP.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   HAMAP; MF_00488; -; 1.
DR   InterPro; IPR001557; L-lactate/malate_DHase.
DR   InterPro; IPR011304; L-lactate_DHase.
DR   InterPro; IPR001236; Lactate/malate_DHase.
DR   InterPro; IPR015955; Lactate_DHase/Glyco_Ohase_4_C.
DR   InterPro; IPR016040; NAD(P)-bd.
DR   Gene3D; G3DSA:3.90.110.10; lact_mal_DH; 1.
DR   Gene3D; G3DSA:3.40.50.720; NAD(P)-bd; 1.
DR   Pfam; PF02866; Ldh_1_C; 1.
DR   Pfam; PF00056; Ldh_1_N; 1.
DR   PIRSF; PIRSF000102; Lac_mal_DH; 1.
DR   PRINTS; PR00086; LLDHDRGNASE.
DR   TIGRFAMs; TIGR01771; L-LDH-NAD; 1.
DR   PROSITE; PS00064; L_LDH; 1.
PE   3: Inferred from homology;
KW   Complete proteome; Cytoplasm; Glycolysis; NAD; Oxidoreductase;
KW   Phosphoprotein.
FT   CHAIN         1    323       L-lactate dehydrogenase 1.
FT                                /FTId=PRO_0000237547.
FT   NP_BIND      16     44       NAD (By similarity).
FT   ACT_SITE    179    179       Proton acceptor.
FT   BINDING      92     92       Substrate (By similarity).
FT   BINDING     124    124       NAD or substrate (By similarity).
FT   BINDING     155    155       Substrate (By similarity).
FT   BINDING     232    232       Substrate (By similarity).
FT   MOD_RES     223    223       Phosphotyrosine (By similarity).
SQ   SEQUENCE   323 AA;  35039 MW;  DD2B0FFBC2ADA228 CRC64;
     MARVEKPRKV ILVGDGAVGS TFAFSMVQQG IAEELGIIDI AKEHVEGDAI DLADATPWTF
     PKNIYAADYA DCKDADLVVI TAGAPQKPGE TRLDLVNKNL KILSSIVEPV VESGFEGIFL
     VVANPVDILT HATWKISGFP KDRVIGSGTS LDTGRLQKVI GKMEHVDPRS VNAYMLGEHG
     DTEFPVWSYN NVGGVKVSDW VKAHGMDESK LEEIHKEVAD MAYDIINKKG ATFYGIGTAS
     AMIAKAILND EHRVLPLSVA MDGQYGLHDL HIGTPAVVGR NGLEQIIEMP LTADEQAKME
     ASAKQLKEVM DKAFEETGVK VRQ
//